Abstract:
:Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytuberol) detected in elicitor-treated hairy root cultures, rishitin (213 micrograms g-1 dry wt) was the most predominant followed by lubimin (171 micrograms g-1 dry wt). The elicitors also induced LOX activity (25-fold increase) and LOX metabolites, mainly 9-hydroxyoctadecadienoic acid and 9-hydroxyoctadecatrienoic acid, in potato hairy root cultures. The combination of fungal elicitor plus cyclodextrin was the most effective elicitor treatment, followed by methyl jasmonate plus cyclodextrin in inducing sesquiterpenes and LOX metabolites.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Komaraiah P,Reddy GV,Reddy PS,Raghavendra AS,Ramakrishna SV,Reddanna Pdoi
10.1023/a:1023038804556keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
593-7issue
8eissn
0141-5492issn
1573-6776journal_volume
25pub_type
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