Improvement of glutathione production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach.

abstract：:Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study. To overcome this, hemangioblast studies have focused on utilizing human embryonic stem cell (hESC) derivatives but current methods are cell-line dependent...

journal_title：Biotechnology letters

authors： Gil CH,Lee JH,Seo J,Park SJ,Park Z,Kim J,Jung AR,Lee WY,Kim JS,Moon SH,Lee HT,Chung HM

更新日期：2015-06-01 00:00:00

abstract：:Clostridium spp. produce n-butanol in the acetone/butanol/ethanol process. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, the low product yield, toxicity to production strain, multiple-end products and downstream processing of alcohol mixt...

journal_title：Biotechnology letters

authors： Jurgens G,Survase S,Berezina O,Sklavounos E,Linnekoski J,Kurkijärvi A,Väkevä M,van Heiningen A,Granström T

更新日期：2012-08-01 00:00:00

abstract：:In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...

journal_title：Biotechnology letters

authors： Su M,Li Y,Ge X,Tian P

更新日期：2015-03-01 00:00:00

abstract：:An automated sequential injection analysis (SIA) system using stop-flow technique was developed to determine methanol concentration by means of the enzymatic reactions of alcohol oxidase and peroxidase. Its application as an on-line device for monitoring Pichia pastoris fermentations producing an heterologous protein ...

journal_title：Biotechnology letters

authors： Surribas A,Cos O,Montesinos JL,Valero F

更新日期：2003-11-01 00:00:00

abstract：:Pancreatic islet transplantation is used to treat diabetes mellitus that has minimal complications and avoids hypoglycemic shock. Conformal microencapsulation of pancreatic islets improves their function by blocking immunogenic molecules while protecting fragile islets. However, production of empty alginate capsules d...

journal_title：Biotechnology letters

authors： Shin S,Yoo YJ

更新日期：2013-12-01 00:00:00

abstract：:Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-08-01 00:00:00

abstract：:Among single-site mutations of L-arabinose isomerase derived from Geobacillus thermodenitrificans, two mutants were produced having the lowest and highest activities of D-tagatose production. Site-directed mutagenesis at these sites showed that the aromatic ring at amino acid 164 and the size of amino acid 475 were im...

journal_title：Biotechnology letters

authors： Oh HJ,Kim HJ,Oh DK

更新日期：2006-02-01 00:00:00

abstract：:Human granulocyte-macrophage colony stimulating factor (hGM-CSF) is used clinically to treat leucopenia typically caused by cancer chemotherapy or radiotherapy. This study used multiple strategies to obtain very high expression levels of OsrhGM-CSF (14 microg/seed) in rice endosperm. Electron micrographs of immunogold...

journal_title：Biotechnology letters

authors： Ning T,Xie T,Qiu Q,Yang W,Zhou S,Zhou L,Zheng C,Zhu Y,Yang D

更新日期：2008-09-01 00:00:00

abstract：:A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more than four times th...

journal_title：Biotechnology letters

authors： Lee SW,Jeon BY,Park DH

更新日期：2010-04-01 00:00:00

abstract：:Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...

journal_title：Biotechnology letters

authors： Zheng ZM,Xu YZ,Wang TP,Dong CQ,Yang YP,Liu DH

更新日期：2010-02-01 00:00:00

abstract：:The conventional method of transketolase (TKT) activity assay uses ribose 5-phosphate and xylulose 5-phosphate as substrates. However, a new method of TKT assay is currently required since xylulose 5-phosphate is no longer commercially available and is difficult to synthesize chemically. Although there are effective a...

journal_title：Biotechnology letters

authors： Lee JY,Cheong DE,Kim GJ

更新日期：2008-05-01 00:00:00

abstract：:Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...

journal_title：Biotechnology letters

authors： Turner KM,Pasut G,Veronese FM,Boyce A,Walsh G

更新日期：2011-03-01 00:00:00

abstract：OBJECTIVES:To evaluate the potential of enzyme cocktails produced by five filamentous fungi to supplement the industrial cellulase cocktail, Celluclast 1.5L, in order to improve the efficiency of saccharification. RESULTS:The fungi were cultivated on wheat bran and the resulting supernatants were combined with Cellucl...

journal_title：Biotechnology letters

authors： Peciulyte A,Pisano M,de Vries RP,Olsson L

更新日期：2017-09-01 00:00:00

abstract：:Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O(2) transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g ...

journal_title：Biotechnology letters

authors： Díaz-Barrera A,Silva P,Avalos R,Acevedo F

更新日期：2009-06-01 00:00:00

abstract：:The gene encoding Schwanniomyces occidentalis alpha-amylase (AMY) was introduced into the chromosomal delta sequences of an industrial strain of Saccharomyces cerevisiae. To obtain a strain suitable for commercial use, an delta-integrative cassette devoid of bacterial DNA sequences was constructed that contains the AM...

journal_title：Biotechnology letters

authors： Kang NY,Park JN,Chin JE,Lee HB,Im SY,Bai S

更新日期：2003-11-01 00:00:00

abstract：:A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...

journal_title：Biotechnology letters

authors： Ge B,Tang Z,Lin L,Ren Y,Yang Y,Qin S

更新日期：2005-06-01 00:00:00

abstract：:The baculovirus expression vector system (BEVS) is used extensively for the production of proteins from exogenous cDNAs. However, BEVS is not ideal for pharmaceutical production of glycoproteins owing to the properties of the N-glycans in the expressed products and that insect cells lack several of the enzymes require...

journal_title：Biotechnology letters

authors： Yun EY,Goo TW,Kim SW,Choi KH,Hwang JS,Kang SW,Kwon OY

更新日期：2005-07-01 00:00:00

abstract：:The addresses given for the authors of this paper were incorrect. The correct assignments of the authors are given in this erratum. ...

journal_title：Biotechnology letters

authors： Li W,Chen Y,Sheng Y,Xie Z,Tang Y

更新日期：2018-01-01 00:00:00

abstract：:Human phospholipid scramblase 1 (hPLSCR1) scrambles plasma membrane phospholipids during cell activation, blood coagulation and apoptosis. It was over-expressed in E. coli with a histidine tag and purified from the inclusion bodies (*30 mg/l culture broth) under denaturing conditions using 8 M urea. The denatured hPLS...

journal_title：Biotechnology letters

authors： Sahu SK,Gopala Krishna A,Gummadi SN

更新日期：2008-12-01 00:00:00

abstract：:Hybrids between Escherichia coli and Lactobacillus brevis were generated via protoplast fusion. Growth kinetics of five hybrid strains and E. coli were used to evaluate the butanol tolerance of the novel strains under different conditions. The hybrid strains tolerated up to 2% (v/v) butanol compared to the 1% (v/v) ma...

journal_title：Biotechnology letters

authors： Winkler J,Rehmann M,Kao KC

更新日期：2010-07-01 00:00:00

abstract：:Decrystallized chitosan was produced from shrimp shells with a low degree of crystallinity (10%) and a high anionic dye binding capacity. Raw, mixed dye wastewater from a textile factory was efficiently decolorized using decrystallized chitosan that was more efficient than using normal chitosan and activated carbon. D...

journal_title：Biotechnology letters

authors： Trung TS,Ng CH,Stevens WF

更新日期：2003-07-01 00:00:00

abstract：OBJECTIVES:Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is one of the major fungal diseases of canola. To develop resistance against this fungal disease, the chit42 from Trichoderma atroviride with chitin-binding domain and polygalacturonase-inhibiting protein 2 (PG1P2) of Phaseolus vulgaris were co-ex...

journal_title：Biotechnology letters

authors： Ziaei M,Motallebi M,Zamani MR,Panjeh NZ

更新日期：2016-06-01 00:00:00

abstract：:Washed cells (62 mg) of Kitasatospora sp. GF12 in 4 ml buffer (pH 7) catalyzed the regioselective hydroxylation of 60 mM 1,3-adamantanediol [1,3-ad(OH)(2)] to 30.9 mM 1,3,5-adamantanetriol [1,3,5-ad(OH)(3)] over 120 h at 24 °C. Glycerol at 400 mM was added to the reaction mixture to recycle the intracellular NADH/NADP...

journal_title：Biotechnology letters

authors： Mitsukura K,Yamanaka N,Yoshida T,Nagasawa T

更新日期：2012-09-01 00:00:00

abstract：:A mutant ofS. chrysomallus with a D-glucose isomerase inducible by D-glucose or its catabolites was characterized. In contrast to the wild-type strain it showed a decreased catabolite repression by D-glucose of D-xylose consumption and a constitutive pentose phosphate pathway as well. A hypothesis concerning altered i...

journal_title：Biotechnology letters

authors： Hänel F,Roth M,Neigenfind M,Gräfe U,Bormann EJ

更新日期：1987-12-01 00:00:00

abstract：:We have previously described a model to implant dissociated cells into a cylindrical, vascularized bed in vivo to promote the formation of functional cardiac muscle constructs. We now investigate the cellular organization and the ability of the constructs to generate intra-luminal pressure. Primary cardiac cells were ...

journal_title：Biotechnology letters

authors： Birla RK,Dhawan V,Dow DE,Huang YC,Brown DL

更新日期：2009-02-01 00:00:00

abstract：:The Carica papaya lipase-catalyzed acylation of benzylcarbinols with vinyl hexanoate proceeded smoothly and enantiospecifically (E > 200), affording the R-esters and leaving the S-alcohols intact. Thus, this plant lipase proved to be a promising biocatalyst for the resolution of alcohols as well as for that of carboxy...

journal_title：Biotechnology letters

authors： Miyazawa T,Houhashi M,Inoue Y,Murashima T,Yamada T

更新日期：2008-10-01 00:00:00

abstract：:The gene encoding a novel short-chain alcohol dehydrogenase in the thermophilic bacterium, Carboxydothermus hydrogenoformans, was identified and overexpressed in Escherichia coli. The enzyme was thermally stable and displayed the highest activity at 70 °C and pH 6.0. It preferred NAD(H) over NADP(H) as a cofactor and ...

journal_title：Biotechnology letters

authors： Zhou S,Zhang SC,Lai DY,Zhang SL,Chen ZM

更新日期：2013-03-01 00:00:00

abstract：OBJECTIVES:To find new metabolic engineering strategies to improve the yield of acetone in Escherichia coli. RESULTS:Results of flux balance analysis from a modified Escherichia coli genome-scale metabolic network suggested that the introduction of a non-oxidative glycolysis (NOG) pathway would improve the theoretical...

journal_title：Biotechnology letters

authors： Yang X,Yuan Q,Zheng Y,Ma H,Chen T,Zhao X

更新日期：2016-08-01 00:00:00

abstract：OBJECTIVES:To identify and characterize a novel antimicrobial peptide, catesbeianin-1. RESULTS:Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 ex...

journal_title：Biotechnology letters

authors： Xu H,Zhang Y,Feng X,Tie K,Cao Y,Han W

更新日期：2017-06-01 00:00:00

abstract：:The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW...

journal_title：Biotechnology letters

authors： Zhu L,Cui W,Fang Y,Liu Y,Gao X,Zhou Z

更新日期：2013-05-01 00:00:00