Regioselective synthesis of 1,3,5-adamantanetriol from 1,3-adamantanediol using Kitasatospora cells.

abstract：:Biosurfactants could potentially outperform traditional surfactants in many applications whilst being more sustainable to source, manufacture, use and dispose of. However, currently available fermentation production methods are too inefficient to manufacture biosurfactants for these high volume markets. Foaming in an ...

journal_title：Biotechnology letters

authors： Winterburn JB,Martin PJ

更新日期：2012-02-01 00:00:00

abstract：:As more and more cell and gene therapies are being developed and with the increasing number of regulatory approvals being obtained, there is an emerging and pressing need for industrial translation. Process efficiency, associated cost drivers and regulatory requirements are issues that need to be addressed before indu...

journal_title：Biotechnology letters

authors： Moutsatsou P,Ochs J,Schmitt RH,Hewitt CJ,Hanga MP

更新日期：2019-11-01 00:00:00

abstract：:ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial...

journal_title：Biotechnology letters

authors： Shen Y,Huang H,Zhu L,Luo M,Chen D

更新日期：2012-11-01 00:00:00

abstract：:Characterization of cry gene contents can help to predict the insecticidal activities of Bacillus thuringiensis isolates and in the searching of new cry genes. PCR-Single-strand conformation polymorphism (SSCP) profiling and sequencing of the highly variable cry gene regions were used to characterize cry gene content ...

journal_title：Biotechnology letters

authors： Lin Y,Fang G,Peng K

更新日期：2007-02-01 00:00:00

abstract：OBJECTIVES:To use molecular docking and dynamic simulation to investigate the inhibitory action of chlorogenic acid (CHA) and its analogues against sortase A of Staphylococcus aureus. RESULTS:Five novel, natural inhibitors with different activities were discovered for sortase A (SrtA). The inhibition mechanism of the ...

journal_title：Biotechnology letters

authors： Bi C,Wang L,Niu X,Cai H,Zhong X,Deng X,Wang T,Wang D

更新日期：2016-08-01 00:00:00

abstract：:Antibody drug conjugates (ADCs) have emerged as a viable option in targeted delivery of highly potent cytotoxic drugs in treatment of solid tumors. At the time of writing, only two ADCs have received regulatory approval with >40 others in clinical development. The first generation ADCs suffered from a lack of specific...

journal_title：Biotechnology letters

authors： Bakhtiar R

更新日期：2016-10-01 00:00:00

abstract：OBJECTIVE:To develop recombinant factor IX (FIX) variants with augmented clotting activity. RESULTS:We generated three new variants, FIX-YKALW, FIX-ALL and FIX-LLW, expressed in SK-Hep-1 cells and characterized in vitro and in vivo. FIX-YKALW showed the highest antigen expression level among the variants (2.17 µg-mL),...

journal_title：Biotechnology letters

authors： Sousa Bomfim A,Corrêa de Freitas MC,Picanço Castro V,Abreu Soares Neto M,Pádua R,Covas DT,Sousa Russo EM

更新日期：2021-01-01 00:00:00

abstract：:Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytube...

journal_title：Biotechnology letters

authors： Komaraiah P,Reddy GV,Reddy PS,Raghavendra AS,Ramakrishna SV,Reddanna P

更新日期：2003-04-01 00:00:00

abstract：:Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocat...

journal_title：Biotechnology letters

authors： Choi OK,Choi KS,Ryu HW,Chang YK

更新日期：2003-01-01 00:00:00

abstract：:We have cloned a novel lipase gene, lip2, from Aspergillus niger and expressed it in Escherichia coli. Upon purification of the recombinant Lip2 protein, its properties were characterized. In comparison with a previously identified lipase Lip1, both enzymes are acid lipases (optimal pH <6.5), Ca(2+)-dependent and PMSF...

journal_title：Biotechnology letters

authors： Yang J,Sun J,Yan Y

更新日期：2010-07-01 00:00:00

abstract：:O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...

journal_title：Biotechnology letters

authors： Ning H,Zhang C,Yao Y,Yu D

更新日期：2010-04-01 00:00:00

abstract：:Seventeen independent transgenic rice plants with the maize anthocyanin regulatory gene Lc under control of the CaMV 35S promoter were obtained and verified by molecular identification. Ten plants showed red spikelets during early development of florets, and the degenerate florets were still red after heading. Additio...

journal_title：Biotechnology letters

authors： Li Y,Zhang T,Shen ZW,Xu Y,Li JY

更新日期：2013-01-01 00:00:00

abstract：:Our current knowledge of protein unfolding is overwhelmingly related to reversible denaturation. However, to engineer thermostable enzymes for industrial applications and medical diagnostics, it is necessary to consider irreversible denaturation processes and/or the entire quaternary structure. In this study we have u...

journal_title：Biotechnology letters

authors： Kaneko H,Minagawa H,Shimada J

更新日期：2005-11-01 00:00:00

abstract：:Ultraviolet-B (UV-B) radiation has significantly negative effect on cell survival rate (P < 0.01) and positive effect on astaxanthin accumulation (P < 0.01) of Haematococcus pluvialis. H. pluvialis accumulated 3.2 mg/g of astaxanthin when being exposed to 5 W/m(2) of UV-B for 60 min prior to 72 h of high light treatme...

journal_title：Biotechnology letters

authors： Wu Z,Chen G,Chong S,Mak NK,Chen F,Jiang Y

更新日期：2010-12-01 00:00:00

abstract：:Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. U...

journal_title：Biotechnology letters

authors： Tang X,Zheng J,Yan Q,Li Z,Li Y

更新日期：2013-06-01 00:00:00

abstract：OBJECTIVES:Marc-145 cells (monkey embryonic kidney epithelial cells) play a critical role in the biotechnology industry as certain virus host cells. To investigate the expression of enhanced green fluorescent protein (eGFP) gene as a foreign gene in Marc-145 cells, which we developed an approach of foreign gene site-sp...

journal_title：Biotechnology letters

authors： Chang Y,Shao J,Gao Y,Liu W,Gao Z,Hu Y,Chang H

更新日期：2020-08-01 00:00:00

abstract：:Glycine soja is a species of soybean that survives in adverse environments including high salt and drought conditions. We constructed a cDNA library from G. soja seedlings treated with NaCl and isolated a glutathione S-transferase gene (GsGST: GQ265911) from the library. The cDNA encoding GsGST contains an open readin...

journal_title：Biotechnology letters

authors： Ji W,Zhu Y,Li Y,Yang L,Zhao X,Cai H,Bai X

更新日期：2010-08-01 00:00:00

abstract：OBJECTIVES:Establish a method to indirectly measure evaporation in microwell-based cell culture systems and show that the proposed method allows compensating for liquid losses in fed-batch processes. RESULTS:A correlation between evaporation and the concentration of Na+ was found (R2 = 0.95) when using the 24-well-bas...

journal_title：Biotechnology letters

authors： Wiegmann V,Martinez CB,Baganz F

更新日期：2018-07-01 00:00:00

abstract：:The promoter region of the pur operon, which contains 12 genes for inosine monophosphate biosynthesis from phosphoribosylpyrophosphate, and the purA gene, encoding the adenylosuccinate synthetase, were compared among wild-type and three purine-producing Bacillus subtilis strains. A single nucleotide deletion at positi...

journal_title：Biotechnology letters

authors： Qian J,Cai X,Chu J,Zhuang Y,Zhang S

更新日期：2006-06-01 00:00:00

abstract：:In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...

journal_title：Biotechnology letters

authors： Su M,Li Y,Ge X,Tian P

更新日期：2015-03-01 00:00:00

abstract：:The Carica papaya lipase-catalyzed acylation of benzylcarbinols with vinyl hexanoate proceeded smoothly and enantiospecifically (E > 200), affording the R-esters and leaving the S-alcohols intact. Thus, this plant lipase proved to be a promising biocatalyst for the resolution of alcohols as well as for that of carboxy...

journal_title：Biotechnology letters

authors： Miyazawa T,Houhashi M,Inoue Y,Murashima T,Yamada T

更新日期：2008-10-01 00:00:00

abstract：:We have previously described a model to implant dissociated cells into a cylindrical, vascularized bed in vivo to promote the formation of functional cardiac muscle constructs. We now investigate the cellular organization and the ability of the constructs to generate intra-luminal pressure. Primary cardiac cells were ...

journal_title：Biotechnology letters

authors： Birla RK,Dhawan V,Dow DE,Huang YC,Brown DL

更新日期：2009-02-01 00:00:00

abstract：:Cellobiose is a major intermediate from cellulase hydrolysis of pretreated plant biomass. Engineering biocatalysts for direct use of cellobiose could eliminate the need for exogenous β-glucosidase. Additionally, rapid removal of cellobiose in a simultaneous saccharification and fermentation facilitates enzymatic hydro...

journal_title：Biotechnology letters

authors： Rutter C,Chen R

更新日期：2014-02-01 00:00:00

abstract：:Lentiviral vectors have been used for gene transfer into the liver but their ability to efficiently transduce quiescent hepatocytes remains controversial. Lentivirus-mediated gene transfer is more efficient in cycling cells. We determine the effect of H-IL6 in the lentiviral transduction. The lentiviral vector was use...

journal_title：Biotechnology letters

authors： Picanço-Castro V,Fontes AM,Heinz S,Tonn T,Covas DT

更新日期：2008-02-01 00:00:00

abstract：:Lipase-catalyzed synthesis of potential multifunctional ribavirin derivatives was performed in acetone. Divinyl dicarboxylates with different chain lengths (C4, C6, C9, C10) were used as acyl donors and the reactions were catalyzed by lipase immobilized on acrylic resin from Candida antarctica (CAL-B). Ribavirin was r...

journal_title：Biotechnology letters

authors： Liu BK,Wang N,Wu Q,Xie CY,Lin XF

更新日期：2005-05-01 00:00:00

abstract：:Extracts of Salvia species are used in traditional medicine to treat various diseases. The economic importance of this genus has increased in recent years due to evidence that some of its secondary metabolites have valuable pharmaceutical and nutraceutical properties.The bioactivity of sage extracts is mainly due to t...

journal_title：Biotechnology letters

authors： Marchev A,Haas C,Schulz S,Georgiev V,Steingroewer J,Bley T,Pavlov A

更新日期：2014-02-01 00:00:00

abstract：:Some recombinant proteins expressed by baculovirus expression vector systems (BEVS) aggregate because the BEVS can produce large amounts of protein late during infection, when post-translational modification and protein quality control mechanisms are inactive. For expression during earlier stages than that driven by t...

journal_title：Biotechnology letters

authors： Ishiyama S,Ikeda M

更新日期：2010-11-01 00:00:00

abstract：:Continuous production of fructooligosaccharides (FOS) by Aureobasidium pullulans immobilized on calcium alginate beads with a packed bed was investigated at a plant scale reactor. Optimum conditions were with 770 g sucrose/l, being fed at 200 l/h at 50°C which gave a productivity of 180 g FOS/l h. Initial activity was...

journal_title：Biotechnology letters

authors： Jung KH,Bang SH,Oh TK,Park HJ

更新日期：2011-08-01 00:00:00

abstract：:Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have...

journal_title：Biotechnology letters

authors： Lee HH,Lee SJ,Kim S,Jeong S,Na M,Lee DM,Cheon YP,Lee KH,Choi I,Chun T

更新日期：2012-07-01 00:00:00

abstract：OBJECTIVES:The action modes of an endocellulase, EGA, and its domains (CD9 and CBM3) during enzymatic treatment of cotton fabrics were investigated. RESULTS:EGA, CD9 and CBM3 had the binding capacity to cellulose substrates, of which the filter paper was the substrate with the strongest binding capacity. Analyses of s...

journal_title：Biotechnology letters

authors： Yu M,Qiu Y,Chen W,Zhao F,Shao J

更新日期：2015-08-01 00:00:00