Extending the linker region increases the activity of the Bacillus subtilis cellulase CelI15.

Abstract:

OBJECTIVES:To investigate the effect of the linker region (LR) on the enzymatic activity, stability, and flexibility of Bacillus subtilis cellulase CelI15, six mutants were constructed that contained increasing numbers of the LR. RESULTS:The CelI15 mutant with three copies of the LR (approx. 57 amino acids) showed the highest activity, which was almost 20 % greater than that of wild type CelI15. The stability of the mutant enzymes increased as the copy number of the LR decreased. However, the substrate affinity of the mutant enzymes increased as the LR copy number increased, and the mutant with four copies of the LR exhibited the highest substrate affinity. Additionally, the flexibility of the CelI15 mutants increased as the LR copy number increased from zero to four copies, although it decreased sharply for the mutant with five copies of the LR. CONCLUSION:The activity of CelI15 was increased by increasing the LR copy number, which could be a potential way to improve its enzymatic properties.

journal_name

Biotechnol Lett

journal_title

Biotechnology letters

authors

Li P,Zhou Y,Li Q,Zhang C,Sun Z,Tian L,Weng H

doi

10.1007/s10529-016-2136-x

subject

Has Abstract

pub_date

2016-09-01 00:00:00

pages

1587-93

issue

9

eissn

0141-5492

issn

1573-6776

pii

10.1007/s10529-016-2136-x

journal_volume

38

pub_type

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