Abstract:
:A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The detection limits of the LAMP assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. The results show that the LAMP protocol is a promising method for detecting B. anthracis.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Qiao YM,Guo YC,Zhang XE,Zhou YF,Zhang ZP,Wei HP,Yang RF,Wang DBdoi
10.1007/s10529-007-9472-9subject
Has Abstractpub_date
2007-12-01 00:00:00pages
1939-46issue
12eissn
0141-5492issn
1573-6776journal_volume
29pub_type
杂志文章abstract:OBJECTIVES:To find a novel host for the production of 4-vinylphenol (4VPh) by screening Streptomyces species. RESULTS:The conversion of p-coumaric acid (pHCA) to 4VPh in Streptomyces mobaraense was evaluated using a medium containing pHCA. S. mobaraense readily assimilated pHCA after 24 h of cultivation to produce 4VP...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2126-z
更新日期:2016-09-01 00:00:00
abstract::The effects of the sequential application of methyl jasmonate (MeJa), salicylic acid (SA) and yeast extract (YE) to Argemone mexicana cell cultures were compared to either the sole application of each elicitor, or to the three-partite mixture. The highest sanguinarine accumulation occurred using the sequential treatme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0770-x
更新日期:2012-02-01 00:00:00
abstract::Three biological activities of 12 soy isoflavones were investigated in terms of the structure-activity relationship. Seven molecular descriptors and one absorption predictor were significantly related to the inhibition of cytochrome P4501 activity in HepG2 cells by soy isoflavones, yielding water solubility [correlati...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8457-9
更新日期:2005-08-01 00:00:00
abstract::δ-Integration can improve the expression stability and increase the copy number of exogenous genes in Saccharomyces cerevisiae. Generally, δ-integration vectors employ auxotrophic markers, such as LEU2-d, HIS3, TRP1, and URA3, to screen transformants. In this study, two cellulase genes (β-glucosidase and endoglucanase...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1205-7
更新日期:2013-08-01 00:00:00
abstract::The use of microRNAs (miRNAs) for improving the efficiency of recombinant protein production by CHO cells is gaining considerable interest for their ability to regulate entire molecular networks. Differential miRNA expression profiling and large-scale transient screening have been the prerequisite for the selection of...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1751-7
更新日期:2015-04-01 00:00:00
abstract::Brevibacterium epidermis DSM 20659 is a halotolerant Gram-positive bacterium which can synthesize the osmolyte, ectoine, but prefers to take it up from its environment. The present study revealed that B. epidermis is equipped with at least one transport system for ectoine, with a maximal transport velocity of 15.7 +/-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9149-9
更新日期:2006-11-01 00:00:00
abstract::Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D: -lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for bo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6585-2
更新日期:2005-01-01 00:00:00
abstract::MicroRNA (miRNA) profiling is a first important step in elucidating miRNA functions. Real time quantitative PCR (RT-qPCR) and microarray hybridization approaches as well as ultra high throughput sequencing of miRNAs (small RNA-seq) are popular and widely used profiling methods. All of these profiling approaches face s...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0380-z
更新日期:2010-12-01 00:00:00
abstract::Decrystallized chitosan was produced from shrimp shells with a low degree of crystallinity (10%) and a high anionic dye binding capacity. Raw, mixed dye wastewater from a textile factory was efficiently decolorized using decrystallized chitosan that was more efficient than using normal chitosan and activated carbon. D...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024562900548
更新日期:2003-07-01 00:00:00
abstract::O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0178-z
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVES:To engineer the yeast Saccharomyces cerevisiae for the heterologous production of linalool. RESULTS:Expression of linalool synthase gene from Lavandula angustifolia enabled heterologous production of linalool in S. cerevisiae. Downregulation of ERG9 gene, that encodes squalene synthase, by replacing its nat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-2000-4
更新日期:2016-03-01 00:00:00
abstract::Fourteen yeast strains were screened for production of 2-phenylethanol from L-phenylalanine with molasses as carbon source. Up to 1 g 2-phenylethanol l-1 was obtained. Using oleyl alcohol as a second phase for in situ product removal to enhance the production of 2-phenylethanol increased the yield to about 3 g 2-pheny...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022890119847
更新日期:2003-04-01 00:00:00
abstract::The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0720-7
更新日期:2011-12-01 00:00:00
abstract::We report a novel and convenient method for the determination of glycoproteins, especially antibodies, using galactose oxidase (GAO) on the basis of the contents of galactosyl and N-acetylgalactosaminyl residues in carbohydrate chains of glycoproteins. GAO converts galactose residues to their corresponding aldehyde an...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9102-y
更新日期:2006-09-01 00:00:00
abstract::Human phospholipid scramblase 1 (hPLSCR1) scrambles plasma membrane phospholipids during cell activation, blood coagulation and apoptosis. It was over-expressed in E. coli with a histidine tag and purified from the inclusion bodies (*30 mg/l culture broth) under denaturing conditions using 8 M urea. The denatured hPLS...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9797-z
更新日期:2008-12-01 00:00:00
abstract::Human hemangioblasts exist only during the early embryonic developmental stage thereby limiting the adult cellular source from which to obtain such cells for study. To overcome this, hemangioblast studies have focused on utilizing human embryonic stem cell (hESC) derivatives but current methods are cell-line dependent...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1786-4
更新日期:2015-06-01 00:00:00
abstract::The lipase-catalyzed reaction is useful to obtain sugar esters with chemically defined structures and will contribute to the synthesis of sugar-based compounds by a chemo-enzymatic pathway. The synthesis of sugar esters in nonaqueous media has been attempted for a quarter century. To facilitate the reactions, they hav...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0663-z
更新日期:2011-10-01 00:00:00
abstract:OBJECTIVES:To improve the efficiency, reproducibility and consistency of the PEI-based transfection method that is often used in preparation of recombinant lentiviral or retroviral vectors. RESULTS:The contributions to transfection efficiency of multi-factors including concentration of PEI or DNA, dilution buffer for ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2123-2
更新日期:2016-09-01 00:00:00
abstract::General methods for identification of usual carotenoids including C18-HPLC retention time and spectroscopic methods, such as absorption spectra, mass spectra, NMR spectra, are briefly summarized. ...
journal_title:Biotechnology letters
pub_type: 信件
doi:10.1007/s10529-014-1479-4
更新日期:2014-06-01 00:00:00
abstract::Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-8655-5
更新日期:2005-08-01 00:00:00
abstract::Our current knowledge of protein unfolding is overwhelmingly related to reversible denaturation. However, to engineer thermostable enzymes for industrial applications and medical diagnostics, it is necessary to consider irreversible denaturation processes and/or the entire quaternary structure. In this study we have u...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3555-2
更新日期:2005-11-01 00:00:00
abstract::A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5794-7
更新日期:2005-06-01 00:00:00
abstract::An unstructured model based on mass balance equations for a recombinant methylotrophic yeast Pichia pastoris MutS (Methanol Utilization Slow) strain expressing the mini-proinsulin (MPI), was successfully established in quasi-steady state fed-batch fermentations with varying total quantity of biomass in a 7 l fermenter...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022398917213
更新日期:2003-02-01 00:00:00
abstract::Three chloroplast genes, chlL, chlN and chlB, encoding the light-independent protochlorophyllide oxidoreductase (LIPOR) in Chlorella protothecoides CS-41 growing either photoautotrophically, mixotrophically or heterotrophically, were all transcribed constitutively independent of illumination and presence of glucose. S...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5528-x
更新日期:2006-02-01 00:00:00
abstract::The gene encoding Schwanniomyces occidentalis alpha-amylase (AMY) was introduced into the chromosomal delta sequences of an industrial strain of Saccharomyces cerevisiae. To obtain a strain suitable for commercial use, an delta-integrative cassette devoid of bacterial DNA sequences was constructed that contains the AM...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1026281627466
更新日期:2003-11-01 00:00:00
abstract::A morphological parameter of polygonal index was defined as the ratio of cell adhesion area versus the square of the major cell axis, and cells that had an adhesion area larger than 4000 mum(2) and a polygonal index larger than 0.3 were considered large polygonal cells. Cell morphology tended to change from fibroblast...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9683-8
更新日期:2008-07-01 00:00:00
abstract::A recombinant baculovirus was constructed to simultaneously express codon-optimized virus-like particles (VLP), A VP1-2A-VP3 and VP0 of serotype O foot-and-mouth disease virus (FMDV), from individual promoters. The target proteins were expressed in insect cells at high level, as shown by indirect sandwich ELISA; and t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0295-8
更新日期:2010-09-01 00:00:00
abstract::Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9621-1
更新日期:2008-05-01 00:00:00
abstract::A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9561-9
更新日期:2008-03-01 00:00:00
abstract::At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1843-z
更新日期:2015-08-01 00:00:00