Abstract:
OBJECTIVE:To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity. RESULTS:A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most highly selective peptide, which termed RP-1. Immunofluorescence and flow cytometry analysis indicated that the consensus peptide RP-1 could bind to CD44-positive gastric cancer cells with mean fluorescence intensities significantly higher than that of CD44-negative cells. CD44 knockdown led to decreased binding activity of RP-1 to the same cell line. Tissue array technique was used to identify the relationship (r = 0.556) between peptide binding and CD44 detection on gastric cancer tissues. Further, the hyaluronan-binding domain of CD44 was docked with RP-1 using computer modeling/docking approaches, revealing a RP-1/CD44 interaction with geometrical and energy match (-8.6 kcal/mol). CONCLUSIONS:The RP-1 peptide we screened exhibits affinity and specificity to CD44 on cells and has the potential to be used as a candidate probe for gastric cancer cell targeting.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Zhang D,Jia H,Wang Y,Li WM,Hou YC,Yin SW,Wang TD,He SX,Lu SYdoi
10.1007/s10529-015-1896-zsubject
Has Abstractpub_date
2015-11-01 00:00:00pages
2311-20issue
11eissn
0141-5492issn
1573-6776pii
10.1007/s10529-015-1896-zjournal_volume
37pub_type
杂志文章abstract::The conventional method of transketolase (TKT) activity assay uses ribose 5-phosphate and xylulose 5-phosphate as substrates. However, a new method of TKT assay is currently required since xylulose 5-phosphate is no longer commercially available and is difficult to synthesize chemically. Although there are effective a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9616-y
更新日期:2008-05-01 00:00:00
abstract:OBJECTIVES:Breast cancer is a popular fatal malignant tumor for women with high of rates incidence and mortality. Development of the new approaches for breast cancer targeted diagnosis and chemotherapy is emergently needed by the current clinical practice, the important first step is finding a breast cancer specificall...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03044-3
更新日期:2021-01-01 00:00:00
abstract::The aim of this study was to characterize the growth of the fungus Leucoagaricus gongylophorus LEU18496, isolated from the fungus garden of the nest of leaf cutter ants Atta mexicana. The fungus garden was cultivated in an artificial laboratory nest and the fungus further grown in submerged (SmC) and solid state (SSC)...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03057-y
更新日期:2021-01-02 00:00:00
abstract::Several alkyl-substituted methoxysilanes were evaluated as potential activity and stability enhancing agents for biomimetic silicification of Rhodosporidium toruloides D-amino acid oxidase (RtDAO). When methyl-substituted silanes along with tetramethoxysilane were used as silicic acid precursors for polyallylamine (PA...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0924-5
更新日期:2012-08-01 00:00:00
abstract:OBJECTIVE:To develop a mouse artificial chromosome (MAC) carrying the mouse Xist gene (X-inactive specific transcript; Xist-MAC) as a systematic in vitro approach for investigating Xist RNA-mediated chromosome inactivation. RESULTS:Ectopic expression of the Xist gene in CHO cells led to the accumulation of Xist RNA in...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02826-z
更新日期:2020-05-01 00:00:00
abstract::N(omega)-2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl (N(omega)-Pbf)-protected oligoarginine was directly conjugated to poly(amino acid) derivatives modified with a long alkyl chain. The final concentration of conjugated peptides was easily controlled by the feed ratio of oligoarginine to polymer backbone and a f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-7838-4
更新日期:2005-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1801-9
更新日期:2015-07-01 00:00:00
abstract::The extracellular domains (ECD) of epidermal growth factor receptors, ErbB1, 2, 3 and 4, were designed as soluble dimeric forms. Each ECD was fused to a short hinge region derived from IgG, such that the stable dimer could be formed with disulfide bridges. This hinge-tagged design minimized the molecular weight to app...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0160-9
更新日期:2010-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0238-4
更新日期:2010-07-01 00:00:00
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更新日期:2014-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2394-2
更新日期:2017-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2723-8
更新日期:2005-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2424-0
更新日期:2017-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1971-5
更新日期:2016-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9643-3
更新日期:2008-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-4682-5
更新日期:2006-01-01 00:00:00
abstract::An endoxylanase gene, xynA, was cloned from Bacillus pumilus ARA and expressed in Escherichia coli. The open reading frame of the xynA gene was 687 bp encoding a signal peptide and a mature xylanase with a molecular mass of 23 kDa. The enzyme was categorized as a glycosyl hydrolase family 11 member based on the sequen...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0568-x
更新日期:2011-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000025890.97067.98
更新日期:2004-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0088-0
更新日期:2009-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9717-2
更新日期:2008-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2212-2
更新日期:2017-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-0938-3
更新日期:2005-09-01 00:00:00
abstract:OBJECTIVES:To develop a rapid, dual-parameter, plate-based screening process to improve production and secretion rate of glucose oxidase simultaneously in Aspergillus niger. RESULTS:A morphology engineering based on CaCO3 was implemented, where the yield of GOD by A. niger was increased by up to 50%. Analysis of extra...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2442-y
更新日期:2018-01-01 00:00:00
abstract::A purified recombinant enzyme from Spirochaeta thermophila, that is suggested to be a cellobiose 2-epimerase, was a 47 kDa monomer with a specific activity of 29.2 U min(-1) for mannobiose. The epimerization activity of the recombinant enzyme for mannobiose was maximal at pH 7.0 and 60 °C with a half-life of 124 h. Th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1267-6
更新日期:2013-11-01 00:00:00
abstract::The genes involved in the biosynthetic pathway of ectoine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid) from Bacillus halodurans were cloned as an operon and expressed in E. coli. Analysis of the deduced ectoine biosynthesis cluster amino acid sequence revealed that the ectoine operon contain 2,389 bp, enc...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9688-3
更新日期:2008-08-01 00:00:00
abstract::Pectinase was immobilized on a sodium alginate support using glutaraldehyde and retained 66% activity. The optimal pH for activity shifted from 3.0 to 3.5 after immobilization; however, the optimum temperature remained unchanged at 40 degrees C. The immobilized enzyme also had a higher thermal stability and reusabilit...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9409-3
更新日期:2007-09-01 00:00:00
abstract::5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0943-2
更新日期:2012-09-01 00:00:00
abstract::Of several types of chemical surfactants and biosurfactants, only the culture broth of Bacillus subtilis C1 containing surfactin at 10 mg l(-1) completely inhibited the growth of Microcystis aeruginosa, a bloom-forming cyanobacterium in highly eutrophic lakes. The broth with 10 mg surfactin l(-1) also removed 85% of t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024508927361
更新日期:2003-07-01 00:00:00
abstract:OBJECTIVE:To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations RESULTS:Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2087-2
更新日期:2016-07-01 00:00:00
abstract::Aerobic granules for sulphide and ammonium removal were cultivated in a sequencing batch reactor, and the microbial community of the aerobic granules was investigated by denaturing gradient gel electrophoresis. The loading rate increased from 0.15 to 0.9 kg S2− m−3 d−1, and the removal efficiencies of sulphide, chemic...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0857-z
更新日期:2012-05-01 00:00:00