Abstract:
:A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of six organic solvents with logP values approximately between 1 and -1. This assay delivered results within comparatively short times measured by a color reaction and thus facilitates the choice of an enzyme-solvent combination for the synthesis of glycolipids. To validate the findings, glycolipid syntheses were performed using the same lipase formulation in the same solvents. When comparing the results obtained using the microtiter plate-based assay to the results of the glycolipid syntheses using the same lipases and solvents, matching results were obtained.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Pöhnlein M,Finkbeiner T,Syldatk C,Hausmann Rdoi
10.1007/s10529-014-1721-0subject
Has Abstractpub_date
2015-03-01 00:00:00pages
705-10issue
3eissn
0141-5492issn
1573-6776journal_volume
37pub_type
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