Abstract:
PURPOSE OF WORK:The biosynthetic pathway of a new antitumor compound, haloroquinone, is elucidated to facilitate metabolic regulation for product accumulation and modification to produce new bioactive structural analogues of the compound. The biosynthetic origin of a novel promising protein kinase B inhibitor and anti-tumor compound, haloroquinone, from a marine-derived fungus, Halorosellinia sp. was clarified. The origin of carbon skeleton of haloroquinone was elucidated by feeding experiments with [2-(13)C]malonate and [1,2,3-(13)C(3)]malonate followed by (13)C-NMR analysis of the isolated compounds: 15 carbon atoms were derived from malonate, of which eight were from the methylene group and seven from the carboxyl group. The remaining one is probably obtained by O-methylation. Haloroquinone is thus synthesized via a polyketide pathway using malonyl-CoA as both the starter unit and the extender unit.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Niu C,Cai M,Zhang Y,Zhou Xdoi
10.1007/s10529-012-1019-zsubject
Has Abstractpub_date
2012-11-01 00:00:00pages
2119-24issue
11eissn
0141-5492issn
1573-6776journal_volume
34pub_type
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