Abstract:
:Fermentation kinetics of Penicillium aculeatum ATCC 10409 demonstrated that fungal growth and dextranase release are decoupled. Inoculation by conidia or mycelia resulted in identical kinetics. Two new isoenzymes of the dextranase were characterized regarding their kinetic constants, pI, MW, activation energy and stabilities. The larger enzyme was 3-fold more active (turnover number: 2,230 +/- 97 s(-1)). Pre-treatment of bentonite with H(2)O(2) did not affect adsorption characteristics of dextranase. Enzyme to bentonite ratios above 0.5:1 (w/w) resulted in a high conservation of activity upon adsorption. Furthermore, dextranase could be used in co-immobilizates for the direct conversion of sucrose into isomalto-oligosaccharides (e.g. isomaltose). Yields of co-immobilizates were 2-20 times that of basic immobilizates, which consist of dextransucrase without dextranase.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Erhardt FA,Stammen S,Jördening HJdoi
10.1007/s10529-008-9659-8subject
Has Abstractpub_date
2008-06-01 00:00:00pages
1069-73issue
6eissn
0141-5492issn
1573-6776journal_volume
30pub_type
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