Abstract:
:The impact of the two adaptation-induced mutations in an improved xylose-fermenting Zymomonas mobilis strain was investigated. The chromosomal mutation at the xylose reductase gene was critical to xylose metabolism by reducing xylitol formation. Together with the plasmid-borne mutation impacting xylose isomerase activity, these two mutations accounted for 80 % of the improvement achieved by adaptation. To generate a strain fermenting xylose in the presence of high acetic acid concentrations, we transferred the two mutations to an acetic acid-tolerant strain. The resulting strain fermented glucose + xylose (each at 5 % w/v) with 1 % (w/v) acetic acid at pH 5.8 to completion with an ethanol yield of 93.4 %, outperforming other reported strains. This work demonstrated the power of applying molecular understanding in strain improvement.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Agrawal M,Wang Y,Chen RRdoi
10.1007/s10529-012-0970-zsubject
Has Abstractpub_date
2012-10-01 00:00:00pages
1825-32issue
10eissn
0141-5492issn
1573-6776journal_volume
34pub_type
杂志文章abstract::The Bacillus pumilus SG2 chitinase gene (ChiS) and its truncated form lacking chitin binding (ChBD) and fibronectin type III (FnIII) domains were transformed to Arabidopsis plants and the expression, functionality and antifungal activity of the recombinant proteins were investigated. Results showed that while the two ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0192-1
更新日期:2010-04-01 00:00:00
abstract::The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0720-7
更新日期:2011-12-01 00:00:00
abstract::Fed-batch cultivation of Azotobacter vinelandii 21was optimized for biosurfactant production. Optimization of feed-rate time profile and concentration of nutrient medium components in feeding solution is based on a hybrid mathematical model consisting of mass-balance equations for biomass, biosurfactant, volume of cul...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000035486.81463.b4
更新日期:2004-07-01 00:00:00
abstract::Lymphotactin-XCL1 is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells. We expressed human lymphotactin (LTN) by the lactic-acid bacterium Lactococcus lactis. Biological activity of LTN was confirmed by chemo-attraction of human T-cel...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9855-6
更新日期:2009-02-01 00:00:00
abstract:OBJECTIVES:To examine the synergistic antibacterial activity of violacein and silver nanoparticles (AgNPs) against ATCC bacteria, Staphylococcus aureus, Escherichia coli and two bacteria isolated from bovine mastitis. METHODS:Violacein from Chromobacterium violaceum and biogenic AgNPs from Fusarium oxysporum were eval...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02745-8
更新日期:2019-12-01 00:00:00
abstract::Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0428-0
更新日期:2011-02-01 00:00:00
abstract::Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytube...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023038804556
更新日期:2003-04-01 00:00:00
abstract::Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6)...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1546-x
更新日期:2014-09-01 00:00:00
abstract::At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1843-z
更新日期:2015-08-01 00:00:00
abstract::Human DNA polymerase iota (Pol ι) is a Y-family DNA polymerase with unusual biochemical properties and not fully understood functions. Pol ι preferentially incorporates dGTP opposite template thymine. This property can be used to monitor Pol ι activity in the presence of other DNA polymerases, e.g. in cell extracts of...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0764-8
更新日期:2012-02-01 00:00:00
abstract::A novel epoxide hydrolase (EHase) from polycyclic aromatic hydrocarbon (PAH)-degrading bacteria was identified and characterized. EHase activity was identified in four strains of PAH-degrading bacteria isolated from commercial gasoline and oil-contaminated sediment based on their growth on styrene oxide and its deriva...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1114-1
更新日期:2013-04-01 00:00:00
abstract::Alginate oligosaccharide (AOS) was reported to possess antioxidant and free radical scavenging activities, but the specific effects and mechanisms remain unclear. We investigated the effects of AOS on H2O2-induced oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) and the associated mech...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03010-z
更新日期:2020-12-01 00:00:00
abstract::Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9973-9
更新日期:2009-07-01 00:00:00
abstract::By combining gene design and heterologous over-expression of Rhodotorula gracilis D-amino acid oxidase (RgDAO) in Pichia pastoris, enzyme production was enhanced by one order of magnitude compared to literature benchmarks, giving 350 kUnits/l of fed-batch bioreactor culture with a productivity of 3.1 kUnits/l h. P. pa...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0456-9
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVES:To improve the thermostability and catalytic property of a mesophilic 1,3-1,4-β-glucanase by combinational mutagenesis and to test its effect in congress mashing. RESULTS:A mutant β-glucanase (rE-BglTO) constructed by combinational mutagenesis showed a 25 °C increase in optimal temperature (to 70 °C) a 19.5...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2212-2
更新日期:2017-01-01 00:00:00
abstract::Of several types of chemical surfactants and biosurfactants, only the culture broth of Bacillus subtilis C1 containing surfactin at 10 mg l(-1) completely inhibited the growth of Microcystis aeruginosa, a bloom-forming cyanobacterium in highly eutrophic lakes. The broth with 10 mg surfactin l(-1) also removed 85% of t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024508927361
更新日期:2003-07-01 00:00:00
abstract::A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The dete...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9472-9
更新日期:2007-12-01 00:00:00
abstract:OBJECTIVE:To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. RESULTS:Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate,...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2101-8
更新日期:2016-08-01 00:00:00
abstract::High concentrations of 2,4,6-trinitrotoluene (TNT) and related nitroaromatic compounds are commonly found in soil and groundwater at former explosive plants. The bacterium, Raoultella terrigena strain HB, isolated from a contaminated site, converts TNT into the corresponding amino products. Radio-HPLC analysis with [(...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9244-y
更新日期:2007-03-01 00:00:00
abstract::Rhizopus arrhizus, strain DAR 36017, produced L(+)-lactic acid in a simultaneous saccharification and fermentation process using starch waste effluents. Lactic acid at 19.5-44.3 g l(-1) with a yield of 0.85-0.96 g g(-1) was produced in 40 h using 20-60 g starch l(-1). Supplementation of nitrogen source may be unnecess...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000004389.53388.d0
更新日期:2003-12-01 00:00:00
abstract::Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparation method. Solid phase extraction (SPE) was employed to isolate the plant metabolites for nuclear magnetic resonance (NMR) analysis and to subsequently detect the differences between yeast extract elicited and control cell...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9785-3
更新日期:2008-11-01 00:00:00
abstract::Human phospholipid scramblase 1 (hPLSCR1) scrambles plasma membrane phospholipids during cell activation, blood coagulation and apoptosis. It was over-expressed in E. coli with a histidine tag and purified from the inclusion bodies (*30 mg/l culture broth) under denaturing conditions using 8 M urea. The denatured hPLS...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9797-z
更新日期:2008-12-01 00:00:00
abstract::Genetically-coded, fluorescence resonance energy transfer (FRET) biosensors are widely used to study molecular events from single cells to whole organisms. They are unique among biosensors because of their spontaneous fluorescence and targeting specificity to both organelles and tissues. In this review, we discuss the...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-006-9193-5
更新日期:2006-12-01 00:00:00
abstract::A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more than four times th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0184-1
更新日期:2010-04-01 00:00:00
abstract::Cellobiose is a major intermediate from cellulase hydrolysis of pretreated plant biomass. Engineering biocatalysts for direct use of cellobiose could eliminate the need for exogenous β-glucosidase. Additionally, rapid removal of cellobiose in a simultaneous saccharification and fermentation facilitates enzymatic hydro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1355-7
更新日期:2014-02-01 00:00:00
abstract::The current study aimed to assess the inhibitory effect of a DNA aptamer (Apt17) which targeted Salmonella invasion proteinA (SipA). The effect of Apt17, on biofilm formation by two Salmonella enteritidis strains, was tested either separately or in combination with ampicillin at different Sub MIC concentrations. Maxim...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02920-2
更新日期:2020-10-01 00:00:00
abstract:OBJECTIVE:To propose and verify a hypothesis that miR-17-5p knockdown may mitigate atherosclerotic lesions using atherosclerotic ApoE-/- mice as serum microRNA-17-5p (miR-17-5p) is elevated in patients with atherosclerosis. RESULTS:The level of miR-17-5p was higher while the level of very low density lipoprotein recep...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2337-y
更新日期:2017-07-01 00:00:00
abstract::Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000015424.04776.55
更新日期:2004-02-01 00:00:00
abstract::The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1709-9
更新日期:2015-03-01 00:00:00
abstract::Deacetoxy/deacetylcephalosporin C synthase (acDAOC/DACS) from Acremonium chrysogenum is a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C. The R308 residue located in close proximity to the C-terminus ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0504-5
更新日期:2011-04-01 00:00:00