Abstract:
:The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. Also the exposure of a calcium phosphate-DNA coprecipitate to high osmolarity in vitro caused the release of the DNA from the precipitate. The results demonstrate the importance of the osmotic shock on the efficient delivery of plasmid DNA to the nucleus of CHO cells following calcium phosphate-mediated transfection.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Grosjean F,Bertschinger M,Hacker DL,Wurm FMdoi
10.1007/s10529-006-9167-7subject
Has Abstractpub_date
2006-11-01 00:00:00pages
1827-33issue
22eissn
0141-5492issn
1573-6776journal_volume
28pub_type
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2004-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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doi:10.1007/s10529-012-1021-5
更新日期:2012-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-010-0279-8
更新日期:2010-08-01 00:00:00
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更新日期:2015-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2014-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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doi:10.1023/a:1021750719337
更新日期:2003-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-005-0024-x
更新日期:2005-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9938-z
更新日期:2009-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03039-0
更新日期:2021-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2019-05-01 00:00:00