Improvement in organophosphorus hydrolase activity of cell surface-engineered yeast strain using Flo1p anchor system.

abstract：:Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 degrees C, and Lac II was at pH 4.2 and 50 degrees C...

journal_title：Biotechnology letters

authors： Garcia TA,Santiago MF,Ulhoa CJ

更新日期：2006-05-01 00:00:00

abstract：:N(omega)-2,2,4,6,7-Pentamethyldihydrobenzofuran-5-sulfonyl (N(omega)-Pbf)-protected oligoarginine was directly conjugated to poly(amino acid) derivatives modified with a long alkyl chain. The final concentration of conjugated peptides was easily controlled by the feed ratio of oligoarginine to polymer backbone and a f...

journal_title：Biotechnology letters

authors： Yang SR,Kim SB,Joe CO,Kim JD

更新日期：2005-07-01 00:00:00

abstract：OBJECTIVE:To construct effective genetic expression parts controlling transcription and translation initiation for synthetic biology and heterologous expression in Corynebacterium glutamicum. RESULT:Twelve highly expressed genes were identified from the proteomic data of C. glutamicum. Their related sequences were use...

journal_title：Biotechnology letters

authors： Zhao Z,Liu X,Zhang W,Yang Y,Dai X,Bai Z

更新日期：2016-12-01 00:00:00

abstract：:Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...

journal_title：Biotechnology letters

authors： Turner KM,Pasut G,Veronese FM,Boyce A,Walsh G

更新日期：2011-03-01 00:00:00

abstract：:With ever-increasing molecular information about colorectal cancer (CRC), there is an expectation to detect more sensitive and specific molecular markers for new advanced diagnostic methods that can surpass the limitations of current screening tests. Moreover, enhanced molecular pathology knowledge about cancer has le...

journal_title：Biotechnology letters

authors： Kavousipour S,Khademi F,Zamani M,Vakili B,Mokarram P

更新日期：2017-06-01 00:00:00

abstract：:The production of a low-temperature alkalophilic lipase from Acinetobacter johnsonii was improved using genome shuffling. The starting populations, obtained by UV irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. The optimal conditions for protoplast formation and regeneration...

journal_title：Biotechnology letters

authors： Wang H,Zhang J,Wang X,Qi W,Dai Y

更新日期：2012-01-01 00:00:00

abstract：:Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l(-1), whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l(-1). The particle median diameter decreased from 2 to 0.5 mm...

journal_title：Biotechnology letters

authors： Lucatero S,Galindo E,Larralde-Corona CP

更新日期：2004-01-01 00:00:00

abstract：OBJECTIVES:An extracellular protease inhibitor (BTPI-301) of trypsin was purified and characterized from an isolate of Pseudomonas mendocina. RESULTS:BTPI-301was purified to homogeneity by (NH4)2SO4, precipitation, DEAE Sepharose and CNBr-activated Sepharose chromatography. Homogeneity was proved by native PAGE and SD...

journal_title：Biotechnology letters

authors： Sapna K,Manzur Ali PP,Rekha Mol KR,Bhat SG,Chandrasekaran M,Elyas KK

更新日期：2017-12-01 00:00:00

abstract：:Avian myeloblastosis virus reverse transcriptase (AMV RT) is a heterodimer consisting of a 63 kDa α-subunit and a 95 kDa β subunit. Moloney murine leukaemia virus reverse transcriptase (MMLV RT) is a 75 kDa monomer. These two RTs are the most extensively used for conversion of RNA to DNA. We previously developed sever...

journal_title：Biotechnology letters

authors： Konishi A,Yasukawa K,Inouye K

更新日期：2012-07-01 00:00:00

abstract：OBJECTIVES:To investigate the effect of the linker region (LR) on the enzymatic activity, stability, and flexibility of Bacillus subtilis cellulase CelI15, six mutants were constructed that contained increasing numbers of the LR. RESULTS:The CelI15 mutant with three copies of the LR (approx. 57 amino acids) showed the...

journal_title：Biotechnology letters

authors： Li P,Zhou Y,Li Q,Zhang C,Sun Z,Tian L,Weng H

更新日期：2016-09-01 00:00:00

abstract：:beta-Galactosidase isolated from Aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 U g(-1)) giving 32% of its original activity. Immobilization did not change the pH optimum (4.5) of lactose hydrolysis. The relative enzyme activity during product inhibition testing was, in a...

journal_title：Biotechnology letters

authors： Grosová Z,Rosenberg M,Rebros M,Sipocz M,Sedlácková B

更新日期：2008-04-01 00:00:00

abstract：:The recombinant laccase from Thermus thermophilus was applied to the biobleaching of wheat straw pulp. The best bleaching effect was when the pulp was treated with 3 U laccase g(-1) dry pulp at 90°C, pH 4.5, 8% consistency for 1.5 h. Under these conditions, the pulp brightness was increased by 3.3% ISO, and the pulp k...

journal_title：Biotechnology letters

authors： Zheng Z,Li H,Li L,Shao W

更新日期：2012-03-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The dete...

journal_title：Biotechnology letters

authors： Qiao YM,Guo YC,Zhang XE,Zhou YF,Zhang ZP,Wei HP,Yang RF,Wang DB

更新日期：2007-12-01 00:00:00

abstract：:The genetics governing the morphological switch from round or ovoid cells to filamentous growth in Saccharomyces cerevisiae has received significant interest in relation to sensing and signaling pathways as well as the control of cell processes including budding, elongation and adhesion. Little is known about the envi...

journal_title：Biotechnology letters

authors： Ceccato-Antonini SR

更新日期：2008-07-01 00:00:00

abstract：:Fourteen strains of Bacillus thuringiensis collected from both larvae showing disease symptoms and soil samples in northwest Argentina were characterized by insecticidal activity against Spodoptera frugiperda. First instar larvae and protein profile SDS-PAGE analysis of whole cell proteins not only allowed the differe...

journal_title：Biotechnology letters

authors： Alvarez A,Pera LM,Loto F,Virla EG,Baigori MD

更新日期：2009-01-01 00:00:00

abstract：:Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) c...

journal_title：Biotechnology letters

authors： Chung CP,Jang JH

更新日期：2005-06-01 00:00:00

abstract：:There is a great need for the development of biomimetic human tissue models that allow elucidation of the pathophysiological conditions involved in disease initiation and progression. Conventional two-dimensional (2D) in vitro assays and animal models have been unable to fully recapitulate the critical characteristics...

journal_title：Biotechnology letters

authors： Memic A,Navaei A,Mirani B,Cordova JAV,Aldhahri M,Dolatshahi-Pirouz A,Akbari M,Nikkhah M

更新日期：2017-09-01 00:00:00

abstract：:The thermostablility and enzymatic activity of 1,3-1,4-β-glucanase (BglA) from Bacillus amyloliquefaciens was improved by modifying five (out of 12) ε-amino groups in lysine residues with nitrous acid. The optimal modification condition for BglA was determined as 30 mM nitrous acid at, 40 °C for 30 min. The optimally-...

journal_title：Biotechnology letters

authors： Niu C,Zhu L,Wang J,Li Q

更新日期：2014-12-01 00:00:00

abstract：:1,3-Propanediol production by Klebsiella pneumoniae was studied in batch cultures under N2 flow and four airflow systems. Different byproducts were formed under different aeration conditions. An anaerobic/aerobic combined fed-batch culture was developed giving 70 g 1,3-propanediol l(-1) and 16 g 2,3-butanediol l(-1) w...

journal_title：Biotechnology letters

authors： Cheng KK,Liu DH,Sun Y,Liu WB

更新日期：2004-06-01 00:00:00

abstract：:The addresses given for the authors of this paper were incorrect. The correct assignments of the authors are given in this erratum. ...

journal_title：Biotechnology letters

authors： Li W,Chen Y,Sheng Y,Xie Z,Tang Y

更新日期：2018-01-01 00:00:00

abstract：:At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances...

journal_title：Biotechnology letters

authors： Le H,Vishwanathan N,Jacob NM,Gadgil M,Hu WS

更新日期：2015-08-01 00:00:00

abstract：OBJECTIVES:Enhancement of the potential ability of biomacromolecules to cross cell membranes is a critical step for development of effective therapeutic vaccine especially DNA vaccine against human immunodeficiency virus-1 (HIV-1) infection. The supercharged proteins were known as powerful weapons for delivery of diffe...

journal_title：Biotechnology letters

authors： Kardani K,Bolhassani A,Agi E,Hashemi A

更新日期：2020-10-01 00:00:00

abstract：:The substrate specificity of alpha-chymotrypsin and other serine proteases, trypsin, elastase, proteinase K and subtilisin, towards hydrolysis of various polyesters was examined using poly(L-lactide) (PLA), poly(beta-hydroxybutyrate) (PHB), poly(ethylene succinate) (PES), poly(ethylene adipate) (PEA), poly(butylene su...

journal_title：Biotechnology letters

authors： Lim HA,Raku T,Tokiwa Y

更新日期：2005-04-01 00:00:00

abstract：:The optimal process for the polysialylation reaction was as follows: polysialicacid (PSA) was activated by periodate oxidation, then coupled to CuZn superoxide dismutase (SOD) with a PSA:SOD molar ratio of 40:1 for 24 h. The resulting polysialylated protein contained 3.9 ± 0.3 mol PSA per mol SOD. SDS-PAGE and atomic ...

journal_title：Biotechnology letters

authors： Wu JR,Lin Y,Zheng ZY,Lin CC,Zhan XB,Shen YQ

更新日期：2010-12-01 00:00:00

abstract：:Genetically-coded, fluorescence resonance energy transfer (FRET) biosensors are widely used to study molecular events from single cells to whole organisms. They are unique among biosensors because of their spontaneous fluorescence and targeting specificity to both organelles and tissues. In this review, we discuss the...

journal_title：Biotechnology letters

authors： Li IT,Pham E,Truong K

更新日期：2006-12-01 00:00:00

abstract：:Seeds play essential roles in plant life cycle and germination is a complex process which is associated with different phases of water imbibition. Upon imbibition, seeds begin utilization of storage substances coupled with metabolic activity and biosynthesis of new proteins. Regeneration of organelles and emergence of...

journal_title：Biotechnology letters

authors： Zaynab M,Kanwal S,Furqan M,Islam W,Noman A,Ali GM,Rehman N,Zafar S,Sughra K,Jahanzab M

更新日期：2017-10-01 00:00:00

abstract：:An extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25°C. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. EstK was purified to homogeneit...

journal_title：Biotechnology letters

authors： Hong S,Lee C,Jang SH

更新日期：2012-06-01 00:00:00

abstract：:A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast...

journal_title：Biotechnology letters

authors： Takagi M,Kitabayashi T,Koizumi S,Hirose H,Kondo S,Fujiwara M,Ueno K,Hiroaki M,Hosokawa Y,Masuhara H,Wakitani S

更新日期：2008-07-01 00:00:00

abstract：:Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained...

journal_title：Biotechnology letters

authors： Noda K,Matsuno T,Fujii H,Kogure T,Urata M,Asami Y,Kuroda A

更新日期：2008-06-01 00:00:00

abstract：:A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated...

journal_title：Biotechnology letters

authors： Lugg H,Sammons RL,Marquis PM,Hewitt CJ,Yong P,Paterson-Beedle M,Redwood MD,Stamboulis A,Kashani M,Jenkins M,Macaskie LE

更新日期：2008-03-01 00:00:00