Rational design of thermostable lactate oxidase by analyzing quaternary structure and prevention of deamidation.

abstract：:Fifty-two fungal isolates obtained from seawater were screened for anti-nematode activity. One isolate, an Acremonium sp., exhibited the highest activity against the pinewood nematode, Bursaphelenchus xylophilus. When it was grown at pH 7.0 and 24-26 °C, nematicidal metabolites were produced. These were heat-stable, w...

journal_title：Biotechnology letters

authors： Meng Q,Shi X,Meng F,Feng X,Sun J

更新日期：2012-10-01 00:00:00

abstract：:Of several types of chemical surfactants and biosurfactants, only the culture broth of Bacillus subtilis C1 containing surfactin at 10 mg l(-1) completely inhibited the growth of Microcystis aeruginosa, a bloom-forming cyanobacterium in highly eutrophic lakes. The broth with 10 mg surfactin l(-1) also removed 85% of t...

journal_title：Biotechnology letters

authors： Ahn CY,Joung SH,Jeon JW,Kim HS,Yoon BD,Oh HM

更新日期：2003-07-01 00:00:00

abstract：:A successful nerve regeneration process was achieved with nerve repair tubes made up of 1-ethyl-3(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) cross-linked carboxymethyl chitosan (CM-chitosan) with improved biodegradability. Chitosan has a very slow degradation rate, while the EDC cross-linked CM-chitosan t...

journal_title：Biotechnology letters

authors： Wang G,Lu G,Ao Q,Gong Y,Zhang X

更新日期：2010-01-01 00:00:00

abstract：:A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degrees C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium d...

journal_title：Biotechnology letters

authors： Theppakorn T,Kanasawud P,Halling PJ

更新日期：2004-01-01 00:00:00

abstract：:The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....

journal_title：Biotechnology letters

authors： Deak PM,Lutz-Wahl S,Bothe H,Fischer L

更新日期：2003-03-01 00:00:00

abstract：:The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respective...

journal_title：Biotechnology letters

authors： van der Valk AM,Howbrook DN,O'Shaughnessy MC,Sarker DK,Baker SC,Louwrier A,Lloyd AW

更新日期：2003-08-01 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fu...

journal_title：Biotechnology letters

authors： Jahns AC,Rehm BH

更新日期：2015-04-01 00:00:00

abstract：OBJECTIVE:The purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood. RESULTS:After incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be det...

journal_title：Biotechnology letters

authors： Hu L,Chen X,Chen M,Fang J,Nie J,Dai H

更新日期：2021-01-01 00:00:00

abstract：:Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...

journal_title：Biotechnology letters

authors： Conn S,Zhang W,Franco C

更新日期：2003-06-01 00:00:00

abstract：:Continuous production of fructooligosaccharides (FOS) by Aureobasidium pullulans immobilized on calcium alginate beads with a packed bed was investigated at a plant scale reactor. Optimum conditions were with 770 g sucrose/l, being fed at 200 l/h at 50°C which gave a productivity of 180 g FOS/l h. Initial activity was...

journal_title：Biotechnology letters

authors： Jung KH,Bang SH,Oh TK,Park HJ

更新日期：2011-08-01 00:00:00

abstract：:Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...

journal_title：Biotechnology letters

authors： Minkner R,Park EY

更新日期：2018-04-01 00:00:00

abstract：:The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix o...

journal_title：Biotechnology letters

authors： Camperi SA,Iannucci NB,Albanesi GJ,Oggero Eberhardt M,Etcheverrigaray M,Messeguer A,Albericio F,Cascone O

更新日期：2003-09-01 00:00:00

abstract：:Fusion expression is a common practice for recombinant protein production. Some fusion tags confer solubility on the target protein whereas others provide affinity handles that facilitate purification. However, the tag usually needs to be removed from the final product, which involves using expensive proteases or haza...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-05-01 00:00:00

abstract：:Fungal diseases damage crop plants and affect agricultural production. Transgenic plants have been produced by inserting antifungal genes to confer resistance against fungal pathogens. Genes of fungal cell wall-degrading enzymes, such as chitinase and glucanase, are frequently used to produce fungal-resistant transgen...

journal_title：Biotechnology letters

authors： Ceasar SA,Ignacimuthu S

更新日期：2012-06-01 00:00:00

abstract：OBJECTIVES:To identify and characterize a novel antimicrobial peptide, catesbeianin-1. RESULTS:Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 ex...

journal_title：Biotechnology letters

authors： Xu H,Zhang Y,Feng X,Tie K,Cao Y,Han W

更新日期：2017-06-01 00:00:00

abstract：:Ochrobactrum anthropi cleaved the delta-(D-alpha-aminoadipoyl)-side chain from delta-(D-alpha-aminoadipoyl)-7-amino-4-methylcoumarin, a beta-lactamase-resistant cephalosporin C analogue. In whole cell conversions up to 1 nkat g(-1) dry cell wt were achieved. O. anthropi possesses also gamma-D-glutamyltranspeptidase ac...

journal_title：Biotechnology letters

authors： Patett F,Fischer L

更新日期：2005-12-01 00:00:00

abstract：OBJECTIVES:To investigate antioxidative and biotransformation enzyme responses in Mucor hiemalis towards cyanotoxins considering its use in mycoremediation applications. RESULTS:Catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) in M. hiemalis maintained their activities at all tested microcy...

journal_title：Biotechnology letters

authors： Balsano E,Esterhuizen-Londt M,Hoque E,Lima SP

更新日期：2017-08-01 00:00:00

abstract：OBJECTIVES:To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS:Co60 gamma rays reduced viability, raised the expression of ...

journal_title：Biotechnology letters

authors： Soltani B,Bodaghabadi N,Mahpour G,Ghaemi N,Sadeghizadeh M

更新日期：2016-12-01 00:00:00

abstract：:A novel glucose-sensing molecule was created based on galactose/glucose-binding protein (GGBP). GGBP mutants at Asp14, a residue interacting with the 4th hydroxyl group of the sugar molecule, were constructed by mutagenesis to improve the ligand specificity of GGBP. The autofluorescence-based analysis of the binding a...

journal_title：Biotechnology letters

authors： Sakaguchi-Mikami A,Taneoka A,Yamoto R,Ferri S,Sode K

更新日期：2008-08-01 00:00:00

abstract：:We truncated the VP2 protein of infectious bursal disease virus into five fragments: V1-5. All fragments were displayed on the inner membrane of the Escherichia coli periplasm. After disruption of the outer membrane, spheroplasts that had anchored with the VP2 fragment were incubated with an anti-VP2 polyclonal antibo...

journal_title：Biotechnology letters

authors： Guo M,Xu LM,Zhou B,Yin JC,Ye XL,Ren GP,Li DS

更新日期：2014-03-01 00:00:00

abstract：OBJECTIVE:This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. RESULTS:This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we f...

journal_title：Biotechnology letters

authors： Huang X,Zheng M,Yi Y,Patel A,Song Z,Li Y

更新日期：2020-11-01 00:00:00

abstract：:Kluyveromyces marxianus was grown in submerged culture in a complex medium with several potential inducers of lipolytic activity (triacylglycerols, fatty acids). The highest extracellular lipolytic enzyme production (about 80 U ml(-1) in 3 d) was obtained when the medium was supplemented with 2 g urea l(-1) plus 5 g t...

journal_title：Biotechnology letters

authors： Deive FJ,Costas M,Longo MA

更新日期：2003-09-01 00:00:00

abstract：OBJECTIVE:A bio-based process is appealing for purification of L-lactic acid, the major enantiomer of polylactic acid syrup, generated by thermochemical processes at the end of life of PLA-based plastics, from its chiral impurity, D-lactic acid, before reuse. RESULTS:Polylactic acid (PLA), a renewable alternative to p...

journal_title：Biotechnology letters

authors： Chauliac D,Pullammanappallil PC,Ingram LO,Shanmugam KT

更新日期：2015-12-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：:Cell-based therapeutics are promising routes for the regeneration of damaged cells and organs. The recovery of cells cultured in vitro for such applications requires the use of proteolytic enzymes which deteriorate its property by disruption of cell-cell and cell-matrix interactions. Intact cell sheets can be retrieve...

journal_title：Biotechnology letters

authors： Mukundan LM,Nirmal R,Thomas LV,Sajeev US,Nair PD

更新日期：2011-10-01 00:00:00

abstract：OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. ...

journal_title：Biotechnology letters

authors： Akasov R,Gileva A,Zaytseva-Zotova D,Burov S,Chevalot I,Guedon E,Markvicheva E

更新日期：2017-01-01 00:00:00

abstract：OBJECTIVE:To develop recombinant factor IX (FIX) variants with augmented clotting activity. RESULTS:We generated three new variants, FIX-YKALW, FIX-ALL and FIX-LLW, expressed in SK-Hep-1 cells and characterized in vitro and in vivo. FIX-YKALW showed the highest antigen expression level among the variants (2.17 µg-mL),...

journal_title：Biotechnology letters

authors： Sousa Bomfim A,Corrêa de Freitas MC,Picanço Castro V,Abreu Soares Neto M,Pádua R,Covas DT,Sousa Russo EM

更新日期：2021-01-01 00:00:00

abstract：:An optimal pooling system, called Accurate and Fast Target Screening, has been developed for high-throughput identifying the rare marker-free transformants. This system can identify targets between 10- and 100-fold more efficiently than analysis of individual samples. By calculating the efficiency for different propor...

journal_title：Biotechnology letters

authors： Zhang J,Xiao Q,Li K,Chen M,Chang J,Luo L,Li Y,Liu Y,Shewry PR,He G

更新日期：2006-10-01 00:00:00

abstract：:A new irreversible esterification method for carboxylic acids catalyzed by a lipase from Candida antarctica (Novozyme 435) in organic solvents has been developed. The water produced during the process is chemically destroyed by a corresponding ester of acetoacetate, which acts as a sacrificial substrate in this reacti...

journal_title：Biotechnology letters

authors： Jeromin GE,Zoor A

更新日期：2008-05-01 00:00:00

abstract：:Ammonium is a substrate of the anaerobic ammonium oxidation (Anammox) process but it has been suggested as a substrate-inhibitor because of the action of its unionized form, free ammonia. High pH of the medium is also an important limiting factor of the Anammox bacteria. Both effects are difficult to discriminate. In ...

journal_title：Biotechnology letters

authors： Puyol D,Carvajal-Arroyo JM,Li GB,Dougless A,Fuentes-Velasco M,Sierra-Alvarez R,Field JA

更新日期：2014-10-01 00:00:00