High pH (and not free ammonia) is responsible for Anammox inhibition in mildly alkaline solutions with excess of ammonium.

abstract：OBJECTIVE:A bio-based process is appealing for purification of L-lactic acid, the major enantiomer of polylactic acid syrup, generated by thermochemical processes at the end of life of PLA-based plastics, from its chiral impurity, D-lactic acid, before reuse. RESULTS:Polylactic acid (PLA), a renewable alternative to p...

journal_title：Biotechnology letters

authors： Chauliac D,Pullammanappallil PC,Ingram LO,Shanmugam KT

更新日期：2015-12-01 00:00:00

abstract：OBJECTIVE:To develop a mouse artificial chromosome (MAC) carrying the mouse Xist gene (X-inactive specific transcript; Xist-MAC) as a systematic in vitro approach for investigating Xist RNA-mediated chromosome inactivation. RESULTS:Ectopic expression of the Xist gene in CHO cells led to the accumulation of Xist RNA in...

journal_title：Biotechnology letters

authors： Inaoka D,Sunamura N,Ohira T,Nakayama Y,Kugoh H

更新日期：2020-05-01 00:00:00

abstract：OBJECTIVES:To improve the thermostability and catalytic property of a mesophilic 1,3-1,4-β-glucanase by combinational mutagenesis and to test its effect in congress mashing. RESULTS:A mutant β-glucanase (rE-BglTO) constructed by combinational mutagenesis showed a 25 °C increase in optimal temperature (to 70 °C) a 19.5...

journal_title：Biotechnology letters

authors： Niu C,Zhu L,Hill A,Alex Speers R,Li Q

更新日期：2017-01-01 00:00:00

abstract：:Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6)...

journal_title：Biotechnology letters

authors： Hao G,Chen H,Du K,Huang X,Song Y,Gu Z,Wang L,Zhang H,Chen W,Chen YQ

更新日期：2014-09-01 00:00:00

abstract：:UV-induced DNA damage plays a key role in the etiology of certain diseases. The ability of blueberry anthocyanins and anthocyanidins (BA) to protect cellular DNA from UV-induced damage was investigated. BA were extracted by water (BAW), ethanol (BAE) or methanol (BAM). These extracts partially restored proliferation o...

journal_title：Biotechnology letters

authors： Liu W,Lu X,He G,Gao X,Li M,Wu J,Li Z,Wu J,Wang J,Luo C

更新日期：2013-04-01 00:00:00

abstract：OBJECTIVES:The potential of CRISPR/Cas9 gene editing to repress CyHV-3 was tested in vitro. RESULTS:By targeting two basic target genes necessary for the early transcription of CyHV-3, we show that virus transcription and particle release were significantly decreased by CRISPR/Cas9, as measured by quantitative real-ti...

journal_title：Biotechnology letters

authors： Zhao Y,Wang T,Yu Z,Wang H,Liu B,Wu C,Teng CB

更新日期：2016-04-01 00:00:00

abstract：:The recent progress in knowledge on biochemical properties and functions of phospholipases A(2) in plants paved the way for approving the suitability of these enzymes for commercial use now. The secreted phospholipases A(2), representing one type of phospholipases A(2) occurring in plants, show distinct differences in...

journal_title：Biotechnology letters

authors： Mansfeld J

更新日期：2009-09-01 00:00:00

abstract：:A novel lipase gene TAlipC was isolated from Trichosporon asahii MSR54 and functionally expressed in Pichia pastoris. The protein was His-tagged and purified to homogeneity by affinity chromatography. Sequence comparison revealed a high homology with lipases from Cryptococcus sp. It had a GX type oxyanion hole and a G...

journal_title：Biotechnology letters

authors： Kumari A,Gupta R

更新日期：2015-01-01 00:00:00

abstract：:Of several types of chemical surfactants and biosurfactants, only the culture broth of Bacillus subtilis C1 containing surfactin at 10 mg l(-1) completely inhibited the growth of Microcystis aeruginosa, a bloom-forming cyanobacterium in highly eutrophic lakes. The broth with 10 mg surfactin l(-1) also removed 85% of t...

journal_title：Biotechnology letters

authors： Ahn CY,Joung SH,Jeon JW,Kim HS,Yoon BD,Oh HM

更新日期：2003-07-01 00:00:00

abstract：:The proteome of rumen epithelial tissue was analysed by SDS-PAGE coupled with LC-MS/MS. 813 non-redundant proteins were identified of which 7.4 % featured membrane-spanning domains and 15.4 % harboured a signal peptide. According to the gene ontology annotation, the most abundant proteins exhibited binding activities ...

journal_title：Biotechnology letters

authors： Yang Y,Wang J,Yuan T,Bu D,Yang J,Sun P

更新日期：2013-11-01 00:00:00

abstract：:An extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25°C. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. EstK was purified to homogeneit...

journal_title：Biotechnology letters

authors： Hong S,Lee C,Jang SH

更新日期：2012-06-01 00:00:00

abstract：:Ochratoxin A is a mycotoxin produced by several Aspergillus and some Penicillium species which may be present in food and feed products. It can be enzymatically hydrolyzed into ochratoxin alpha and L: -beta-phenylalanine, thereby decreasing its toxicity. The ochratoxin A degradation capacity of Aspergillus niger is we...

journal_title：Biotechnology letters

authors： Abrunhosa L,Venâncio A

更新日期：2007-12-01 00:00:00

abstract：:Long-term cell recycle fermentations of Candida tropicalis were performed over 14 rounds of fermentation. The average xylitol concentrations, fermentation times, volumetric productivities and product yields for 14 rounds were 105 g l(-1), 333 h, 4.4 g l(-1) h(-1) and 78%, respectively, in complex medium; and 110 g l(-...

journal_title：Biotechnology letters

authors： Kim TB,Lee YJ,Kim P,Kim CS,Oh DK

更新日期：2004-04-01 00:00:00

abstract：:In this study, 3D culture system for human adipose-derived stem cell (hASC) using a BioLevitator as the bioreactor for microcarrier-based cultures was established. During the culturing period, hASCs preferred to grow in crevices between microcarriers and a high viability was maintained even when reaching confluency. A...

journal_title：Biotechnology letters

authors： Lin CY,Huang CH,Wu YK,Cheng NC,Yu J

更新日期：2014-07-01 00:00:00

abstract：:The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection wa...

journal_title：Biotechnology letters

authors： Chun BH,Lee YK,Chung N

更新日期：2012-10-01 00:00:00

abstract：OBJECTS:To explore the roles of growth factor receptor-bound protein 14 (GRB14) in colorectal cancer (CRC) and its correlation with clinicopathological characteristics and prognosis of CRC patients. RESULTS:GRB14 was localized in the cytoplasm of CRC and benign glandular epithelium cells, showing higher levels in CRC ...

journal_title：Biotechnology letters

authors： Yang P,Wei J,Li W,He F,Zeng S,Zhang T,Sun Z,Cao J

更新日期：2016-06-01 00:00:00

abstract：:O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...

journal_title：Biotechnology letters

authors： Ning H,Zhang C,Yao Y,Yu D

更新日期：2010-04-01 00:00:00

abstract：OBJECTIVE:The growth characteristics and intracellular hemin concentrations of Lactococcus lactis grown under different culture pH and aeration conditions were examined to investigate the effect of culture pH on the respiration efficiency of L. lactis NZ9000 (pZN8148). RESULTS:Cell biomass and biomass yield of L. lact...

journal_title：Biotechnology letters

authors： Shi W,Li Y,Gao X,Fu R

更新日期：2016-03-01 00:00:00

abstract：:A prospective clinical parametric study comprising women afflicted by breast cancer and otherwise healthy participants was undertaken. The mean plasmatic concentration of putative leucine amino peptidase and nucleoside diphosphate phosphotransferase enzymatic complex in breast cancer cases was significantly elevated [...

journal_title：Biotechnology letters

authors： Pulido-Cejudo G,Miranda H,El Abdaimi K,Wang C,Kar B,Medina Acevedo J,Cárdenas JM,Sarti Gutiérrez E,Pérez Palacios G

更新日期：2004-09-01 00:00:00

abstract：:Environmental factors, such as drought, salinity, extreme temperature, ozone poisoning, metal toxicity etc., significantly affect crops. To study these factors and to design a possible remedy, biological experimental data concerning these crops requires the quantification of gene expression and comparative analyses at...

journal_title：Biotechnology letters

authors： Gul A,Ahad A,Akhtar S,Ahmad Z,Rashid B,Husnain T

更新日期：2016-04-01 00:00:00

abstract：:The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene su...

journal_title：Biotechnology letters

authors： Lim HA,Raku T,Tokiwa Y

更新日期：2005-04-01 00:00:00

abstract：:Three chloroplast genes, chlL, chlN and chlB, encoding the light-independent protochlorophyllide oxidoreductase (LIPOR) in Chlorella protothecoides CS-41 growing either photoautotrophically, mixotrophically or heterotrophically, were all transcribed constitutively independent of illumination and presence of glucose. S...

journal_title：Biotechnology letters

authors： Shi C,Shi X

更新日期：2006-02-01 00:00:00

abstract：OBJECTIVES:To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions. RESULTS:The maximal ethanol c...

journal_title：Biotechnology letters

authors： Techaparin A,Thanonkeo P,Klanrit P

更新日期：2017-10-01 00:00:00

abstract：:We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic v...

journal_title：Biotechnology letters

authors： Hara KY,Suzuki R,Suzuki T,Yoshida M,Kino K

更新日期：2011-06-01 00:00:00

abstract：:A polysaccharide from the traditional Chinese medicinal herb, Bletilla striata (Thunb.) Reichb. f., was isolated, purified and characterized. It induced the proliferation of human umbilical vascular endothelial cells and the expression of vascular endothelial growth factor up to 156% and 147% of control after 72 h, re...

journal_title：Biotechnology letters

authors： Wang C,Sun J,Luo Y,Xue W,Diao H,Dong L,Chen J,Zhang J

更新日期：2006-04-01 00:00:00

abstract：:The genetics governing the morphological switch from round or ovoid cells to filamentous growth in Saccharomyces cerevisiae has received significant interest in relation to sensing and signaling pathways as well as the control of cell processes including budding, elongation and adhesion. Little is known about the envi...

journal_title：Biotechnology letters

authors： Ceccato-Antonini SR

更新日期：2008-07-01 00:00:00

abstract：:Fourteen strains of Bacillus thuringiensis collected from both larvae showing disease symptoms and soil samples in northwest Argentina were characterized by insecticidal activity against Spodoptera frugiperda. First instar larvae and protein profile SDS-PAGE analysis of whole cell proteins not only allowed the differe...

journal_title：Biotechnology letters

authors： Alvarez A,Pera LM,Loto F,Virla EG,Baigori MD

更新日期：2009-01-01 00:00:00

abstract：OBJECTIVES:To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. RESULTS:HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung canc...

journal_title：Biotechnology letters

authors： Zhang Y,Yang L,Ling C,Heng W

更新日期：2018-04-01 00:00:00

abstract：:Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the m...

journal_title：Biotechnology letters

authors： Bhosale P,Serban B,Bernstein PS

更新日期：2006-09-01 00:00:00

abstract：:Unexpected inversion of the 3':5'-regioselectivity was observed in the enzymatic methacryloylation, crotonylation and cinnamoylation of floxuridine (1.5:1, 2.3:1 and 4.4:1, respectively), where Thermomyces lanuginosus lipase preferentially catalyzed the acylation of 3'-hydroxyl rather than that of 5'-hydroxyl group. T...

journal_title：Biotechnology letters

authors： Li N,Zong MH,Ma D

更新日期：2009-08-01 00:00:00