Directed evolution of cytochrome P450 for sterol epoxidation.

abstract：:Chloroperoxidase from Caldariomyces fumago catalyses the oxidation of 1,2-dihydronaphthalene to (1R,2R)-(+)-dihydroxytetrahydronaphthalene in homogenous citrate buffer/ionic liquid mixtures, using t-butyl hydroperoxide as O2 donor. It tolerates up to 30 (v/v) 1,3-dimethylimidazolium methylsulfate or 1-butyl-3-methylim...

journal_title：Biotechnology letters

authors： Sanfilippo C,D'Antona N,Nicolosi G

更新日期：2004-12-01 00:00:00

abstract：:An extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25°C. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. EstK was purified to homogeneit...

journal_title：Biotechnology letters

authors： Hong S,Lee C,Jang SH

更新日期：2012-06-01 00:00:00

abstract：:A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast...

journal_title：Biotechnology letters

authors： Takagi M,Kitabayashi T,Koizumi S,Hirose H,Kondo S,Fujiwara M,Ueno K,Hiroaki M,Hosokawa Y,Masuhara H,Wakitani S

更新日期：2008-07-01 00:00:00

abstract：OBJECTIVES:To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line. RESULTS:When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recomb...

journal_title：Biotechnology letters

authors： Shen X,Dojcinovic D,Baldi L,Hacker DL,Luescher IF,Wurm FM

更新日期：2018-01-01 00:00:00

abstract：:Oligo-chitosans were chemically modified with dinitrophenyl groups for selective and rapid adsorption of amitriptyline by forming pi-pi complexes. 1H-NMR was utilized not only for characterization of modified chitosans but also for monitoring the aromatic-aromatic interaction. The variation in the chemical shift of ar...

journal_title：Biotechnology letters

authors： Lee DW,Baney R

更新日期：2004-05-01 00:00:00

abstract：:Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...

journal_title：Biotechnology letters

authors： Cerezo J,Targovnik AM,Smith ME,González Maglio D,Luppo VC,Morales MA,Miranda MV,Rodríguez Talou J

更新日期：2020-03-01 00:00:00

abstract：:Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63 kDa α-subunit and a 95 kDa β subunit. Moloney murine leukaemia virus reverse transcriptase (MMLV RT) is a 75 kDa monomer. These two RTs are the most extensively used for conversion of RNA to DNA. We previously developed sever...

journal_title：Biotechnology letters

authors： Konishi A,Yasukawa K,Inouye K

更新日期：2012-07-01 00:00:00

abstract：OBJECTIVES:To investigate mycothiol peroxidase (MPx) of Corynebacterium glutamicum that is a novel CysGPx family peroxidase using both the mycoredoxin and thioredoxin reducing systems as proton donors for peroxide detoxification and may be involved in the relief of acid stress. RESULTS:A Δmpx mutant exhibited signific...

journal_title：Biotechnology letters

authors： Wang T,Gao F,Kang Y,Zhao C,Su T,Li M,Si M,Shen X

更新日期：2016-07-01 00:00:00

abstract：OBJECTIVE:To evaluate the role and the molecular mechanism of miR-30d in non-small cell lung cancer (NSCLC). RESULTS:qRT-PCR was used to detect miR-30d expression in NSCLC tissues and cell lines. miR-30d was frequently down-regulated in NSCLC and its expression was associated with clinicopathological features of NSCLC...

journal_title：Biotechnology letters

authors： Wu Y,Zhang J,Hou S,Cheng Z,Yuan M

更新日期：2017-12-01 00:00:00

abstract：OBJECTIVES:To acquire a thermostable xylanase, that is suitable for xylooligosaccharide production from pretreated corncobs, the metagenomic method was used to obtain the gene from an uncultured environmental microorganism. RESULTS:A thermostable xylanase-encoding gene (xyn10CD18) was cloned directly from the metageno...

journal_title：Biotechnology letters

authors： Sun MZ,Zheng HC,Meng LC,Sun JS,Song H,Bao YJ,Pei HS,Yan Z,Zhang XQ,Zhang JS,Liu YH,Lu FP

更新日期：2015-09-01 00:00:00

abstract：:Rhizopus arrhizus, strain DAR 36017, produced L(+)-lactic acid in a simultaneous saccharification and fermentation process using starch waste effluents. Lactic acid at 19.5-44.3 g l(-1) with a yield of 0.85-0.96 g g(-1) was produced in 40 h using 20-60 g starch l(-1). Supplementation of nitrogen source may be unnecess...

journal_title：Biotechnology letters

authors： Jin B,Huang LP,Lant P

更新日期：2003-12-01 00:00:00

abstract：OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...

journal_title：Biotechnology letters

authors： Do DTH,Fickers P,Ben Tahar I

更新日期：2021-02-01 00:00:00

abstract：:Sodium butyrate is commonly used in mammalian cell cultures to increase the productivity of recombinant proteins. A Chinese hamster ovary (CHO) cell line producing recombinant human erythropoietin (rhEPO) was cultured in commercial medium. Addition of 0.5 mM butyrate inhibited the over-growth of the cells after the me...

journal_title：Biotechnology letters

authors： Liu Y,Zhou X,Song Z,Zhang Y

更新日期：2014-05-01 00:00:00

abstract：:Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in confo...

journal_title：Biotechnology letters

authors： Liang P,Qin B,Mu M,Zhang X,Jia X,You S

更新日期：2013-09-01 00:00:00

abstract：:Electroporation of Litopenaeus schmitti embryos was used to transfer the pE300tiGH15 plasmid that contains the tilapia growth hormone gene (tiGH) complexed with a nuclear localization signal peptide into the zygotes. The gene construct was detected in 35 (36%) of the 98 larvae screened by PCR and Southern blot analyse...

journal_title：Biotechnology letters

authors： Arenal A,Pimentel R,Pimentel E,Martín L,Santiesteban D,Franco R,Aleström P

更新日期：2008-05-01 00:00:00

abstract：OBJECTIVES:To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression. RESULTS:The efficiency of three different 5'-regulatory sequences to achieve transient expression of t...

journal_title：Biotechnology letters

authors： Gerasymenko IM,Sheludko YV

更新日期：2017-07-01 00:00:00

abstract：:Cell-free extracts of Streptomyces sp. DRS I converted ampicillin to cephalexin, presumably due to the activity of the enzyme, expandase. The extract was fractionated and characterized by colorimetric and chromatographic measurements coupled with disc-agar diffusion bioassay against an ampicillin-resistant, cephalexin...

journal_title：Biotechnology letters

authors： Thakur D,Roy MK,Bora TC

更新日期：2009-07-01 00:00:00

abstract：OBJECTIVES:To examine the synergistic antibacterial activity of violacein and silver nanoparticles (AgNPs) against ATCC bacteria, Staphylococcus aureus, Escherichia coli and two bacteria isolated from bovine mastitis. METHODS:Violacein from Chromobacterium violaceum and biogenic AgNPs from Fusarium oxysporum were eval...

journal_title：Biotechnology letters

authors： Nakazato G,Gonçalves MC,da Silva das Neves M,Kobayashi RKT,Brocchi M,Durán N

更新日期：2019-12-01 00:00:00

abstract：:Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study. To overcome this, hemangioblast studies have focused on utilizing human embryonic stem cell (hESC) derivatives but current methods are cell-line dependent...

journal_title：Biotechnology letters

authors： Gil CH,Lee JH,Seo J,Park SJ,Park Z,Kim J,Jung AR,Lee WY,Kim JS,Moon SH,Lee HT,Chung HM

更新日期：2015-06-01 00:00:00

abstract：:Lipase-catalyzed synthesis of potential multifunctional ribavirin derivatives was performed in acetone. Divinyl dicarboxylates with different chain lengths (C4, C6, C9, C10) were used as acyl donors and the reactions were catalyzed by lipase immobilized on acrylic resin from Candida antarctica (CAL-B). Ribavirin was r...

journal_title：Biotechnology letters

authors： Liu BK,Wang N,Wu Q,Xie CY,Lin XF

更新日期：2005-05-01 00:00:00

abstract：:High concentrations of 2,4,6-trinitrotoluene (TNT) and related nitroaromatic compounds are commonly found in soil and groundwater at former explosive plants. The bacterium, Raoultella terrigena strain HB, isolated from a contaminated site, converts TNT into the corresponding amino products. Radio-HPLC analysis with [(...

journal_title：Biotechnology letters

authors： Claus H,Perret N,Bausinger T,Fels G,Preuss J,König H

更新日期：2007-03-01 00:00:00

abstract：:The aim of this study was to characterize the growth of the fungus Leucoagaricus gongylophorus LEU18496, isolated from the fungus garden of the nest of leaf cutter ants Atta mexicana. The fungus garden was cultivated in an artificial laboratory nest and the fungus further grown in submerged (SmC) and solid state (SSC)...

journal_title：Biotechnology letters

authors： Maya-Yescas ME,Revah S,Le Borgne S,Valenzuela J,Palacios-González E,Terrés-Rojas E,Vigueras-Ramírez G

更新日期：2021-01-02 00:00:00

abstract：:The effects of glycerol and the oxygen transfer rate on the xylitol production rate by a xylitol dehydrogenase gene (XYL2)-disrupted mutant of Candida tropicalis were investigated. The mutant produced xylitol near the almost yield of 100% from D: -xylose using glycerol as a co-substrate for cell growth and NADPH regen...

journal_title：Biotechnology letters

authors： Ko BS,Rhee CH,Kim JH

更新日期：2006-08-01 00:00:00

abstract：:Microbes employ cell membranes for reducing exogenous stresses. Polyamino acid display on microbial cell surfaces and their effects on microbial chemical stress tolerance were examined. Growth analysis revealed that displays of polyarginine, polyaspartate and polytryptophan substantially enhanced tolerance of Escheric...

journal_title：Biotechnology letters

authors： Suzuki H,Ishii J,Kondo A,Yoshida K

更新日期：2015-02-01 00:00:00

abstract：:The basic reactions of the clostridial 1-butanol biosynthesis pathway can be regarded to be the inverted reactions of the fatty acid β-oxidation pathway. A pathway for the biosynthesis of fuels and chemicals was recently engineered by combining enzymes from both aerobic and anaerobic fatty acid β-oxidation as well as ...

journal_title：Biotechnology letters

authors： Gulevich AY,Skorokhodova AY,Sukhozhenko AV,Shakulov RS,Debabov VG

更新日期：2012-03-01 00:00:00

abstract：:The novel cancer/testis antigen gene, NY-SAR-35, is expressed exclusively in normal testis and in various histological types of tumor. However, the NY-SAR-35 gene expression is observed to be aberrant in several cancer cell lines and tissues. The analysis of methylation status of the NY-SAR-35 gene promoter in various...

journal_title：Biotechnology letters

authors： Park JH,Song MH,Lee CH,Lee MK,Park YM,Old L,Lee SY

更新日期：2011-06-01 00:00:00

abstract：OBJECTIVE:To examine the role of miR-124a in LPS-induced septic cardiac insufficiency where underlying mechanism is unclear. RESULTS:Expression of miR-124a was decreased in myocardium of LPS-induced septic cardiac dysfunction model. miR-124a antagomiR or agomiR were injected via tail vein to induce miR-124a-dysregulat...

journal_title：Biotechnology letters

authors： Diao X,Sun S

更新日期：2017-09-01 00:00:00

abstract：OBJECTIVES:To determine the effectiveness of evoglow-Pp1 as a reporter to study gene expression in bifidobacteria. To choose a strong and constitutive promoter to track fluorescently labelled bifidobacteria in environments under anaerobic conditions. RESULTS:The elongation factor P (EF-P) promoter from Bifidobacterium...

journal_title：Biotechnology letters

authors： Montenegro-Rodríguez C,Peirotén A,Sanchez-Jimenez A,Arqués JL,Landete JM

更新日期：2015-07-01 00:00:00

abstract：:Two genes, MGFc (40) and MGFc (24), encoding different E peptides of mechano-growth factor (MGF), were obtained by a four-step PCR strategy and subcloned into pRSETC and pGEX-6p-1. Recombinant MGFc(40) protein (4 mg l(-1)) was expressed and purified by affinity chromatog...

journal_title：Biotechnology letters

authors： Song L,Zhu WS,Dong JL,Li ZY,Liu XH

更新日期：2012-02-01 00:00:00

abstract：:Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...

journal_title：Biotechnology letters

authors： Zheng ZM,Xu YZ,Wang TP,Dong CQ,Yang YP,Liu DH

更新日期：2010-02-01 00:00:00