Abstract:
:Recombinant human interleukin-1 receptor antagonist (rHuIL-1ra) was produced in E. coli as an inclusion body. rHuIL-1ra was purified to Over 98% purity by anion exchange chromatography after on-column refolding. The optimized processes produced more than 2 g pure refolded rHuIL-1ra per 1 l culture, corresponding to a 44% recovery, without an intermediate dialysis step. Refolded rHuIL-1ra had full biological activity with the MTT assay. An intramolecular disulfide linkage in the oxidized recombinant protein was suggested by data from HPLC and non-reducing SDS-PAGE.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Tan H,Dan G,Gong H,Cao Ldoi
10.1007/s10529-005-8655-5keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
1177-82issue
16eissn
0141-5492issn
1573-6776journal_volume
27pub_type
杂志文章abstract:OBJECTIVES:To study the roles and mechanisms of HuR in cancer stem cell maintenance of lung cancer. RESULTS:HuR expression was increased in tumor spheres of lung cancer cells. Knockdown of HuR suppressed spheroid formation and size, inhibited the expression of stemness-related marker, Oct4, Nanog and ALDH in lung canc...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2512-9
更新日期:2018-04-01 00:00:00
abstract::Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021750719337
更新日期:2003-01-01 00:00:00
abstract::Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O(2) transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9949-9
更新日期:2009-06-01 00:00:00
abstract::Decrystallized chitosan was produced from shrimp shells with a low degree of crystallinity (10%) and a high anionic dye binding capacity. Raw, mixed dye wastewater from a textile factory was efficiently decolorized using decrystallized chitosan that was more efficient than using normal chitosan and activated carbon. D...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024562900548
更新日期:2003-07-01 00:00:00
abstract:OBJECTIVES:To use molecular docking and dynamic simulation to investigate the inhibitory action of chlorogenic acid (CHA) and its analogues against sortase A of Staphylococcus aureus. RESULTS:Five novel, natural inhibitors with different activities were discovered for sortase A (SrtA). The inhibition mechanism of the ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2112-5
更新日期:2016-08-01 00:00:00
abstract::Two versions of an optimized gene that encodes human papilloma virus type 16 major protein L1 were designed according to the codon usage frequency of Pichia pastoris. Y16 was highly expressed in both P. pastoris and Hansenula polymorpha. M16 expression was as efficient as that of Y16 in P. pastoris, but merely detecta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1455-z
更新日期:2014-05-01 00:00:00
abstract::Trichodesmium sp., isolated from the Great Barrier Reef lagoon, was cultured in artificial seawater media containing a range of Fe concentration. Fe additions stimulated growth, N2 fixation, cellular chlorophyll a content, light-saturated chlorophyll a-specific gross photosynthetic capacity (Pmchl a) and the dark resp...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1023068232375
更新日期:2003-04-01 00:00:00
abstract::Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stab...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9659-8
更新日期:2008-06-01 00:00:00
abstract::A recombinant allophycocyanin (rAPC), used for treatment of tumors, has been expressed in E. coli which was grown in glucose fed-batch culture in a 30 l fermentor. Recombinant allophycocyanin was purified from soluble E. coli cell lysate using hydrophobic interaction chromatography followed by chromatography using amy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5794-7
更新日期:2005-06-01 00:00:00
abstract::The expression of secreted proteins in filamentous fungi is down-regulated by a transcriptional feedback mechanism under endoplasmic reticulum stress, termed repression under secretion stress (RESS). To investigate the RESS mechanism, we analyzed the expression of the Taka-amylase A gene (amyB) in Aspergillus oryzae, ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1691-2
更新日期:2015-02-01 00:00:00
abstract::The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022498314354
更新日期:2003-03-01 00:00:00
abstract::Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-0150-y
更新日期:2010-02-01 00:00:00
abstract::A dicistronic expression vector was constructed for Chinese hamster ovary (CHO) cells that produce both selectable marker-DHFR (dihydrofolate reductase) gene and recombinant antibody cDNA from a single primary transcript via differential splicing. The vector was derived from a pDHL vector and contained the human const...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2736-3
更新日期:2005-11-01 00:00:00
abstract::We assessed the whole genome amplification strategy, known as multiple displacement amplification (MDA), for use with the TaqMan genotyping platform for DNA samples derived from two case-control studies nested in the Nurses' Health Study and the Physicians' Health Study. Our objectives were to (1) quantify DNA yield f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1024173909401
更新日期:2003-07-01 00:00:00
abstract::Chlorella protothecoides, a lutein-producing microalga, was grown aerobically in a mineral medium prepared with 70% (v/v) deuterated water. HPLC/atmospheric pressure chemical ionization-mass spectrometry (HPLC/APCI-MS) analysis revealed approximately 58% replacement of hydrogen by deuterium atoms as indicated by the m...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9105-8
更新日期:2006-09-01 00:00:00
abstract::The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. Also the exposure of a calcium phosphate-DNA coprecipitat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9167-7
更新日期:2006-11-01 00:00:00
abstract::Biotechnology using molecular biology, biochemistry, biophysics, and computational approaches provides an alternative approach for classical pharmacological screening to look at ligand-receptor interactions and receptor specificity, which should support the design of selective drugs based on detailed structural princi...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-006-9005-y
更新日期:2006-06-01 00:00:00
abstract:OBJECTIVES:We examined the importance of aptamer usage under the same condition as the selection process by employing the previously selected aptamers for calmodulin (CaM) which includes a non-natural fluorogenic amino acid, 7-nitro-2,1,3-benzoxadiazole. RESULTS:We added five amino acids at the N-terminus which was em...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2257-2
更新日期:2017-03-01 00:00:00
abstract::A new ribonuclease (RNase) with tobacco mosaic virus inhibition was isolated and purified from Bacillus cereus ZH14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of DEAE-Sephadex A-50 column, and gel chromatography of Sephacryl S-200HR column. The enzyme was purified approximatel...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9831-1
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVE:The effects of a brief (3 days) and prolonged (6 days) period of incubation in darkness and light on the biomass content, lipid content and fatty acid profile in Chlorella vulgaris UMT-M1 were determined. RESULTS:Three days of incubation in darkness increased saturated fatty acid (SFA) content from 34.0 to 4...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-021-03077-2
更新日期:2021-01-12 00:00:00
abstract::beta-Galactosidase isolated from Aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 U g(-1)) giving 32% of its original activity. Immobilization did not change the pH optimum (4.5) of lactose hydrolysis. The relative enzyme activity during product inhibition testing was, in a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9606-0
更新日期:2008-04-01 00:00:00
abstract::General methods for identification of usual carotenoids including C18-HPLC retention time and spectroscopic methods, such as absorption spectra, mass spectra, NMR spectra, are briefly summarized. ...
journal_title:Biotechnology letters
pub_type: 信件
doi:10.1007/s10529-014-1479-4
更新日期:2014-06-01 00:00:00
abstract::Lymphotactin-XCL1 is a chemokine produced mainly by activated CD8+ T-cells and directs migration of CD4+ and CD8+ lymphocytes and natural killer (NK) cells. We expressed human lymphotactin (LTN) by the lactic-acid bacterium Lactococcus lactis. Biological activity of LTN was confirmed by chemo-attraction of human T-cel...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9855-6
更新日期:2009-02-01 00:00:00
abstract::Three chloroplast genes, chlL, chlN and chlB, encoding the light-independent protochlorophyllide oxidoreductase (LIPOR) in Chlorella protothecoides CS-41 growing either photoautotrophically, mixotrophically or heterotrophically, were all transcribed constitutively independent of illumination and presence of glucose. S...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5528-x
更新日期:2006-02-01 00:00:00
abstract::An on-line device, ORP (oxidation-reduction potential)-stat, was used to control glucose-feeding for enhancing xylitol conversion from D-xylose during an oxygen-limited fermentation by Candida tropicalis. The fermentation was carried out in a 5 l jar fermenter. After glucose in the medium was depleted, a switching to ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::Angiogenin, a potent angiogenic factor, was cloned and expressed by Escherichia coli and then purified with gel filtration chromatography. Approximately 90% pure angiogenin was obtained to generate a monoclonal antibody. Using western immunoblotting and ELISA, we confirmed that monoclonal antibody C46 secreted from hy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9651-3
更新日期:2008-06-01 00:00:00
abstract:OBJECTIVES:The promoter of HpMET3, encoding an ATP sulfurylase, was evaluated for its potential as a repressible promoter to downregulate the expression of target genes in the thermotolerant, methylotrophic yeast Hansenula polymorpha. RESULTS:The expression of lacZ under the control of the 0.6 kb HpMET3 promoter was e...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1902-5
更新日期:2015-11-01 00:00:00
abstract:OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02658-6
更新日期:2019-05-01 00:00:00
abstract::Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the human Abeta gene into the...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9759-5
更新日期:2008-10-01 00:00:00
abstract::The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1709-9
更新日期:2015-03-01 00:00:00