Abstract:
OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. To reach reproducible architecture of co-culture spheroids, two novel 3D in vitro models with well pronounced core-shell structure from tumor spheroids and single mouse fibroblasts were developed based on this approach. The first was a combination of a RGD-peptide platform with the liquid overlay technique with further co-cultivation for 1-2 days. The second allowed co-culture spheroids to generate within polyelectrolyte microcapsules by cultivation for 2 weeks. M-3 cells (a core) and L-929 fibroblasts (a shell) were easily distinguished by confocal microscopy due to cell staining with DiO and DiI dyes, respectively. CONCLUSIONS:The 3D co-culture spheroids are proposed as a tool in tumor biology to study cell-cell interactions as well as for testing novel anticancer drugs and drug delivery vehicles.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Akasov R,Gileva A,Zaytseva-Zotova D,Burov S,Chevalot I,Guedon E,Markvicheva Edoi
10.1007/s10529-016-2218-9subject
Has Abstractpub_date
2017-01-01 00:00:00pages
45-53issue
1eissn
0141-5492issn
1573-6776pii
10.1007/s10529-016-2218-9journal_volume
39pub_type
杂志文章abstract::Fungal diseases damage crop plants and affect agricultural production. Transgenic plants have been produced by inserting antifungal genes to confer resistance against fungal pathogens. Genes of fungal cell wall-degrading enzymes, such as chitinase and glucanase, are frequently used to produce fungal-resistant transgen...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-012-0871-1
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024034508136
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pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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doi:10.1007/s10529-016-2212-2
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2005-06-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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更新日期:2004-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2099-y
更新日期:2016-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1023/b:bile.0000015424.04776.55
更新日期:2004-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-5087-6
更新日期:2004-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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更新日期:2007-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2428-9
更新日期:2017-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1400-6
更新日期:2014-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000018261.14427.e8
更新日期:2004-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000044455.42471.bf
更新日期:2004-10-01 00:00:00