Abstract:
:The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW of 75.5 kDa. Though R. glutinis is an anamorph of Rhodosporium toruloides, the amino acid sequences of PALs them are not the same (about 74 % identity). PAL was expressed in E. coli and characterized. Its specific activity was 4.2 U mg(-1) and the k cat/K m was 1.9 × 10(4) mM(-1) s(-1), exhibiting the highest catalytic ability among the reported PALs. The genetic and biochemical information reported here should facilitate future application in industry.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Zhu L,Cui W,Fang Y,Liu Y,Gao X,Zhou Zdoi
10.1007/s10529-013-1140-7subject
Has Abstractpub_date
2013-05-01 00:00:00pages
751-6issue
5eissn
0141-5492issn
1573-6776journal_volume
35pub_type
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