Abstract:
:The gene for beta-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis strain DB104 for efficient and economical mass-production of the enzyme. We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth. The efficiency of production was approximately 130-fold higher than that in E. coli. The enzyme produced neoagarohexaose, neoagarotetraose and neoagarobiose from agar. Neoagarooligosaccharides produced by the enzyme had a whitening effect and inhibited tyrosinase activity in the murine melanoma cell line, B16F10. Neoagarooligosaccharides were not cytotoxic to B16F10 or normal cells. beta-Agarase could therefore be a good whitening, cosmetic additive.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Lee DG,Jang MK,Lee OH,Kim NY,Ju SA,Lee SHdoi
10.1007/s10529-008-9634-4subject
Has Abstractpub_date
2008-05-01 00:00:00pages
911-8issue
5eissn
0141-5492issn
1573-6776journal_volume
30pub_type
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更新日期:2004-04-01 00:00:00
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更新日期:2004-03-01 00:00:00
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更新日期:2006-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2010-11-01 00:00:00
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更新日期:2007-09-01 00:00:00