Production of trans-10,cis-12-conjugated linoleic acid using permeabilized whole-cell biocatalyst of Yarrowia lipolytica.

abstract：:Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in confo...

journal_title：Biotechnology letters

authors： Liang P,Qin B,Mu M,Zhang X,Jia X,You S

更新日期：2013-09-01 00:00:00

abstract：:1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l(-1) and 16 g 2,3-butanediol l(-1) w...

journal_title：Biotechnology letters

authors： Cheng KK,Liu DH,Sun Y,Liu WB

更新日期：2004-06-01 00:00:00

abstract：:UV-induced DNA damage plays a key role in the etiology of certain diseases. The ability of blueberry anthocyanins and anthocyanidins (BA) to protect cellular DNA from UV-induced damage was investigated. BA were extracted by water (BAW), ethanol (BAE) or methanol (BAM). These extracts partially restored proliferation o...

journal_title：Biotechnology letters

authors： Liu W,Lu X,He G,Gao X,Li M,Wu J,Li Z,Wu J,Wang J,Luo C

更新日期：2013-04-01 00:00:00

abstract：:MicroRNA (miRNA) profiling is a first important step in elucidating miRNA functions. Real time quantitative PCR (RT-qPCR) and microarray hybridization approaches as well as ultra high throughput sequencing of miRNAs (small RNA-seq) are popular and widely used profiling methods. All of these profiling approaches face s...

journal_title：Biotechnology letters

authors： Meyer SU,Pfaffl MW,Ulbrich SE

更新日期：2010-12-01 00:00:00

abstract：:The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene su...

journal_title：Biotechnology letters

authors： Lim HA,Raku T,Tokiwa Y

更新日期：2005-04-01 00:00:00

abstract：:Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...

journal_title：Biotechnology letters

authors： Watanabe K,Noda K,Maruhashi K

更新日期：2003-05-01 00:00:00

abstract：:5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...

journal_title：Biotechnology letters

authors： Sonhom R,Thepsithar C,Jongsareejit B

更新日期：2012-09-01 00:00:00

abstract：:A new ribonuclease (RNase) with tobacco mosaic virus inhibition was isolated and purified from Bacillus cereus ZH14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of DEAE-Sephadex A-50 column, and gel chromatography of Sephacryl S-200HR column. The enzyme was purified approximatel...

journal_title：Biotechnology letters

authors： Zhou WW,Niu TG

更新日期：2009-01-01 00:00:00

abstract：:A computational search was carried out to identify additional binding sites for the manganese response regulator, ManR, in the genome of Anabaena sp. PCC 7120. This approach predicted ManR binding sites: the promoter regions of the genes of all3575-alr3576 and the gene of alr5134 from Anabaena sp. PCC 7120. Electropho...

journal_title：Biotechnology letters

authors： Huang W,Wu QY

更新日期：2004-09-01 00:00:00

abstract：:Error-prone PCR was used to create more thermoactive and/or thermostable variants of thermoalkalophilic lipases. A variant of the α6 helix (lid domain), with an 189E to V substitution at residue 189, lost its thermostability but exhibited higher activity than that of its wild-type predecessor (r03Lip). Site-saturation...

journal_title：Biotechnology letters

authors： Shih TW,Pan TM

更新日期：2011-09-01 00:00:00

abstract：:Ramoplanin is a lipopeptide antibiotic active against multi-drug-resistant, Gram-positive pathogens. Structurally, it contains a di-mannose moiety attached to the peptide core at Hpg(11). The biosynthetic gene cluster of ramoplanin has already been reported and the assembly of the depsipeptide has been elucidated but ...

journal_title：Biotechnology letters

authors： Chen JS,Wang YX,Shao L,Pan HX,Li JA,Lin HM,Dong XJ,Chen DJ

更新日期：2013-09-01 00:00:00

abstract：:Bioreactors are essential in tissue engineering, not only because they provide an in vitro environment mimicking in vivo conditions for the growth of tissue substitutes, but also because they enable systematic studies of the responses of living tissues to various mechanical and biochemical cues. The basic principles o...

journal_title：Biotechnology letters

authors： Chen HC,Hu YC

更新日期：2006-09-01 00:00:00

abstract：:Most animal cell culture media can be buffered using bicarbonate and high pressure CO(2) in a closed system. However, in an open system, the pH of the culture media increases continuously due to the marked difference in CO(2) pressure between the culture media and the atmosphere. Therefore, it is important to measure ...

journal_title：Biotechnology letters

authors： Jang J,Moon SJ,Hong SH,Kim IH

更新日期：2010-11-01 00:00:00

abstract：:A new cDNA (Os beta fruct2) encoding an acid beta-D-fructofuranosidase from rice has been cloned, sequenced and expressed in Pichia pastoris. The full-length cDNA is 2453 base pairs long and encodes a pre-pro-protein of 682 amino acids. The cDNA fragment coding for mature enzyme was sub-cloned into vector pPICZalphaA ...

journal_title：Biotechnology letters

authors： Fu RH,Wang YL,Sung HY

更新日期：2003-03-01 00:00:00

abstract：:β-Xylosidase from Thermoanaerobacterium thermosaccharolyticum was purified by His-trap affinity chromatography giving a specific activity of 5.15 U mg(-1). From gel-filtration chromatography, the purified enzyme was a tetramer with a total molecular mass of 245 kDa. Maximal enzyme activity using o-nitrophenyl(NP)-β-D-...

journal_title：Biotechnology letters

authors： Shin KC,Seo MJ,Oh DK

更新日期：2014-11-01 00:00:00

abstract：OBJECTIVE:The effects of a brief (3 days) and prolonged (6 days) period of incubation in darkness and light on the biomass content, lipid content and fatty acid profile in Chlorella vulgaris UMT-M1 were determined. RESULTS:Three days of incubation in darkness increased saturated fatty acid (SFA) content from 34.0 to 4...

journal_title：Biotechnology letters

authors： Cha TS,Yee W,Phua PSP,Loh SH,Aziz A

更新日期：2021-01-12 00:00:00

abstract：:The production of a low-temperature alkalophilic lipase from Acinetobacter johnsonii was improved using genome shuffling. The starting populations, obtained by UV irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. The optimal conditions for protoplast formation and regeneration...

journal_title：Biotechnology letters

authors： Wang H,Zhang J,Wang X,Qi W,Dai Y

更新日期：2012-01-01 00:00:00

abstract：PURPOSE OF WORK:The biosynthetic pathway of a new antitumor compound, haloroquinone, is elucidated to facilitate metabolic regulation for product accumulation and modification to produce new bioactive structural analogues of the compound. The biosynthetic origin of a novel promising protein kinase B inh...

journal_title：Biotechnology letters

authors： Niu C,Cai M,Zhang Y,Zhou X

更新日期：2012-11-01 00:00:00

abstract：OBJECTIVE:To produce (S)-3-hydroxy-1-(3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-4-(2,4,5-trifluorophenyl)butan-1-one (S)-1 from 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro [1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl)-1-(2,4,5-trifluorophenyl)butan-2-one (2) by microbial bioreduction. RESULTS:A new...

journal_title：Biotechnology letters

authors： Wei Y,Xia S,He C,Xiong W,Xu H

更新日期：2016-05-01 00:00:00

abstract：:A fully contained and efficient heterologous protein production system was designed using Brassica rapa rapa (turnip) hairy roots. Two expression cassettes containing a cauliflower mosaic virus (CaMV) 35S promoter with a duplicated enhancer region, an Arabidopsis thaliana sequence encoding a signal peptide and the CaM...

journal_title：Biotechnology letters

authors： Huet Y,Ekouna JP,Caron A,Mezreb K,Boitel-Conti M,Guerineau F

更新日期：2014-01-01 00:00:00

abstract：OBJECTIVES:To optimize the production of active inclusion bodies (IBs) containing human D-amino acid oxidase (hDAAO) in Escherichia coli. RESULTS:The optimized initial codon region combined with the coexpressed rare tRNAs, fusion of each of the N-terminal partners including cellulose-binding module, thioredoxin, gluta...

journal_title：Biotechnology letters

authors： Wang W,Sun J,Xiao W,Jiang L,Wang R,Fan J

更新日期：2017-11-01 00:00:00

abstract：:Clostridium spp. produce n-butanol in the acetone/butanol/ethanol process. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, the low product yield, toxicity to production strain, multiple-end products and downstream processing of alcohol mixt...

journal_title：Biotechnology letters

authors： Jurgens G,Survase S,Berezina O,Sklavounos E,Linnekoski J,Kurkijärvi A,Väkevä M,van Heiningen A,Granström T

更新日期：2012-08-01 00:00:00

abstract：:The shuttle vector pGYC4α (6,157 bp) was constructed based on the sigma-replicon plasmid pYC2 from Lactobacillus sakei BM5 isolated from kimchi. The vector contained inserts of the ColE1 replicon, α-amylase gene from Bacillus licheniformis containing its own signal peptide, and lactococcal promoter P32. Transformation...

journal_title：Biotechnology letters

authors： Yang EJ,Chang HC

更新日期：2011-03-01 00:00:00

abstract：:Biosurfactants could potentially outperform traditional surfactants in many applications whilst being more sustainable to source, manufacture, use and dispose of. However, currently available fermentation production methods are too inefficient to manufacture biosurfactants for these high volume markets. Foaming in an ...

journal_title：Biotechnology letters

authors： Winterburn JB,Martin PJ

更新日期：2012-02-01 00:00:00

abstract：:The culture broth of Streptomyces halstedii AJ-7 suppressed the growth of Phytophthora capsici, which causes phytophthora blight in red-peppers, with less than 1% survival of the pathogen after 12 h of treatment. The low molecular fraction (< or = 10 kDa) of the culture broth retained anti-fungal activity against P. c...

journal_title：Biotechnology letters

authors： Joo GJ

更新日期：2005-02-01 00:00:00

abstract：:Human phospholipid scramblase 1 (hPLSCR1) scrambles plasma membrane phospholipids during cell activation, blood coagulation and apoptosis. It was over-expressed in E. coli with a histidine tag and purified from the inclusion bodies (*30 mg/l culture broth) under denaturing conditions using 8 M urea. The denatured hPLS...

journal_title：Biotechnology letters

authors： Sahu SK,Gopala Krishna A,Gummadi SN

更新日期：2008-12-01 00:00:00

abstract：:Efforts to elucidate the relationships between microorganisms and metal corrosion were mainly directed to understanding the formation of biofilm structures grown on corroded surfaces. The emergence of high throughput DNA sequencing techniques has helped in the description of microbial species involved directly and ind...

journal_title：Biotechnology letters

authors： Procópio L

更新日期：2020-03-01 00:00:00

abstract：:Feruloylated mono- and di-acylglycerols were synthesized in a two step reaction: ethyl ferulate was first transesterified with glycerol and then this was esterified with oleic acid. The yield of the combined feruloylated mono- and di-acylglycerols in the second reaction reached 96% when esterification of 0.3 g transes...

journal_title：Biotechnology letters

authors： Sun S,Shan L,Liu Y,Jin Q,Wang X,Wang Z

更新日期：2007-12-01 00:00:00

abstract：:A novel two-step fermentation process was developed to enhance arachidonic acid (ARA) production by Mortierella alpina ME-1 in a 5 l fermentor. Agitation speed and aeration rate were adjusted from 180 to 40 rpm and from 0.6 to 1 vvm, respectively, after 5 days cultivation, to decrease physical damage to the mycelia an...

journal_title：Biotechnology letters

authors： Jin MJ,Huang H,Xiao AH,Zhang K,Liu X,Li S,Peng C

更新日期：2008-06-01 00:00:00

abstract：:Lentiviral vectors have been used for gene transfer into the liver but their ability to efficiently transduce quiescent hepatocytes remains controversial. Lentivirus-mediated gene transfer is more efficient in cycling cells. We determine the effect of H-IL6 in the lentiviral transduction. The lentiviral vector was use...

journal_title：Biotechnology letters

authors： Picanço-Castro V,Fontes AM,Heinz S,Tonn T,Covas DT

更新日期：2008-02-01 00:00:00