Abstract:
:In vitro biocatalysis with cytochrome P450 BM-3 was investigated aiming for the substitution of the expensive natural cofactor NADPH by electrochemistry. The monooxygenase was immobilized on electrodes by entrapment in polypyrrole as a conductive polymer for electrochemically wiring the enzyme. Electropolymerization of pyrrole proved to be a useful means of immobilising an active cytochrome P450 BM-3 mutein on platinum and glassy carbon electrodes without denaturation. Repeatedly sweeping the electric potential between -600 and +600 mV versus Ag/AgCl led to enzymatically-catalysed product formation while in the absence of the enzyme no product formed under otherwise identical conditions.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Holtmann D,Mangold KM,Schrader Jdoi
10.1007/s10529-009-9925-4subject
Has Abstractpub_date
2009-05-01 00:00:00pages
765-70issue
5eissn
0141-5492issn
1573-6776journal_volume
31pub_type
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pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2003-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2008-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2015-11-01 00:00:00