Abstract:
:Quercetin was methylated with two O-methyltransferases (OMTs) expressed in E. coli. A construct (RSOMT) was designed to express two OMTs: ROMT-9, which methylates specifically at the 3'-hydroxyl group of quercetin and SOMT-2, which methylates at the 4'-hydroxyl group. Both OMT genes were driven by T7 promoters and had ribosome binding sites. Both ROMT-9 and SOMT-2 were successfully expressed in E. coli transformant harboring RSOMT. Reaction products of quercetin with E. coli transformant containing RSOMT showed two methylation products that corresponded to the 3'-methylated and the 3',4'-dimethylated quercetin, which were confirmed by NMR. More than 90% of quercetin was converted into the 3',4'-dimethylated quercetin after 24 h incubation with E. coli transformant harboring RSOMT.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Kim BG,Shin KH,Lee Y,Hur HG,Lim Y,Ahn JHdoi
10.1007/s10529-005-3893-0keywords:
subject
Has Abstractpub_date
2005-12-01 00:00:00pages
1861-4issue
23-24eissn
0141-5492issn
1573-6776journal_volume
27pub_type
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