Recombinant expression of sericin-cecropin fusion protein and its functional activity.

abstract：:A novel method to examine the morphology and structure of fungal hyphae in solid pastes used for the production of meat alternative product is presented. A sample of fermentation broth was fluorescently stained with Calcofluor White and added back to the broth, mixed and then a paste made using ultra-filtration. Fibre...

journal_title：Biotechnology letters

authors： Miri T,Cox PW,Fryer PJ

更新日期：2003-02-01 00:00:00

abstract：PURPOSE OF WORK:The purpose of this study was to determine if Arabidopsis protoplast transfection could be scaled up, from the commonly used cell-based studies, to be used in triterpenoid production assays as an in planta alternative/complement to other expression systems. Enzyme activities are often identified using h...

journal_title：Biotechnology letters

authors： Johnson EE,Jetter R,Wasteneys G

更新日期：2014-04-01 00:00:00

abstract：:Classical swine fever (CSF) is one of the most important viral diseases of swine worldwide. Although live or attenuated virus vaccines have been used to control CSFV, it is difficult to distinguish vaccinated pigs from infected pigs; this leads to restrictions on import and export. Subunit vaccines based on the CSFV E...

journal_title：Biotechnology letters

authors： Park Y,Lee S,Kang H,Park M,Min K,Kim NH,Gu S,Kim JK,An DJ,Choe S,Sohn EJ

更新日期：2020-07-01 00:00:00

abstract：:N(omega)-2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl (N(omega)-Pbf)-protected oligoarginine was directly conjugated to poly(amino acid) derivatives modified with a long alkyl chain. The final concentration of conjugated peptides was easily controlled by the feed ratio of oligoarginine to polymer backbone and a f...

journal_title：Biotechnology letters

authors： Yang SR,Kim SB,Joe CO,Kim JD

更新日期：2005-07-01 00:00:00

abstract：:Three previously established cell lines (yellow, red and white) of Saussurea medusa were investigated for jaceosidin and hispidulin production. Maximum yields of the jaceosidin and hispidulin were obtained in the red cell line at 75+/-0.41 and 6.4+/-0.31 mg l-1. Production of jaceosidin and hispidulin correlated with ...

journal_title：Biotechnology letters

authors： Fu CX,Zhao DX,Huang Y,Ma FS

更新日期：2005-01-01 00:00:00

abstract：:Laccases play an important role in the biological break down of lignin and have great potential in the deconstruction of lignocellulosic feedstocks. We examined 16 laccases, both commercially prepared and crude extracts, for their ability to oxidize veratryl alcohol in the presence of various solvents and mediators. S...

journal_title：Biotechnology letters

authors： Larson TM,Anderson AM,Rich JO

更新日期：2013-02-01 00:00:00

abstract：:L-threo-3,4-Dihydroxyphenylserine (DOPS) is a chiral unnatural beta-hydroxy amino acid used for the treatment of Parkinson disease. We developed a continuous bioconversion system for DOPS production that uses whole-cell biocatalyst of recombinant Escherichia coli expressing L-threonine aldolase (L-TA) genes cloned fro...

journal_title：Biotechnology letters

authors： Baik SH,Yoshioka H

更新日期：2009-03-01 00:00:00

abstract：:A novel glucose-sensing molecule was created based on galactose/glucose-binding protein (GGBP). GGBP mutants at Asp14, a residue interacting with the 4th hydroxyl group of the sugar molecule, were constructed by mutagenesis to improve the ligand specificity of GGBP. The autofluorescence-based analysis of the binding a...

journal_title：Biotechnology letters

authors： Sakaguchi-Mikami A,Taneoka A,Yamoto R,Ferri S,Sode K

更新日期：2008-08-01 00:00:00

abstract：:The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene su...

journal_title：Biotechnology letters

authors： Lim HA,Raku T,Tokiwa Y

更新日期：2005-04-01 00:00:00

abstract：OBJECTIVE:To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations RESULTS:Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl...

journal_title：Biotechnology letters

authors： Liu C,Li LJ,Wu CY,Guo KN,Li JH

更新日期：2016-07-01 00:00:00

abstract：:A mutant ofS. chrysomallus with a D-glucose isomerase inducible by D-glucose or its catabolites was characterized. In contrast to the wild-type strain it showed a decreased catabolite repression by D-glucose of D-xylose consumption and a constitutive pentose phosphate pathway as well. A hypothesis concerning altered i...

journal_title：Biotechnology letters

authors： Hänel F,Roth M,Neigenfind M,Gräfe U,Bormann EJ

更新日期：1987-12-01 00:00:00

abstract：:The hybridoma cell line, HB-8696, produces a monoclonal antibody, 520C9 (mouse IgG(1)) that recognizes the breast cancer oncoprotein, c-erbB2. The effect of perfusion rate (volume of fresh feed/working volume of reactor/day) on cell growth and mAb production was investigated but perfusion at a constant rate and at an ...

journal_title：Biotechnology letters

authors： Sen S,Roychoudhury PK

更新日期：2013-02-01 00:00:00

abstract：OBJECTIVE:Chinese hamster ovary (CHO) cells are the leading cell factories for producing recombinant proteins in the biopharmaceutical industry. In this regard, constraint-based metabolic models are useful platforms to perform computational analysis of cell metabolism. These models need to be regularly updated in order...

journal_title：Biotechnology letters

authors： Fouladiha H,Marashi SA,Li S,Li Z,Masson HO,Vaziri B,Lewis NE

更新日期：2021-01-01 00:00:00

abstract：:Two ketoreductases from Candida glabrata were used for the asymmetric reduction of prochiral substituted acetophenones displayed different enantiopreference toward para-, meta-substituted and ortho-halogen substituted acetophenones with excellent enantioselectivity. Homology modeling and docking analysis were in confo...

journal_title：Biotechnology letters

authors： Liang P,Qin B,Mu M,Zhang X,Jia X,You S

更新日期：2013-09-01 00:00:00

abstract：OBJECTIVES:Enhancement of the potential ability of biomacromolecules to cross cell membranes is a critical step for development of effective therapeutic vaccine especially DNA vaccine against human immunodeficiency virus-1 (HIV-1) infection. The supercharged proteins were known as powerful weapons for delivery of diffe...

journal_title：Biotechnology letters

authors： Kardani K,Bolhassani A,Agi E,Hashemi A

更新日期：2020-10-01 00:00:00

abstract：:A purified recombinant enzyme from Spirochaeta thermophila, that is suggested to be a cellobiose 2-epimerase, was a 47 kDa monomer with a specific activity of 29.2 U min(-1) for mannobiose. The epimerization activity of the recombinant enzyme for mannobiose was maximal at pH 7.0 and 60 °C with a half-life of 124 h. Th...

journal_title：Biotechnology letters

authors： Park CS,Kim JE,Lee SH,Kim YS,Kang LW,Oh DK

更新日期：2013-11-01 00:00:00

abstract：:In vitro synthesized mRNAs are often used as calibrants in gene expression analysis. In the case of an analytical procedure for gene expression containing a process of reverse transcription such as microarray analysis, full-length mRNAs having a 3'-poly(A)+tail are required as calibrants. However, the preparation of f...

journal_title：Biotechnology letters

authors： Matsuo T,Obana E,Yamamoto T,Kakuhata R,Yamazaki N,Kataoka M,Baba Y,Hori T,Shinohara Y

更新日期：2009-02-01 00:00:00

abstract：OBJECTIVE:To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. RESULTS:Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate,...

journal_title：Biotechnology letters

authors： Shariati L,Modaress M,Khanahmad H,Hejazi Z,Tabatabaiefar MA,Salehi M,Modarressi MH

更新日期：2016-08-01 00:00:00

abstract：:The use of microRNAs (miRNAs) for improving the efficiency of recombinant protein production by CHO cells is gaining considerable interest for their ability to regulate entire molecular networks. Differential miRNA expression profiling and large-scale transient screening have been the prerequisite for the selection of...

journal_title：Biotechnology letters

authors： Kelly PS,Gallagher C,Clynes M,Barron N

更新日期：2015-04-01 00:00:00

abstract：:A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of si...

journal_title：Biotechnology letters

authors： Pöhnlein M,Finkbeiner T,Syldatk C,Hausmann R

更新日期：2015-03-01 00:00:00

abstract：:A new technique for the direct production of recombinant baculovirus in the silkworm larvae is described. To assess the utility of this method, a combination of Bombyx mori nucleopolyhedroviral genome, transfer vector and Lipofectin was co-injected directly into newly ecdysed fifth instar, silkworm larvae. The recombi...

journal_title：Biotechnology letters

authors： Lee KS,Kim BY,Je YH,Woo SD,Sohn HD,Jin BR

更新日期：2007-01-01 00:00:00

abstract：:Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...

journal_title：Biotechnology letters

authors： Li Y

更新日期：2011-08-01 00:00:00

abstract：:Three biological activities of 12 soy isoflavones were investigated in terms of the structure-activity relationship. Seven molecular descriptors and one absorption predictor were significantly related to the inhibition of cytochrome P4501 activity in HepG2 cells by soy isoflavones, yielding water solubility [correlati...

journal_title：Biotechnology letters

authors： Chun HS,Chang HJ,Choi EH,Kim HJ,Ku KH

更新日期：2005-08-01 00:00:00

abstract：:Environmental factors, such as drought, salinity, extreme temperature, ozone poisoning, metal toxicity etc., significantly affect crops. To study these factors and to design a possible remedy, biological experimental data concerning these crops requires the quantification of gene expression and comparative analyses at...

journal_title：Biotechnology letters

authors： Gul A,Ahad A,Akhtar S,Ahmad Z,Rashid B,Husnain T

更新日期：2016-04-01 00:00:00

abstract：:Organophosphorus hydrolase (OPH) hydrolyzes organophosphorus esters. We constructed the yeast-displayed OPH using Flo1p anchor system. In this system, the N-terminal region of the protein was fused to Flo1p and the fusion protein was displayed on the cell surface. Hydrolytic reactions with paraoxon were carried out du...

journal_title：Biotechnology letters

authors： Fukuda T,Tsuchiyama K,Makishima H,Takayama K,Mulchandani A,Kuroda K,Ueda M,Suye S

更新日期：2010-05-01 00:00:00

abstract：OBJECTIVES:To investigated the potential of a novel dendrosomal nanoformulation of curcumin (DNC) in blocking radiation-induced changes in irradiated human umbilical vein endothelial cells (HUVECs), and their adhesion to human THP-1 monocytoid cells. RESULTS:Co60 gamma rays reduced viability, raised the expression of ...

journal_title：Biotechnology letters

authors： Soltani B,Bodaghabadi N,Mahpour G,Ghaemi N,Sadeghizadeh M

更新日期：2016-12-01 00:00:00

abstract：:Ganoderic acid, from Ganoderma lucidum, at 8 microg/ml inhibited replication of hepatitis B virus (HBV) in HepG2215 cells over 8 days. Production of HBV surface antigen and HBV e antigen were 20 and 44% of controls without ganoderic acid. Male KM mice were significantly protected from liver injury, induced with carbon...

journal_title：Biotechnology letters

authors： Li YQ,Wang SF

更新日期：2006-06-01 00:00:00

abstract：:The ion-exchange chromatography behavior of recombinant glucose dehydrogenase harboring pyrroloquinoline quinone (PQQGDH) was modified to greatly simplify its purification. The surface charge of PQQGDH was engineered by either fusing a three-arginine tail to the C-terminus of PQQGDH (PQQGDH+Arg3) or by substituting th...

journal_title：Biotechnology letters

authors： Koh H,Igarashi S,Sode K

更新日期：2003-10-01 00:00:00

abstract：:Three chloroplast genes, chlL, chlN and chlB, encoding the light-independent protochlorophyllide oxidoreductase (LIPOR) in Chlorella protothecoides CS-41 growing either photoautotrophically, mixotrophically or heterotrophically, were all transcribed constitutively independent of illumination and presence of glucose. S...

journal_title：Biotechnology letters

authors： Shi C,Shi X

更新日期：2006-02-01 00:00:00

abstract：OBJECTIVES:To exploit cold-inducible biochemical processes beneficial for foreign mRNA transcription, translation and storage, as well as protein product stability, during Agrobacterium-mediated transient expression. RESULTS:The efficiency of three different 5'-regulatory sequences to achieve transient expression of t...

journal_title：Biotechnology letters

authors： Gerasymenko IM,Sheludko YV

更新日期：2017-07-01 00:00:00