Abstract:
:Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the control of the strong and constitutive native gpd promoter from P. eryngii. The recombinant plasmid was used to transform of P. eryngii protoplasts. Successful transformation was demonstrated by molecular analyses. Moreover, the mycelia of the transformants showed green epipolic dispersion on fluorescence microscopy. About 90-210 transformants were produced per μg plasmid DNA per 10(7) viable protoplasts.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Yin Y,Liu Y,Jin H,Wang S,Zhao S,Geng X,Li M,Xu Fdoi
10.1007/s10529-012-0985-5subject
Has Abstractpub_date
2012-10-01 00:00:00pages
1895-900issue
10eissn
0141-5492issn
1573-6776journal_volume
34pub_type
杂志文章abstract::An extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25°C. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. EstK was purified to homogeneit...
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更新日期:2012-11-01 00:00:00
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更新日期:2005-11-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2013-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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更新日期:2012-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2016-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8655-5
更新日期:2005-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1546-x
更新日期:2014-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3682-9
更新日期:2005-09-01 00:00:00