Abstract:
:Peptides are emerging as pharmaceutical agents in cancer therapy. The peptide, TLSGAFELSRDK (TLS) is a targeting ligand that can specifically triggers cellular uptake by binding to SKOV3 cells. Cell surface proteins and the C-terminal basic residues of the TLS are required for effective cell penetration, and the uptake process is energy-dependent. It inhibited the proliferation of SKOV3 cells and induced early-stage apoptosis by down-regulation of Bcl-2 expression mediated through a caspase-dependent pathway. The synergistic anti-proliferative effects of the peptide TLS and doxorubicin on SKOV3 cells were further investigated. Taken together, TLS, acting as a combination of a targeted ligand and a therapeutic agent, was a promising candidate for the development of peptide-based therapies in ovarian cancer.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Ma C,Yin G,You F,Wei Y,Huang Z,Chen X,Yan Ddoi
10.1007/s10529-013-1263-xsubject
Has Abstractpub_date
2013-11-01 00:00:00pages
1791-7issue
11eissn
0141-5492issn
1573-6776journal_volume
35pub_type
杂志文章abstract::The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1709-9
更新日期:2015-03-01 00:00:00
abstract::Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9583-3
更新日期:2008-04-01 00:00:00
abstract::Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0150-y
更新日期:2010-02-01 00:00:00
abstract:OBJECTIVES:To investigate antioxidative and biotransformation enzyme responses in Mucor hiemalis towards cyanotoxins considering its use in mycoremediation applications. RESULTS:Catalase (CAT), glutathione reductase (GR), and glutathione peroxidase (GPx) in M. hiemalis maintained their activities at all tested microcy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2348-8
更新日期:2017-08-01 00:00:00
abstract::The optimal process for the polysialylation reaction was as follows: polysialicacid (PSA) was activated by periodate oxidation, then coupled to CuZn superoxide dismutase (SOD) with a PSA:SOD molar ratio of 40:1 for 24 h. The resulting polysialylated protein contained 3.9 ± 0.3 mol PSA per mol SOD. SDS-PAGE and atomic ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0382-x
更新日期:2010-12-01 00:00:00
abstract:OBJECTIVES:To improve the efficiency, reproducibility and consistency of the PEI-based transfection method that is often used in preparation of recombinant lentiviral or retroviral vectors. RESULTS:The contributions to transfection efficiency of multi-factors including concentration of PEI or DNA, dilution buffer for ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2123-2
更新日期:2016-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0101-7
更新日期:2009-12-01 00:00:00
abstract:OBJECTIVES:To provide a simple method to make a stable ΔF508-CFTR-expressing T84 cell line that can be used as an efficient screening model system for ΔF508-CFTR rescue. RESULTS:CFTR knockout cell lines were generated by Cas9 with a single-guide RNA (sgRNA) targeting exon 1 of the CFTR genome, which produced indels th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2190-4
更新日期:2016-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9841-z
更新日期:2009-01-01 00:00:00
abstract::Ligation-mediated-PCR was performed followed by the mapping of 177 and 150 integration sites from HepG2 and Hek293 transduced with chimera vector carrying recombinant human Factor IX (rhFIX) cDNA, respectively. The sequences were analyzed for chromosome preference, CpG, transcription start site (TSS), repetitive eleme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1764-2
更新日期:2015-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2544-9
更新日期:2005-04-01 00:00:00
abstract::Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1032-2
更新日期:2012-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2516-5
更新日期:2018-04-01 00:00:00
abstract::Rhodococcus sp. CGMCC 4911 transformed 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives into corresponding diols. Ethylene sulfate, glycol sulfide, 1,3-PDS, and 1,2-propanediol cyclic sulfate were effectively hydrolyzed with growing cells. (R)-1,2-Propanediol (>99 % e.e.) was obtained at 44 % yield with gr...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1670-7
更新日期:2015-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9420-8
更新日期:2007-10-01 00:00:00
abstract::Alkyl glucosides were synthesized by the reaction of Leuconostoc mesenteroides dextransucrase with sucrose and various alcohols. Alkyl alpha-D-glucosides were obtained with a yield of 30% (mol/mol) with primary alcohols, but secondary alcohols or tertiary alcohols gave yields below 5%. The optimal yield was 50% using ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0015-4
更新日期:2009-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022398917213
更新日期:2003-02-01 00:00:00
abstract:PURPOSE OF WORK:The purpose of this study was to determine if Arabidopsis protoplast transfection could be scaled up, from the commonly used cell-based studies, to be used in triterpenoid production assays as an in planta alternative/complement to other expression systems. Enzyme activities are often identified using h...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1427-8
更新日期:2014-04-01 00:00:00
abstract::Clostridium spp. produce n-butanol in the acetone/butanol/ethanol process. For sustainable industrial scale butanol production, a number of obstacles need to be addressed including choice of feedstock, the low product yield, toxicity to production strain, multiple-end products and downstream processing of alcohol mixt...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-012-0926-3
更新日期:2012-08-01 00:00:00
abstract::An extracellular chitinase from Streptomyces halstedii AJ-7, a broad spectrum antifungal biocontrol agent, was characterized and purified. The apparent molecular weight of the purified protein was 55 kDa, Km value and Vmax of the protein for colloidal chitin were 3.2 mg ml(-1) and 118 micromol h(-1), respectively. The...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-1315-y
更新日期:2005-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2424-0
更新日期:2017-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1816-2
更新日期:2015-07-01 00:00:00
abstract::Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8655-5
更新日期:2005-08-01 00:00:00
abstract:OBJECTIVE:The growth characteristics and intracellular hemin concentrations of Lactococcus lactis grown under different culture pH and aeration conditions were examined to investigate the effect of culture pH on the respiration efficiency of L. lactis NZ9000 (pZN8148). RESULTS:Cell biomass and biomass yield of L. lact...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1999-6
更新日期:2016-03-01 00:00:00
abstract::A mutant ofS. chrysomallus with a D-glucose isomerase inducible by D-glucose or its catabolites was characterized. In contrast to the wild-type strain it showed a decreased catabolite repression by D-glucose of D-xylose consumption and a constitutive pentose phosphate pathway as well. A hypothesis concerning altered i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/BF01026199
更新日期:1987-12-01 00:00:00
abstract::The use of microRNAs (miRNAs) for improving the efficiency of recombinant protein production by CHO cells is gaining considerable interest for their ability to regulate entire molecular networks. Differential miRNA expression profiling and large-scale transient screening have been the prerequisite for the selection of...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1751-7
更新日期:2015-04-01 00:00:00
abstract::Tributyl phosphate (TBP) is widely used in nuclear fuel processing and other waste generating chemical industries. Although TBP is bacteriostatic, some microbes are resistant to it and may degrade it. Under dark aerobiosis, purple non-sulfur photosynthetic bacteria degraded up to 0.6 mM TBP, initially present at 2 mM,...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2882-7
更新日期:2005-04-01 00:00:00
abstract::Therapies for high-grade gliomas (HHG) that have strong tendency of infiltration and resistance to chemotherapies are currently unavailable. Here, we report that lower-dose combination therapy of Buthus martensii Karsch (BmK) CT, a type of scorpion toxin peptide, and LiCl, clinically used as mo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0741-2
更新日期:2012-01-01 00:00:00
abstract:OBJECTIVE:To engineer Pichia pastoris for heterologous production of cellulase from Musca domestica and explore its potential for industrial applications. RESULTS:A new beta-glucosidase gene (bg), encoding 562 amino acids, was cloned from M. domestica by using rapid amplification of cDNA ends. The gene bg was linked t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2351-0
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:Silk sericin is a natural polymer with potential utility in biomedical and biotechnological applications. Recombinantly expressed sericin ensures a source of pure protein with no contamination and with multiple properties when expressed as a fusion protein. Hence, the present paper aims to recombinantly expre...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-02911-3
更新日期:2020-09-01 00:00:00