New aqueous two phase system comprising polyethylene glycol and xanthan.

abstract：:This review concerns basic research on cellulases and cellulose-specific carbohydrate-binding modules (CBMs). As a background, glycosyl hydrolases are also briefly reviewed. The nomenclature of cellulases and CBMs is discussed. The main cellulase-producing organisms and their cellulases are described. Synergy, enantio...

journal_title：Biotechnology letters

authors： Hildén L,Johansson G

更新日期：2004-11-01 00:00:00

abstract：:A plasmid, pBGFP, carrying green fluorescent protein (gfp) and benomyl-resistance genes was constructed and transformed into Metarhizium anisopliae. The transformants grew normally and GFP fluorescence was detected. No change was found in virulence for the transformants. Fluorescence was detected in hyphae from the ha...

journal_title：Biotechnology letters

authors： Cao Y,Peng G,He Z,Wang Z,Yin Y,Xia Y

更新日期：2007-06-01 00:00:00

abstract：OBJECTIVE:To make the previously developed biosynthesis of L-2-aminobutyric acid (L-ABA) more suitable for the industrial-scale production. RESULTS:A recyclable biotransformation system was developed based on immobilized enzyme technology. The conversion yield of L-threonine (at 90 g l(-1)) reached 99.9 % and the theo...

journal_title：Biotechnology letters

authors： Xu G,Jiang Y,Tao R,Wang S,Zeng H,Yang S

更新日期：2016-01-01 00:00:00

abstract：:Peptides are emerging as pharmaceutical agents in cancer therapy. The peptide, TLSGAFELSRDK (TLS) is a targeting ligand that can specifically triggers cellular uptake by binding to SKOV3 cells. Cell surface proteins and the C-terminal basic residues of the TLS are required for effective cell penetration, and the uptak...

journal_title：Biotechnology letters

authors： Ma C,Yin G,You F,Wei Y,Huang Z,Chen X,Yan D

更新日期：2013-11-01 00:00:00

abstract：:The gene, xynB, from Streptomyces olivaceoviridis A1 encoding xylanase, XYNB, with a high specific activity for xylan, was transformed into potato (Solanum tuberosum L.) by Agrobacterium tumefaciens. The integration of xynB into genomic DNA was confirmed by PCR and reverse transcriptase-PCR. The gene was expressed und...

journal_title：Biotechnology letters

authors： Yang P,Wang Y,Bai Y,Meng K,Luo H,Yuan T,Fan Y,Yao B

更新日期：2007-04-01 00:00:00

abstract：OBJECTIVE:To investigate the aerotolerance of Lactobacillus rhamnosus hsryfm 1301 and its influencing factors. RESULTS:The growth rate of L. rhamnosus hsryfm 1301 weakened noticeably when the concentration of supplemented H2O2 reached 1 mM, and only 2% of all L. rhamnosus hsryfm 1301 cells survived in MRS broth supple...

journal_title：Biotechnology letters

authors： Zhang C,Lu J,Yang D,Chen X,Huang Y,Gu R

更新日期：2018-04-01 00:00:00

abstract：:miR-21 is highly expressed in a variety of cancers, suggesting that it might play a role in the process of oncogenesis, as supported by it directly causing pre-B cell lymphomas in transgenic mice. Rm21LG transgenic mice for the conditional co-expression of miR-21 and luciferase (Luc) mediated by Cre/lox P system were ...

journal_title：Biotechnology letters

authors： Du T,Jia J,Lin X,Xie R,Li J,Xiao D,Xu K

更新日期：2014-01-01 00:00:00

abstract：:Mucor circinelloides and Mortierella isabellina hydroxylated dehydroabietic acid (DehA). DehA was converted regio- and stereo-selectively by whole cells of Mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mM from 14.7 mM DehA) after 72 h in the cultivation medium contain...

journal_title：Biotechnology letters

authors： Mitsukura K,Imoto T,Nagaoka H,Yoshida T,Nagasawa T

更新日期：2005-09-01 00:00:00

abstract：:Genes coding for Vitreoscilla hemoglobin (VHb) with peroxisome targeting signal (PTS1) tag and beta-galactosidase were co-expressed in Pichia pastoris under the alcohol oxidase1 (AOX1) promoter. The expression of VHb-PTS1 had no positive effect on cell growth but significantly enhanced the whole cell beta-galactosidas...

journal_title：Biotechnology letters

authors： Wu JM,Hsu TA,Lee CK

更新日期：2003-09-01 00:00:00

abstract：:Microbial fuel cells can be designed to remove nitrogenous compounds out of wastewater, but their performance is at present limited to 0.33 kg NO(3) (-)-Nm(-3) net cathode compartment (NCC) d(-1). By maintaining the pH in the cathode at 7.2, nitrogen removal was increased from 0.22 to 0.50 kg NO(3) (-)-Nm(-3) NCC d(-1...

journal_title：Biotechnology letters

authors： Clauwaert P,Desloover J,Shea C,Nerenberg R,Boon N,Verstraete W

更新日期：2009-10-01 00:00:00

abstract：:The recombinant laccase from Thermus thermophilus was applied to the biobleaching of wheat straw pulp. The best bleaching effect was when the pulp was treated with 3 U laccase g(-1) dry pulp at 90°C, pH 4.5, 8% consistency for 1.5 h. Under these conditions, the pulp brightness was increased by 3.3% ISO, and the pulp k...

journal_title：Biotechnology letters

authors： Zheng Z,Li H,Li L,Shao W

更新日期：2012-03-01 00:00:00

abstract：:Microcycle conidiation is a survival mechanism of fungi encountering unfavorable conditions. In this phenomenon, asexual spores germinate secondary spores directly without formation of mycelium. As Penicillium camemberti conidia have the ability to produce conidiophores after germination in liquid culture induced by a...

journal_title：Biotechnology letters

authors： Boualem K,Gervais P,Cavin JF,Waché Y

更新日期：2014-11-01 00:00:00

abstract：:Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degree C was nearly 35% of th...

journal_title：Biotechnology letters

authors： Zheng Z,Jiang YH,Miao JL,Wang QF,Zhang BT,Li GY

更新日期：2006-01-01 00:00:00

abstract：:The shuttle vector pGYC4α (6,157 bp) was constructed based on the sigma-replicon plasmid pYC2 from Lactobacillus sakei BM5 isolated from kimchi. The vector contained inserts of the ColE1 replicon, α-amylase gene from Bacillus licheniformis containing its own signal peptide, and lactococcal promoter P32. Transformation...

journal_title：Biotechnology letters

authors： Yang EJ,Chang HC

更新日期：2011-03-01 00:00:00

abstract：:Biotransformation of 1,3-dichloro-2-propanol (DCP) to epichlorohydrin (ECH) by the whole cells of recombinant Escherichia coli expressing halohydrin dehalogenase was limited by product inhibition. To solve this problem and improve the ECH yield, a biotransformation strategy using resin-based in situ product removal (I...

journal_title：Biotechnology letters

authors： Zou SP,Du EH,Hu ZC,Zheng YG

更新日期：2013-06-01 00:00:00

abstract：OBJECTIVES:To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system. RESULTS:An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM c...

journal_title：Biotechnology letters

authors： Jin L,Wang ZS,Cao Y,Sun RQ,Zhou H,Cao RY

更新日期：2021-02-01 00:00:00

abstract：OBJECTIVES:The aim of the study is to generate a spherical three-dimensional (3D) aggregate of hepatocyte-like cells (HLCs) differentiated from human embryonic stem cells and to investigate the effect of the 3D environment on hepatic maturation and drug metabolism. RESULTS:Quantitative real-time PCR analysis indicated...

journal_title：Biotechnology letters

authors： Choi YJ,Kim H,Kim JW,Yoon S,Park HJ

更新日期：2018-05-01 00:00:00

abstract：:Human intestinal maltase (HMA) is an α-glucosidase that hydrolyses α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA is an important target to discover of new drugs for the treatment of type 2 diabetes. In this study, 308,307 compounds were virtually screened with HMA using Autodock 3.0.5 in a WI...

journal_title：Biotechnology letters

authors： Nguyen TT,Ryu HJ,Lee SH,Hwang S,Cha J,Breton V,Kim D

更新日期：2011-11-01 00:00:00

abstract：OBJECTIVE:To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations RESULTS:Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl...

journal_title：Biotechnology letters

authors： Liu C,Li LJ,Wu CY,Guo KN,Li JH

更新日期：2016-07-01 00:00:00

abstract：:Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O(2) transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g ...

journal_title：Biotechnology letters

authors： Díaz-Barrera A,Silva P,Avalos R,Acevedo F

更新日期：2009-06-01 00:00:00

abstract：:A 4 kb BamHI-HindIlI fragment, corresponding to the cry2A operon of Bacillus thuringiensis subsp. kurstaki strain BNS3, was cloned. The sequencing of the corresponding cry2Aa-type gene, termed crybns3-4, revealed an open reading frame of 1902 bp, encoding a protein of 633 amino-acid residues. Both nucleotide and amino...

journal_title：Biotechnology letters

authors： Tounsi S,Jaoua S

更新日期：2003-08-01 00:00:00

abstract：OBJECTIVES:Catalytic efficiency of a nitrogen-doped, mesoporous carbon aerogel cathode catalyst was investigated in a two-chambered microbial fuel cell (MFC) applying graphite felt as base material for cathode and anode, utilizing peptone as carbon source. RESULTS:This mesoporous carbon aerogel containing catalyst lay...

journal_title：Biotechnology letters

authors： Tardy GM,Lóránt B,Lóka M,Nagy B,László K

更新日期：2017-07-01 00:00:00

abstract：:The limiting step for biosynthesis of 3-hydroxypropionic acid (3-HP) in Klebsiella pneumoniae is the conversion of 3-hydroxypropionaldehyde (3-HPA) to 3-HP. This reaction is catalyzed by aldehyde dehydrogenase (ALDH) with NAD(+) as a cofactor. Although NAD(+)-dependent ALDH overexpression facilitates 3-HP biosynthesis...

journal_title：Biotechnology letters

authors： Li Y,Liu L,Tian P

更新日期：2014-11-01 00:00:00

abstract：:Cellobiose is a major intermediate from cellulase hydrolysis of pretreated plant biomass. Engineering biocatalysts for direct use of cellobiose could eliminate the need for exogenous β-glucosidase. Additionally, rapid removal of cellobiose in a simultaneous saccharification and fermentation facilitates enzymatic hydro...

journal_title：Biotechnology letters

authors： Rutter C,Chen R

更新日期：2014-02-01 00:00:00

abstract：OBJECTIVE:Silk sericin is a natural polymer with potential utility in biomedical and biotechnological applications. Recombinantly expressed sericin ensures a source of pure protein with no contamination and with multiple properties when expressed as a fusion protein. Hence, the present paper aims to recombinantly expre...

journal_title：Biotechnology letters

authors： Thomas DS,Manoharan C,Rasalkar S,Mishra RK,Gopalapillai R

更新日期：2020-09-01 00:00:00

abstract：OBJECTIVES:The purpose of this study was to develop a facile and efficient method to enhance the stability and activity of lactoperoxidase (LPO) by using its immobilization on graphene oxide nanosheets (GO-NS). METHODS:Following the LPO purification from bovine whey, it was immobilized onto functionalized GO-NS using ...

journal_title：Biotechnology letters

authors： Shariat SZAS,Borzouee F,Mofid MR,Varshosaz J

更新日期：2018-10-01 00:00:00

abstract：OBJECTIVES:To engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool. RESULTS:Expression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae. Downregulation of ERG9 gene, that encodes squalene synthase, by replacing its nat...

journal_title：Biotechnology letters

authors： Amiri P,Shahpiri A,Asadollahi MA,Momenbeik F,Partow S

更新日期：2016-03-01 00:00:00

abstract：:Aptamers against inactive Vibrio alginolyticus were selected from an 82-nt ssDNA random library by systematic evolution of ligands by exponential enrichment. After 15 rounds of selection, the final pool of aptamers was highly specific for inactivated V. alginolyticus and had a dissociation constant of 27.5 ± 9.2 nM. U...

journal_title：Biotechnology letters

authors： Tang X,Zheng J,Yan Q,Li Z,Li Y

更新日期：2013-06-01 00:00:00

abstract：:An extracellular chitinase from Streptomyces halstedii AJ-7, a broad spectrum antifungal biocontrol agent, was characterized and purified. The apparent molecular weight of the purified protein was 55 kDa, Km value and Vmax of the protein for colloidal chitin were 3.2 mg ml(-1) and 118 micromol h(-1), respectively. The...

journal_title：Biotechnology letters

authors： Joo GJ

更新日期：2005-10-01 00:00:00

abstract：:Gene therapy is based on the principle of the genetic manipulation of DNA or RNA for treating and preventing human diseases. The clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease9 (CRISPR/Cas9) system, derived from the acquired immune system in bacteria and archaea, has provided a ne...

journal_title：Biotechnology letters

authors： Mollanoori H,Teimourian S

更新日期：2018-06-01 00:00:00