Abstract:
:Dodecanol (1% v/v) and dodecanoic acid (1% w/v) inhibited growth of Yarrowia lipolytica in complex media supplemented with glucose but dodecanedioic acid (1% w/v) was not toxic. Dodecanol-tolerant strains were prepared from the wild type strain H222 as well as the acyl-CoA oxidase deleted (deltaPOX2, POX3, POX5) strain MTLY35. These strains grew in rich media containing up to 10% (v/v) dodecanol. Dodecanol-tolerant strains remained dodecanol tolerant after they had been cultured in rich media without dodecanol. No significant amount of dodecanedioic acid was accumulated by the dodecanol-tolerant strains when grown on glucose in the presence of dodecanol.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Smit MS,Mokgoro MM,Setati E,Nicaud JMdoi
10.1023/b:bile.0000025890.97067.98keywords:
subject
Has Abstractpub_date
2004-05-01 00:00:00pages
849-54issue
10eissn
0141-5492issn
1573-6776pii
5274406journal_volume
26pub_type
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