Abstract:
:Fourteen strains of Bacillus thuringiensis collected from both larvae showing disease symptoms and soil samples in northwest Argentina were characterized by insecticidal activity against Spodoptera frugiperda. First instar larvae and protein profile SDS-PAGE analysis of whole cell proteins not only allowed the differentiation of native Bacillus thuringiensis but also revealed the possibility of applying protein profile analysis in classification of toxicity patterns. Cluster analysis showed that there were two main groups. Interestingly, one of them only contained the most pathogenic native strains. The biomass-bound protease activity of native pathogenic isolates and the reference strain Bt 4D1 is also reported.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Alvarez A,Pera LM,Loto F,Virla EG,Baigori MDdoi
10.1007/s10529-008-9841-zsubject
Has Abstractpub_date
2009-01-01 00:00:00pages
77-82issue
1eissn
0141-5492issn
1573-6776journal_volume
31pub_type
杂志文章abstract::Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02767-2
更新日期:2020-03-01 00:00:00
abstract::Seeds play essential roles in plant life cycle and germination is a complex process which is associated with different phases of water imbibition. Upon imbibition, seeds begin utilization of storage substances coupled with metabolic activity and biosynthesis of new proteins. Regeneration of organelles and emergence of...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-017-2393-3
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVE:To construct a highly expressed and active MEK1R4F (a constitutively active form of mitogen-activated protein kinase kinase 1) by fusion of soluble adenylate kinase (Adk) tag, resulting in Adk-MEK1R4F protein suitable for preparation of phosphorylated ERK. RESULTS:We fused the Adk to the N-terminus of MEK1R4...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2385-3
更新日期:2017-10-01 00:00:00
abstract::To control the genetic quality during the whole process of tissue culture of the traditional Chinese medicinal plant, Saussurea involucrate Kar. et Kir., DNA polymorphisms and genetic variations were investigated using randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. The gene...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9984-6
更新日期:2009-08-01 00:00:00
abstract::Dodecanol (1% v/v) and dodecanoic acid (1% w/v) inhibited growth of Yarrowia lipolytica in complex media supplemented with glucose but dodecanedioic acid (1% w/v) was not toxic. Dodecanol-tolerant strains were prepared from the wild type strain H222 as well as the acyl-CoA oxidase deleted (deltaPOX2, POX3, POX5) strai...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000025890.97067.98
更新日期:2004-05-01 00:00:00
abstract::Brevibacterium epidermis DSM 20659 is a halotolerant Gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. The present study revealed that B. epidermis is equipped with at least one transport system for ectoine, with a maximal transport velocity of 15.7 +/-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9149-9
更新日期:2006-11-01 00:00:00
abstract::The gene encoding a novel short-chain alcohol dehydrogenase in the thermophilic bacterium, Carboxydothermus hydrogenoformans, was identified and overexpressed in Escherichia coli. The enzyme was thermally stable and displayed the highest activity at 70 °C and pH 6.0. It preferred NAD(H) over NADP(H) as a cofactor and ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1082-5
更新日期:2013-03-01 00:00:00
abstract:OBJECTIVE:Silk sericin is a natural polymer with potential utility in biomedical and biotechnological applications. Recombinantly expressed sericin ensures a source of pure protein with no contamination and with multiple properties when expressed as a fusion protein. Hence, the present paper aims to recombinantly expre...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02911-3
更新日期:2020-09-01 00:00:00
abstract::An efficient synthesis of polymerizable 3'- and 5'-O-acyl-nucleoside derivatives has been developed from inosine and 2'-deoxyuridine by enzyme-catalyzed regioselective acylation with divinyl dicarboxylates. In acetone, Lipozyme (immobilized lipase from Mucor miehei) gave 5'-O-acyl-nucleoside products, and PPL (lipase ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000030050.20353.a6
更新日期:2004-06-01 00:00:00
abstract:OBJECTIVES:To prepare (R)-phenyl-1,2-ethanediol ((R)-PED) with high enantiomeric excess (ee p) and yield from racemic styrene oxide (rac-SO) at high concentration by bi-enzymatic catalysis. RESULTS:The bi-enzymatic catalysis was designed for enantioconvergent hydrolysis of rac-SO by a pair of novel epoxide hydrolases ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2433-z
更新日期:2017-12-01 00:00:00
abstract::Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1631-1
更新日期:2014-12-01 00:00:00
abstract::Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8655-5
更新日期:2005-08-01 00:00:00
abstract:OBJECTIVES:To optimize the production of active inclusion bodies (IBs) containing human D-amino acid oxidase (hDAAO) in Escherichia coli. RESULTS:The optimized initial codon region combined with the coexpressed rare tRNAs, fusion of each of the N-terminal partners including cellulose-binding module, thioredoxin, gluta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2413-3
更新日期:2017-11-01 00:00:00
abstract::Dibutyl phthalate (DBP) was more efficiently degraded by cutinase compared to yeast esterase; i.e. almost 80% of initial DBP (500 mg l(-1)) was decomposed within 7.5 h, and nearly 50% of the degraded DBP disappeared within the initial 30 min. The toxicity of the final DBP degradation products were investigated using v...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3631-7
更新日期:2005-05-01 00:00:00
abstract::Error-prone PCR was used to create more thermoactive and/or thermostable variants of thermoalkalophilic lipases. A variant of the α6 helix (lid domain), with an 189E to V substitution at residue 189, lost its thermostability but exhibited higher activity than that of its wild-type predecessor (r03Lip). Site-saturation...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0635-3
更新日期:2011-09-01 00:00:00
abstract::A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of gamma-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred ta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-0003-x
更新日期:2006-04-01 00:00:00
abstract::The biotin-binding capacity in the wells of a streptavidin-coated PCR plate were quantified by means of a fluorescence intensity assay utilising biotin-labelled fluorescein and a colorimetric assay using biotin-labelled alkaline phosphatase. The biotin binding capacities were determined to be 59 and 58 pmol respective...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024951322955
更新日期:2003-08-01 00:00:00
abstract::Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PA...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0924-5
更新日期:2012-08-01 00:00:00
abstract::Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0592-x
更新日期:2011-08-01 00:00:00
abstract:OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2218-9
更新日期:2017-01-01 00:00:00
abstract::Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2516-5
更新日期:2018-04-01 00:00:00
abstract:OBJECTIVES:For the stress from fermenters, downstream processing equipment, and wastewater treatment to be alleviated, lowering salt-dependence in the ectoine synthesis process is of great significance in the moderately halotolerant Halomonas hydrothermalis Y2. RESULTS:In H. hydrothermalis Y2, the σ70- and σ38-control...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-021-03084-3
更新日期:2021-01-27 00:00:00
abstract::The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1140-7
更新日期:2013-05-01 00:00:00
abstract::Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the cont...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0985-5
更新日期:2012-10-01 00:00:00
abstract::A gene encoding a gamma-butyrolactone autoregulator receptor was cloned in to E. coli from Streptomyces ambofaciens producing spiramycin, a macrolide antibiotic used in both veterinary medicine and human medicine. A 714-bp intact receptor gene (saaR) was obtained by PCR and genomic Southern hybridization with the 100-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9613-1
更新日期:2008-05-01 00:00:00
abstract::In order to treat the kitchen wastes and produce hydrogen, anaerobic fermentation technology was used in this experiment. The results showed that the fermentation type changed from mixed acid fermentation to ethanol fermentation in a continuous stirred tank reactor (CSTR) 22 days after start-up. The maximum efficiency...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0024-3
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVES:To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. RESULTS:HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung canc...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2512-9
更新日期:2018-04-01 00:00:00
abstract::A novel two-step fermentation process was developed to enhance arachidonic acid (ARA) production by Mortierella alpina ME-1 in a 5 l fermentor. Agitation speed and aeration rate were adjusted from 180 to 40 rpm and from 0.6 to 1 vvm, respectively, after 5 days cultivation, to decrease physical damage to the mycelia an...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9661-1
更新日期:2008-06-01 00:00:00
abstract::Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0741-2
更新日期:2012-01-01 00:00:00
abstract::The cDNA of the starch-branching enzyme I gene (IbSBEI) in the sweet potato (Ipomoea batatas) has been cloned and sequenced. The IbSBEI amino acid sequence was 81% identical to that of potato StSBEI. DNA gel-blot analyses demonstrated that at least two copies of IbSBEI are present in the sweet potato genome. IbSBEI wa...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9083-x
更新日期:2006-08-01 00:00:00