Controllable regioselective enzymatic synthesis of polymerizable 5'-O-vinyl- and 3'-O-vinyl-nucleoside analogues in acetone.

abstract：:Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylami...

journal_title：Biotechnology letters

authors： Huh YS,Hong YK,Hong WH,Chang HN

更新日期：2004-10-01 00:00:00

abstract：:Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...

journal_title：Biotechnology letters

authors： Zheng ZM,Xu YZ,Wang TP,Dong CQ,Yang YP,Liu DH

更新日期：2010-02-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：OBJECTIVE:The purpose of the article is to establish a quick enrichment and detection method using immunomagnetic beads and flow cytometry to analyze circulating tumor cells (CTCs) in the peripheral blood. RESULTS:After incubation with CD326-PE and CD45-APC antibodies, more than 60% MCF7 cells in M-Buffer could be det...

journal_title：Biotechnology letters

authors： Hu L,Chen X,Chen M,Fang J,Nie J,Dai H

更新日期：2021-01-01 00:00:00

abstract：:Constant medium feeding rate and intermittent fed-batch fermentation strategies were investigated aiming to increase the yields of γ-decalactone production by Yarrowia lipolytica, using methyl ricinoleate as substrate and ricinoleic acid source. The accumulation of another compound, 3-hydroxy-γ-decalactone, was also a...

journal_title：Biotechnology letters

authors： Gomes N,Teixeira JA,Belo I

更新日期：2012-04-01 00:00:00

abstract：:The baculovirus expression vector system (BEVS) is used extensively for the production of proteins from exogenous cDNAs. However, BEVS is not ideal for pharmaceutical production of glycoproteins owing to the properties of the N-glycans in the expressed products and that insect cells lack several of the enzymes require...

journal_title：Biotechnology letters

authors： Yun EY,Goo TW,Kim SW,Choi KH,Hwang JS,Kang SW,Kwon OY

更新日期：2005-07-01 00:00:00

abstract：OBJECTIVE:To develop recombinant factor IX (FIX) variants with augmented clotting activity. RESULTS:We generated three new variants, FIX-YKALW, FIX-ALL and FIX-LLW, expressed in SK-Hep-1 cells and characterized in vitro and in vivo. FIX-YKALW showed the highest antigen expression level among the variants (2.17 µg-mL),...

journal_title：Biotechnology letters

authors： Sousa Bomfim A,Corrêa de Freitas MC,Picanço Castro V,Abreu Soares Neto M,Pádua R,Covas DT,Sousa Russo EM

更新日期：2021-01-01 00:00:00

abstract：:An integrative vector was constructed for inserting heterologous genes within a non-functional open reading frame (ORF) on the chromosome of Streptococcus thermophilus. The vector, pINTRS, contained a temperature sensitive origin of replication and an erythromycin resistance gene for initial selection in S. thermophil...

journal_title：Biotechnology letters

authors： Renye JA Jr,Somkuti GA

更新日期：2009-05-01 00:00:00

abstract：:The addresses given for the authors of this paper were incorrect. The correct assignments of the authors are given in this erratum. ...

journal_title：Biotechnology letters

authors： Li W,Chen Y,Sheng Y,Xie Z,Tang Y

更新日期：2018-01-01 00:00:00

abstract：:Deer antlers are the only mammalian appendages capable of repeated rounds of regeneration. Every year, deer antlers are shed and regrown from blastema into large branched structures of cartilage and bone. Little is known about the genes involved in antler development particularly during the later stages of ossificatio...

journal_title：Biotechnology letters

authors： Yao B,Zhao Y,Zhang H,Zhang M,Liu M,Liu H,Li J

更新日期：2012-05-01 00:00:00

abstract：:Flavonoids have been proposed to play diverse roles in plant growth and development, including defense, symbiosis, pollen development and male fertility, polar auxin transport, and protection against ultraviolet radiation. Recently, a new role in cell cycle regulation has emerged. Genetic alteration of glucuronide met...

journal_title：Biotechnology letters

authors： Woo HH,Jeong BR,Hawes MC

更新日期：2005-03-01 00:00:00

abstract：:Peroxisome proliferator-activated receptors (PPARs) alpha and gamma are key regulators of lipid homeostasis and insulin resistance. In this study we show that a novel compound, 3-{4-[2-(5-methyl-2-phenyl-oxazol-4-yl)-ethoxy]-phenyl}- 2-[2-(2-nitro-phenoxy)-acetyl amino]-propionic acid (O325H), is an agonist with dual ...

journal_title：Biotechnology letters

authors： Xu C,Wang LL,Liu HY,Ruan CM,Zhou XB,Cao YL,Li S

更新日期：2006-06-01 00:00:00

abstract：OBJECTIVE:The growth characteristics and intracellular hemin concentrations of Lactococcus lactis grown under different culture pH and aeration conditions were examined to investigate the effect of culture pH on the respiration efficiency of L. lactis NZ9000 (pZN8148). RESULTS:Cell biomass and biomass yield of L. lact...

journal_title：Biotechnology letters

authors： Shi W,Li Y,Gao X,Fu R

更新日期：2016-03-01 00:00:00

abstract：OBJECTIVES:Interactions of cells with their neighbors and influences by the surrounding extracellular matrix (ECM) is reflected in a cells transcriptome and proteome. In tissues comprised of heterogeneous cell populations or cells depending on ECM signalling cues such as those of the intervertebral disc (IVD), this inf...

journal_title：Biotechnology letters

authors： Li K,Varden L,Henderson A,Lufkin T,Kraus P

更新日期：2021-01-01 00:00:00

abstract：:The combined analysis of peptide mass fingerprinting and 2-DE/MS using the induced and selected protein spots following growth of Pseudomonas sp. DU102 on benzoate or p-hydroxybenzoate revealed not only alpha- and beta-subunits of protocatechuate 3,4-dioxygenase but also catechol 1,2-dioxygenase responsible for ortho-...

journal_title：Biotechnology letters

authors： Kim SI,Park SH,Kim JW,Leem SH,Shin DJ,Kim SH,Lee DH,Kahng HY

更新日期：2007-10-01 00:00:00

abstract：:A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation without significant loss of transformation efficiency i...

journal_title：Biotechnology letters

authors： Ravnikar M,Irman A,Radić N,Lunder M,Strukelj B

更新日期：2009-12-01 00:00:00

abstract：:A strain of Pseudomonas stutzeri ZWLR2-1 was isolated from soil contaminated with chloronitrobenzenes and identified by 16S rDNA sequencing. This bacterium released chloride and nitrite into the medium when grown on 0.5 mM 2-chloronitrobenzene. PCR amplification and DNA sequencing revealed a DNA fragment encoding a po...

journal_title：Biotechnology letters

authors： Liu H,Wang SJ,Zhou NY

更新日期：2005-02-01 00:00:00

abstract：:The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop. To distinguish male genotypes from female genotypes early in the vegetative growth phase, we have developed robust PCR-based marker(s). DNA bulk samples from 20 male and 20 female plants each were screened ...

journal_title：Biotechnology letters

authors： Korekar G,Sharma RK,Kumar R,Meenu,Bisht NC,Srivastava RB,Ahuja PS,Stobdan T

更新日期：2012-05-01 00:00:00

abstract：OBJECTIVES:We examined the importance of aptamer usage under the same condition as the selection process by employing the previously selected aptamers for calmodulin (CaM) which includes a non-natural fluorogenic amino acid, 7-nitro-2,1,3-benzoxadiazole. RESULTS:We added five amino acids at the N-terminus which was em...

journal_title：Biotechnology letters

authors： Manandhar Y,Wang W,Inoue J,Hayashi N,Uzawa T,Ito Y,Aigaki T,Ito Y

更新日期：2017-03-01 00:00:00

abstract：:Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 de...

journal_title：Biotechnology letters

authors： Glendinning KJ,Macaskie LE,Brown NL

更新日期：2005-11-01 00:00:00

abstract：:Pleurotus eryngii was transformed using a polyethylene glycol-mediated method. A plasmid, pEPUGH, containing a reporter gene (enhanced green fluorescent protein gene, egfp) and a positive selectable marker gene (hygromycin phosphotransferase gene, hph) was constructed. The fused egfp-hph gene was placed under the cont...

journal_title：Biotechnology letters

authors： Yin Y,Liu Y,Jin H,Wang S,Zhao S,Geng X,Li M,Xu F

更新日期：2012-10-01 00:00:00

abstract：:A number of biosensors have been developed for phosphate analysis particularly, concerning its negative impact within the environmental and biological systems. Enzymatic biosensors comprising either a single or multiple enzymatic system have been extensively used for the direct and indirect analysis of phosphate ions....

journal_title：Biotechnology letters

authors： Upadhyay LS,Verma N

更新日期：2015-07-01 00:00:00

abstract：:Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...

journal_title：Biotechnology letters

authors： Jin X,Rao M,Wei W,Ge M,Liu J,Chen D,Liang Y

更新日期：2012-12-01 00:00:00

abstract：:The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene su...

journal_title：Biotechnology letters

authors： Lim HA,Raku T,Tokiwa Y

更新日期：2005-04-01 00:00:00

abstract：:Decrystallized chitosan was produced from shrimp shells with a low degree of crystallinity (10%) and a high anionic dye binding capacity. Raw, mixed dye wastewater from a textile factory was efficiently decolorized using decrystallized chitosan that was more efficient than using normal chitosan and activated carbon. D...

journal_title：Biotechnology letters

authors： Trung TS,Ng CH,Stevens WF

更新日期：2003-07-01 00:00:00

abstract：:Cell-based therapeutics are promising routes for the regeneration of damaged cells and organs. The recovery of cells cultured in vitro for such applications requires the use of proteolytic enzymes which deteriorate its property by disruption of cell-cell and cell-matrix interactions. Intact cell sheets can be retrieve...

journal_title：Biotechnology letters

authors： Mukundan LM,Nirmal R,Thomas LV,Sajeev US,Nair PD

更新日期：2011-10-01 00:00:00

abstract：:The gene, xynB, from Streptomyces olivaceoviridis A1 encoding xylanase, XYNB, with a high specific activity for xylan, was transformed into potato (Solanum tuberosum L.) by Agrobacterium tumefaciens. The integration of xynB into genomic DNA was confirmed by PCR and reverse transcriptase-PCR. The gene was expressed und...

journal_title：Biotechnology letters

authors： Yang P,Wang Y,Bai Y,Meng K,Luo H,Yuan T,Fan Y,Yao B

更新日期：2007-04-01 00:00:00

abstract：:Indole-3-butyric acid at 25 microM with methyl jasmonate (MJ) at 100 microM in Panax ginseng synergistically stimulated both root growth and ginsenoside accumulation compared with 100 microM MJ alone. Productivity of ginsenoside was 10 mg l(-1) d(-1) compared to 7.3 mg l(-1) d(-1) with MJ elicitation alone. ...

journal_title：Biotechnology letters

authors： Kim YS,Yeung EC,Hahn EJ,Paek KY

更新日期：2007-11-01 00:00:00

abstract：OBJECTIVES:To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights. RESULTS:Cellulosimicrobium (family Promicromonosporaceae) ...

journal_title：Biotechnology letters

authors： Wang W,Yu Y,Dou TY,Wang JY,Sun C

更新日期：2018-02-01 00:00:00

abstract：:Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytube...

journal_title：Biotechnology letters

authors： Komaraiah P,Reddy GV,Reddy PS,Raghavendra AS,Ramakrishna SV,Reddanna P

更新日期：2003-04-01 00:00:00