Abstract:
:A new method for fast transformation of competent bacterial cells has been developed. The transformation is induced with cholic acid analogues or saponins which cause reversible disruption of the bacterial membrane. This method shortens the time of transformation without significant loss of transformation efficiency in comparison to heat shock method and is the first reported chemically-induced transformation. New data about interactions between cholates and biomembranes is revealed that may contribute to better understanding of bacterial transformation.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Ravnikar M,Irman A,Radić N,Lunder M,Strukelj Bdoi
10.1007/s10529-009-0101-7subject
Has Abstractpub_date
2009-12-01 00:00:00pages
1943-6issue
12eissn
0141-5492issn
1573-6776journal_volume
31pub_type
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