Re-engineering Escherichia coli for ethanol production.

abstract：:Two new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene (msgA) that converts dihydroxyacetone-phosphate to methylglyoxal, a precursor for both L: (+)- and D: (-)-lactate. Strain TG113 con...

journal_title：Biotechnology letters

authors： Grabar TB,Zhou S,Shanmugam KT,Yomano LP,Ingram LO

更新日期：2006-10-01 00:00:00

abstract：:Serratia marcescens SS-1 and its SpnR-defective isogenic mutant, SMdeltaR, produced an extracellular surfactant able to decrease surface tension of water from 72 to 37 dyne cm(-1) (SMdeltaR strain) and to 45 dyne cm(-1) (SS-1 strain). The biosurfactant also emulsified kerosene and diesel with a maximum emulsion index ...

journal_title：Biotechnology letters

authors： Wei YH,Lai HC,Chen SY,Yeh MS,Chang JS

更新日期：2004-05-01 00:00:00

abstract：:A hybrid baculovirus, a hybrid of the Autographa californica nuclear polyhedrosis virus and the Bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of bovine interleukin-21 (IL-21) in silkworms. A recombinant hybrid baculovirus containing the full length of the cDNA of bovine interleukin-21...

journal_title：Biotechnology letters

authors： Muneta Y,Nagaya H,Minagawa Y,Enomoto C,Matsumoto S,Mori Y

更新日期：2004-09-01 00:00:00

abstract：:The recombinant laccase from Thermus thermophilus was applied to the biobleaching of wheat straw pulp. The best bleaching effect was when the pulp was treated with 3 U laccase g(-1) dry pulp at 90°C, pH 4.5, 8% consistency for 1.5 h. Under these conditions, the pulp brightness was increased by 3.3% ISO, and the pulp k...

journal_title：Biotechnology letters

authors： Zheng Z,Li H,Li L,Shao W

更新日期：2012-03-01 00:00:00

abstract：:Deacetoxy/deacetylcephalosporin C synthase (acDAOC/DACS) from Acremonium chrysogenum is a bifunctional enzyme that catalyzes both the ring-expansion of penicillin N to deacetoxycephalosporin C and the hydroxylation of the latter to deacetylcephalosporin C. The R308 residue located in close proximity to the C-terminus ...

journal_title：Biotechnology letters

authors： Wu XB,Tian XY,Ji JJ,Wu WB,Fan KQ,Yang KQ

更新日期：2011-04-01 00:00:00

abstract：OBJECTIVES:To rationally identify targets for enhancing adenosine production, transcription level of genes involved in adenosine synthesis of Bacillus subtilis XGL was detected during the fermentation process, complemented with metabolite pool analysis. RESULTS:PurR-regulated genes (pur operon and purA) and prs were d...

journal_title：Biotechnology letters

authors： Zhang C,Du S,Liu Y,Xie X,Xu Q,Chen N

更新日期：2015-07-01 00:00:00

abstract：:Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hy...

journal_title：Biotechnology letters

authors： Wu J,Zhang HB,Wang J,Yang TC,Xian J,Yang CH,Lai HW

更新日期：2008-06-01 00:00:00

abstract：:Fourteen yeast strains were screened for production of 2-phenylethanol from L-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l-1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-pheny...

journal_title：Biotechnology letters

authors： Etschmann MM,Sell D,Schrader J

更新日期：2003-04-01 00:00:00

abstract：:The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix o...

journal_title：Biotechnology letters

authors： Camperi SA,Iannucci NB,Albanesi GJ,Oggero Eberhardt M,Etcheverrigaray M,Messeguer A,Albericio F,Cascone O

更新日期：2003-09-01 00:00:00

abstract：OBJECTIVES:To investigate the effect of full-length fragment of DNA topoisomerase I gene (TOP1) matrix attachment regions (MARs) originating from the human genome on transgene expression in Chinese hamster ovary (CHO) cells and explore the underlying mechanisms. RESULTS:Results showed that TOP1 MAR cannot only enhance...

journal_title：Biotechnology letters

authors： Jia YL,Guo X,Wang XC,Wang TY

更新日期：2019-07-01 00:00:00

abstract：OBJECTIVES:To identify and characterize a novel antimicrobial peptide, catesbeianin-1. RESULTS:Catesbeianin-1 is 25 amino acids long and is α-helical, cationic and amphipathic. It had antimicrobial activity against Gram-positive and Gram-negative bacteria. It was resistant against trypsin and pepsin. Catesbeianin-1 ex...

journal_title：Biotechnology letters

authors： Xu H,Zhang Y,Feng X,Tie K,Cao Y,Han W

更新日期：2017-06-01 00:00:00

abstract：:A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly conserved among serine...

journal_title：Biotechnology letters

authors： Xie Z,Xu B,Ding J,Liu L,Zhang X,Li J,Huang Z

更新日期：2013-08-01 00:00:00

abstract：:The promoter region of the pur operon, which contains 12 genes for inosine monophosphate biosynthesis from phosphoribosylpyrophosphate, and the purA gene, encoding the adenylosuccinate synthetase, were compared among wild-type and three purine-producing Bacillus subtilis strains. A single nucleotide deletion at positi...

journal_title：Biotechnology letters

authors： Qian J,Cai X,Chu J,Zhuang Y,Zhang S

更新日期：2006-06-01 00:00:00

abstract：:Different strains of Micrococcus luteus, isolated from high-altitude Argentinean wetlands, were recently reported to harbour the linear plasmids pLMA1, pLMH5 and pLMV7, all of which with 5'-covalently attached terminal proteins. The link between pLMA1 and the host's erythromycin resistance as well as further presumpti...

journal_title：Biotechnology letters

authors： Wagenknecht M,Dib JR,Thürmer A,Daniel R,Farías ME,Meinhardt F

更新日期：2010-12-01 00:00:00

abstract：:To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) ...

journal_title：Biotechnology letters

authors： Zhang Q,Lee HG,Kang SK,Baik M,Choi YJ

更新日期：2014-06-01 00:00:00

abstract：:A novel method to examine the morphology and structure of fungal hyphae in solid pastes used for the production of meat alternative product is presented. A sample of fermentation broth was fluorescently stained with Calcofluor White and added back to the broth, mixed and then a paste made using ultra-filtration. Fibre...

journal_title：Biotechnology letters

authors： Miri T,Cox PW,Fryer PJ

更新日期：2003-02-01 00:00:00

abstract：:In this study, 3D culture system for human adipose-derived stem cell (hASC) using a BioLevitator as the bioreactor for microcarrier-based cultures was established. During the culturing period, hASCs preferred to grow in crevices between microcarriers and a high viability was maintained even when reaching confluency. A...

journal_title：Biotechnology letters

authors： Lin CY,Huang CH,Wu YK,Cheng NC,Yu J

更新日期：2014-07-01 00:00:00

abstract：:Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored dir...

journal_title：Biotechnology letters

authors： van Eijsden RG,Stassen C,Daenen L,Van Mulders SE,Bapat PM,Siewers V,Goossens KV,Nielsen J,Delvaux FR,Van Hummelen P,Devreese B,Willaert RG

更新日期：2013-06-01 00:00:00

abstract：:Laccases play an important role in the biological break down of lignin and have great potential in the deconstruction of lignocellulosic feedstocks. We examined 16 laccases, both commercially prepared and crude extracts, for their ability to oxidize veratryl alcohol in the presence of various solvents and mediators. S...

journal_title：Biotechnology letters

authors： Larson TM,Anderson AM,Rich JO

更新日期：2013-02-01 00:00:00

abstract：:General methods for identification of usual carotenoids including C18-HPLC retention time and spectroscopic methods, such as absorption spectra, mass spectra, NMR spectra, are briefly summarized. ...

journal_title：Biotechnology letters

authors： Takaichi S

更新日期：2014-06-01 00:00:00

abstract：:Ma-pyrG was cloned from Monascus aurantiacus AS3.4384 using degenerate PCR with primers designed with an algorithm called CODEHOP, and its complete sequence was obtained by a PCR-based strategy for screening a Monascus fosmid library. Ma-pyrG encodes orotidine-5'-phosphate decarboxylase (OMPdecase), a 283-aminoacid pr...

journal_title：Biotechnology letters

authors： Wang BH,Xu Y,Li YP

更新日期：2010-11-01 00:00:00

abstract：:The effects of the sequential application of methyl jasmonate (MeJa), salicylic acid (SA) and yeast extract (YE) to Argemone mexicana cell cultures were compared to either the sole application of each elicitor, or to the three-partite mixture. The highest sanguinarine accumulation occurred using the sequential treatme...

journal_title：Biotechnology letters

authors： Trujillo-Villanueva K,Rubio-Piña J,Monforte-González M,Ramírez-Benítez E,Vázquez-Flota F

更新日期：2012-02-01 00:00:00

abstract：:Gluconobacter oxydans LMG 1489 was selected as the best strain for NAD(P)-dependent polyol dehydrogenase production. The highest enzyme activities were obtained when this strain was cultivated on a medium consisting of 30 g glycerol l(-1), 7.2 g peptone l(-1) and 1.8 g yeast extract l(-1). Two D-fructose reducing, NAD...

journal_title：Biotechnology letters

authors： Parmentier S,Beauprez J,Arnaut F,Soetaert W,Vandamme EJ

更新日期：2005-03-01 00:00:00

abstract：OBJECTIVE:To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity. RESULTS:A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most ...

journal_title：Biotechnology letters

authors： Zhang D,Jia H,Wang Y,Li WM,Hou YC,Yin SW,Wang TD,He SX,Lu SY

更新日期：2015-11-01 00:00:00

abstract：:Long-term cell recycle fermentations of Candida tropicalis were performed over 14 rounds of fermentation. The average xylitol concentrations, fermentation times, volumetric productivities and product yields for 14 rounds were 105 g l(-1), 333 h, 4.4 g l(-1) h(-1) and 78%, respectively, in complex medium; and 110 g l(-...

journal_title：Biotechnology letters

authors： Kim TB,Lee YJ,Kim P,Kim CS,Oh DK

更新日期：2004-04-01 00:00:00

abstract：:In batch culture for glutathione production with Candida utilis, a higher temperature (30 degrees C) was required to hasten cell growth while a lower temperature (26 degrees C) was needed to increase the production of glutathione. A two-stage temperature control strategy was used to enhance both the yield and the prod...

journal_title：Biotechnology letters

authors： Wei G,Li Y,Du G,Chen J

更新日期：2003-06-01 00:00:00

abstract：:Anthocyanic vacuolar inclusions (AVIs) appear as dark red-to-purple spheres of various sizes in vacuoles of grapevine (Vitis vinifera L.) cell suspension culture due to their interaction with anthocyanins. AVIs were purified and the bound anthocyanins extracted and analysed by HPLC from two lines of V. vinifera isolat...

journal_title：Biotechnology letters

authors： Conn S,Zhang W,Franco C

更新日期：2003-06-01 00:00:00

abstract：:A single-compartmented microbial fuel cell composed of a graphite felt anode modified with Neutral Red (NR-anode) and a porous Fe(II)-carbon cathode (FeC-cathode) were compared for electricity generation from Microbacterium sp. and Pseudomonas sp. under identical conditions. Pseudomonas sp. was more than four times th...

journal_title：Biotechnology letters

authors： Lee SW,Jeon BY,Park DH

更新日期：2010-04-01 00:00:00

abstract：:A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...

journal_title：Biotechnology letters

authors： Tounsi S,Jaoua S

更新日期：2003-08-01 00:00:00

abstract：OBJECTIVE:To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. RESULTS:Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate,...

journal_title：Biotechnology letters

authors： Shariati L,Modaress M,Khanahmad H,Hejazi Z,Tabatabaiefar MA,Salehi M,Modarressi MH

更新日期：2016-08-01 00:00:00