Abstract:
:ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial for LW01 biosynthesis. ECO-TE I, the gene encoding type I thioesterase from LW01 polyketide synthases, cannot complement ECO-orf27 deficient mutant distinguishing ECO-orf27 from type I thioesterase gene. Type II thioesterase gene pikAV from Streptomyces venezuelae could complement ECO-orf27 in A. orientalis indicating that the two genes are equivalent in their function. Overexpression of ECO-orf27 resulted in a 20 % increase in LW01 production providing an alternative approach for yield improvement.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Shen Y,Huang H,Zhu L,Luo M,Chen Ddoi
10.1007/s10529-012-1010-8subject
Has Abstractpub_date
2012-11-01 00:00:00pages
2087-91issue
11eissn
0141-5492issn
1573-6776journal_volume
34pub_type
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