The chimeric cytokine Hyper-IL-6 enhances the efficiency of lentiviral gene transfer in hepatocytes both in vitro and in vivo.

abstract：:Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...

journal_title：Biotechnology letters

authors： Minkner R,Park EY

更新日期：2018-04-01 00:00:00

abstract：:Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is used clinically to treat leucopenia typically caused by cancer chemotherapy or radiotherapy. This study used multiple strategies to obtain very high expression levels of OsrhGM-CSF (14 microg/seed) in rice endosperm. Electron micrographs of immunogold...

journal_title：Biotechnology letters

authors： Ning T,Xie T,Qiu Q,Yang W,Zhou S,Zhou L,Zheng C,Zhu Y,Yang D

更新日期：2008-09-01 00:00:00

abstract：OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...

journal_title：Biotechnology letters

authors： Do DTH,Fickers P,Ben Tahar I

更新日期：2021-02-01 00:00:00

abstract：:16,17-Epoxysterol plays an important role in pharmaceutical steroid synthesis. To investigate the potential application of cytochrome P450 for epoxysterol synthesis, an approach to the epoxidation of 16,17-epoxysterol, based on directed evolution of cytochrome P450 BM-3, was developed. This comprised random gene mutag...

journal_title：Biotechnology letters

authors： Jiang D,Tu R,Bai P,Wang Q

更新日期：2013-10-01 00:00:00

abstract：:A mutant ofS. chrysomallus with a D-glucose isomerase inducible by D-glucose or its catabolites was characterized. In contrast to the wild-type strain it showed a decreased catabolite repression by D-glucose of D-xylose consumption and a constitutive pentose phosphate pathway as well. A hypothesis concerning altered i...

journal_title：Biotechnology letters

authors： Hänel F,Roth M,Neigenfind M,Gräfe U,Bormann EJ

更新日期：1987-12-01 00:00:00

abstract：:Non-viral vector, pCMV-Pth, with full-length human parathyroid hormone (prepro-hPTH) cDNA was constructed and delivered into the ovariectomized mouse quadriceps to explore the effects of electroporational gene transfer of PTH on the bone. The expression of hPTH in the transfected mice was detected by RT-PCR and radioi...

journal_title：Biotechnology letters

authors： Liu B,Tang J,Ji J,Gu J

更新日期：2003-12-01 00:00:00

abstract：:A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly conserved among serine...

journal_title：Biotechnology letters

authors： Xie Z,Xu B,Ding J,Liu L,Zhang X,Li J,Huang Z

更新日期：2013-08-01 00:00:00

abstract：:Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the cont...

journal_title：Biotechnology letters

authors： Yin Y,Liu Y,Jin H,Wang S,Zhao S,Geng X,Li M,Xu F

更新日期：2012-10-01 00:00:00

abstract：OBJECTIVES:To display a recombinant avidin fused to the autotransporter ShdA to bind biotinylated molecules on the surface of Escherichia coli. RESULTS:Two chimeric protein constructs containing avidin fused to the autotransporter ShdA were expressed on the surface of Escherichia coli DH5α. One fusion protein containe...

journal_title：Biotechnology letters

authors： Pardavé-Alejandre HD,Alvarado-Yaah JE,Pompa-Mera EN,Muñoz-Medina JE,Sárquiz-Martínez B,Santacruz-Tinoco CE,Manning-Cela RG,Ortíz-Navarrete V,López-Macías C,González-Bonilla CR

更新日期：2018-03-01 00:00:00

abstract：:Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...

journal_title：Biotechnology letters

authors： Zheng ZM,Xu YZ,Wang TP,Dong CQ,Yang YP,Liu DH

更新日期：2010-02-01 00:00:00

abstract：:The culture broth of Streptomyces halstedii AJ-7 suppressed the growth of Phytophthora capsici, which causes phytophthora blight in red-peppers, with less than 1% survival of the pathogen after 12 h of treatment. The low molecular fraction (< or = 10 kDa) of the culture broth retained anti-fungal activity against P. c...

journal_title：Biotechnology letters

authors： Joo GJ

更新日期：2005-02-01 00:00:00

abstract：:Growth of Streptococcus zooepidemicus in a 10 l bioreactor with 50 g sucrose/l and 10 g casein hydrolysate/l gave 5-6 g hyaluronic acid/l after 24-28 h. Purification of hyaluronic acid gave a recovery of 65% with the final material having an Mr of approximately 4 x 10(6) Da with less than 0.1% protein. ...

journal_title：Biotechnology letters

authors： Rangaswamy V,Jain D

更新日期：2008-03-01 00:00:00

abstract：OBJECTIVES:To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. RESULTS:HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung canc...

journal_title：Biotechnology letters

authors： Zhang Y,Yang L,Ling C,Heng W

更新日期：2018-04-01 00:00:00

abstract：:The combined analysis of peptide mass fingerprinting and 2-DE/MS using the induced and selected protein spots following growth of Pseudomonas sp. DU102 on benzoate or p-hydroxybenzoate revealed not only alpha- and beta-subunits of protocatechuate 3,4-dioxygenase but also catechol 1,2-dioxygenase responsible for ortho-...

journal_title：Biotechnology letters

authors： Kim SI,Park SH,Kim JW,Leem SH,Shin DJ,Kim SH,Lee DH,Kahng HY

更新日期：2007-10-01 00:00:00

abstract：:Staphylokinase (SAK) is a promising thrombolytic agent for treating blood-clotting disorders. Recombinant SAK (rSAK) was produced after integration of the gene into Pichia pastoris genome. The recombinant Pichia carrying multiple insertions of the SAK gene yielded high-level (approximately 1 g/l) of extracellular glyc...

journal_title：Biotechnology letters

authors： Apte-Deshpnade A,Mandal G,Soorapaneni S,Prasad B,Kumar J,Padmanabhan S

更新日期：2009-06-01 00:00:00

abstract：:An on-line device, ORP (oxidation-reduction potential)-stat, was used to control glucose-feeding for enhancing xylitol conversion from D-xylose during an oxygen-limited fermentation by Candida tropicalis. The fermentation was carried out in a 5 l jar fermenter. After glucose in the medium was depleted, a switching to ...

journal_title：Biotechnology letters

authors： Sheu DC,Duan KJ,Jou SR,Chen YC,Chen CW

更新日期：2003-12-01 00:00:00

abstract：PURPOSE OF WORK:The purpose of this study was to determine if Arabidopsis protoplast transfection could be scaled up, from the commonly used cell-based studies, to be used in triterpenoid production assays as an in planta alternative/complement to other expression systems. Enzyme activities are often identified using h...

journal_title：Biotechnology letters

authors： Johnson EE,Jetter R,Wasteneys G

更新日期：2014-04-01 00:00:00

abstract：OBJECTIVES:Marc-145 cells (monkey embryonic kidney epithelial cells) play a critical role in the biotechnology industry as certain virus host cells. To investigate the expression of enhanced green fluorescent protein (eGFP) gene as a foreign gene in Marc-145 cells, which we developed an approach of foreign gene site-sp...

journal_title：Biotechnology letters

authors： Chang Y,Shao J,Gao Y,Liu W,Gao Z,Hu Y,Chang H

更新日期：2020-08-01 00:00:00

abstract：OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...

journal_title：Biotechnology letters

authors： Savvides AL,Moisi K,Katsifas EA,Karagouni AD,Hatzinikolaou DG

更新日期：2019-05-01 00:00:00

abstract：:The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respective...

journal_title：Biotechnology letters

authors： van der Valk AM,Howbrook DN,O'Shaughnessy MC,Sarker DK,Baker SC,Louwrier A,Lloyd AW

更新日期：2003-08-01 00:00:00

abstract：:Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidas...

journal_title：Biotechnology letters

authors： Wu JM,Hsu TA,Lee CK

更新日期：2003-09-01 00:00:00

abstract：:Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparation method. Solid phase extraction (SPE) was employed to isolate the plant metabolites for nuclear magnetic resonance (NMR) analysis and to subsequently detect the differences between yeast extract elicited and control cell...

journal_title：Biotechnology letters

authors： Fraccaroli M,Nicoletti S,Maltese F,Choi YH,Guzzo F,Levi M,Verpoorte R

更新日期：2008-11-01 00:00:00

abstract：OBJECTIVES:To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system. RESULTS:An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM c...

journal_title：Biotechnology letters

authors： Jin L,Wang ZS,Cao Y,Sun RQ,Zhou H,Cao RY

更新日期：2021-02-01 00:00:00

abstract：OBJECTIVE:Chinese hamster ovary (CHO) cells are the leading cell factories for producing recombinant proteins in the biopharmaceutical industry. In this regard, constraint-based metabolic models are useful platforms to perform computational analysis of cell metabolism. These models need to be regularly updated in order...

journal_title：Biotechnology letters

authors： Fouladiha H,Marashi SA,Li S,Li Z,Masson HO,Vaziri B,Lewis NE

更新日期：2021-01-01 00:00:00

abstract：:Chloroperoxidase from Caldariomyces fumago catalyses the oxidation of 1,2-dihydronaphthalene to (1R,2R)-(+)-dihydroxytetrahydronaphthalene in homogenous citrate buffer/ionic liquid mixtures, using t-butyl hydroperoxide as O2 donor. It tolerates up to 30 (v/v) 1,3-dimethylimidazolium methylsulfate or 1-butyl-3-methylim...

journal_title：Biotechnology letters

authors： Sanfilippo C,D'Antona N,Nicolosi G

更新日期：2004-12-01 00:00:00

abstract：:We assessed the whole genome amplification strategy, known as multiple displacement amplification (MDA), for use with the TaqMan genotyping platform for DNA samples derived from two case-control studies nested in the Nurses' Health Study and the Physicians' Health Study. Our objectives were to (1) quantify DNA yield f...

journal_title：Biotechnology letters

authors： Tranah GJ,Lescault PJ,Hunter DJ,De Vivo I

更新日期：2003-07-01 00:00:00

abstract：:Degradation of lignocellulose with pressurised hot water is an efficient method of bioethanol production. However, the resultant solution inhibits ethanol fermentation by Saccharomyces cerevisiae. Here, we first report that glycolaldehyde, which is formed when lignocellulose is treated with pressurised hot water, inhi...

journal_title：Biotechnology letters

authors： Jayakody LN,Hayashi N,Kitagaki H

更新日期：2011-02-01 00:00:00

abstract：:In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...

journal_title：Biotechnology letters

authors： Su M,Li Y,Ge X,Tian P

更新日期：2015-03-01 00:00:00

abstract：OBJECTIVES:Regenerative medicine approaches using reprogrammed or transdifferentiated cells require efficient single cell expression profiling to analyze culture homogeneity for quality control and recipients' safety. RESULTS:While antigen-antibody based systems have been developed for several proteins, probing at the...

journal_title：Biotechnology letters

authors： Kraus P,Kocsis V,Williams C,Youngs B,Lufkin T

更新日期：2015-08-01 00:00:00

abstract：:Genomic DNA was isolated from as little as 2 mg dry biomass of Magnaporthe grisea by microwave treatment within 30 s. The quantity of DNA was good enough for PCR analysis and Dot blot hybridization. This technique can be used for various studies, such as DNA fingerprinting to study the population structure of the phyt...

journal_title：Biotechnology letters

authors： Tendulkar SR,Gupta A,Chattoo BB

更新日期：2003-11-01 00:00:00