Micropropagation of Ajuga species: a mini review.

abstract：:Ethanol, added as a de-emulsifier to separate oil and biocatalyst (or bacterial cells) from a three-phase (oil/biocatalyst/aqueous phase) emulsion, formed in diesel biodesulfurization employing Gordonia nitida, improved oil recovery by centrifugation from about 50% in its absence to almost 100% at 3% (v/v). The biocat...

journal_title：Biotechnology letters

authors： Choi OK,Choi KS,Ryu HW,Chang YK

更新日期：2003-01-01 00:00:00

abstract：:A new ribonuclease (RNase) with tobacco mosaic virus inhibition was isolated and purified from Bacillus cereus ZH14 through ammonium sulfate precipitation, ultrafiltration, ion-exchange chromatography of DEAE-Sephadex A-50 column, and gel chromatography of Sephacryl S-200HR column. The enzyme was purified approximatel...

journal_title：Biotechnology letters

authors： Zhou WW,Niu TG

更新日期：2009-01-01 00:00:00

abstract：:The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix o...

journal_title：Biotechnology letters

authors： Camperi SA,Iannucci NB,Albanesi GJ,Oggero Eberhardt M,Etcheverrigaray M,Messeguer A,Albericio F,Cascone O

更新日期：2003-09-01 00:00:00

abstract：:Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFa monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth (fro...

journal_title：Biotechnology letters

authors： Foster LJ,Catzel D,Atwa S,Zarka M,Mahler SM

更新日期：2003-08-01 00:00:00

abstract：OBJECTIVE:Microalgae gained interest for potential use as biodiesel producers, since they synthesize and accumulate significant quantities of lipids. The aim of this work was to isolate indigenous microalgae strains from Greek habitats, study their physicochemical growth conditions and finally select the best ones with...

journal_title：Biotechnology letters

authors： Savvides AL,Moisi K,Katsifas EA,Karagouni AD,Hatzinikolaou DG

更新日期：2019-05-01 00:00:00

abstract：OBJECTIVE:The dye-decolorizing peroxidase from Bacillus amyloliquefaciens, BaDyP, was identified to be an efficient catalyst for the degradation of phenolic β-ether lignin model dimer guaiacylglycerol-β-guaiacyl ether (GGE) and dyes. RESULTS:Efeb gene encoding BaDyP from B. amyloliquefaciens MN-13 consisted of 1257 bp...

journal_title：Biotechnology letters

authors： Yang J,Gao T,Zhang Y,Wang S,Li H,Li S,Wang S

更新日期：2019-09-01 00:00:00

abstract：:The shape of the hydrophobic tunnel leading to the active site of Penicillium expansum lipase (PEL) was redesigned by single-point mutations, in order to better understand enzyme enantioselectivity towards naproxen. A variant with a valine-to-glycine substitution at residue 237 exhibited almost no enantioselectivity (...

journal_title：Biotechnology letters

authors： Tang L,Su M,Chi L,Zhang J,Zhang H,Zhu L

更新日期：2014-03-01 00:00:00

abstract：:A gene encoding a gamma-butyrolactone autoregulator receptor was cloned in to E. coli from Streptomyces ambofaciens producing spiramycin, a macrolide antibiotic used in both veterinary medicine and human medicine. A 714-bp intact receptor gene (saaR) was obtained by PCR and genomic Southern hybridization with the 100-...

journal_title：Biotechnology letters

authors： Choi SU,Kim MK,Ha HS,Hwang YI

更新日期：2008-05-01 00:00:00

abstract：:Brevibacterium linens forms hydrolytic enzymes which can be used to accelerate the ripening of cheese without causing bitterness. B. linens ATCC 9172 was grown to a high cell density (50 g dry wt l-1 after 60 h) in a mineral medium containing lactic acid, soy-peptone and ammonium sulphate by applying a continuous feed...

journal_title：Biotechnology letters

authors： Adamitsch BF,Karner F,Hampel WA

更新日期：2003-05-01 00:00:00

abstract：:Microcycle conidiation is a survival mechanism of fungi encountering unfavorable conditions. In this phenomenon, asexual spores germinate secondary spores directly without formation of mycelium. As Penicillium camemberti conidia have the ability to produce conidiophores after germination in liquid culture induced by a...

journal_title：Biotechnology letters

authors： Boualem K,Gervais P,Cavin JF,Waché Y

更新日期：2014-11-01 00:00:00

abstract：:Potato (Solanum tuberosum) hairy root cultures, established by infecting potato tuber discs with Agrobacterium rhizogenes, were used as a model system for the production of antimicrobial sesquiterpenes and lipoxygenase (LOX) metabolites. Of the four sesquiterpene phytoalexins (rishitin, lubimin, phytuberin and phytube...

journal_title：Biotechnology letters

authors： Komaraiah P,Reddy GV,Reddy PS,Raghavendra AS,Ramakrishna SV,Reddanna P

更新日期：2003-04-01 00:00:00

abstract：:The shuttle vector pGYC4α (6,157 bp) was constructed based on the sigma-replicon plasmid pYC2 from Lactobacillus sakei BM5 isolated from kimchi. The vector contained inserts of the ColE1 replicon, α-amylase gene from Bacillus licheniformis containing its own signal peptide, and lactococcal promoter P32. Transformation...

journal_title：Biotechnology letters

authors： Yang EJ,Chang HC

更新日期：2011-03-01 00:00:00

abstract：:A strain of Pseudomonas stutzeri ZWLR2-1 was isolated from soil contaminated with chloronitrobenzenes and identified by 16S rDNA sequencing. This bacterium released chloride and nitrite into the medium when grown on 0.5 mM 2-chloronitrobenzene. PCR amplification and DNA sequencing revealed a DNA fragment encoding a po...

journal_title：Biotechnology letters

authors： Liu H,Wang SJ,Zhou NY

更新日期：2005-02-01 00:00:00

abstract：:Growth of Ruta graveolens shoots was induced when Bacillus sp. cell lysates were added to the culture medium. Elicitation of coumarin by this lysate was also very effective; the concentrations of isopimpinelin, xanthotoxin and bergapten increased to 610, 2120 and 1460 microg g(-1) dry wt, respectively. It also had a s...

journal_title：Biotechnology letters

authors： Orlita A,Sidwa-Gorycka M,Malinski E,Czerwicka M,Kumirska J,Golebiowski M,Lojkowska E,Stepnowski P

更新日期：2008-03-01 00:00:00

abstract：:Rhodococcus sp. CGMCC 4911 transformed 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives into corresponding diols. Ethylene sulfate, glycol sulfide, 1,3-PDS, and 1,2-propanediol cyclic sulfate were effectively hydrolyzed with growing cells. (R)-1,2-Propanediol (>99 % e.e.) was obtained at 44 % yield with gr...

journal_title：Biotechnology letters

authors： He YC,Tao ZC,Zhang DP,Yang ZX,Gao S,Ma CL

更新日期：2015-01-01 00:00:00

abstract：:Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 degrees C, and Lac II was at pH 4.2 and 50 degrees C...

journal_title：Biotechnology letters

authors： Garcia TA,Santiago MF,Ulhoa CJ

更新日期：2006-05-01 00:00:00

abstract：:Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...

journal_title：Biotechnology letters

authors： Tani Y,Amaishi Y,Funatsu T,Ito M,Itonori S,Hata Y,Ashida H,Yamamoto K

更新日期：2014-12-01 00:00:00

abstract：:Microbial consortia obtained from soil samples of gasoline-polluted sites were individually enriched with pentane, hexane, isooctane and toluene. Cometabolism with methyl tert-butyl ether, (MTBE), gave maximum degradation rates of 49, 12, 32 and 0 mg g(-1)protein h(-1), respectively. MTBE was fully degraded even when ...

journal_title：Biotechnology letters

authors： Morales M,Velázquez E,Jan J,Revah S,González U,Razo-Flores E

更新日期：2004-02-01 00:00:00

abstract：OBJECTIVES:Developing an effective HIV vaccine that stimulates the humoral and cellular immune responses is still challenging because of the diversity of HIV-1 virus, polymorphism of human HLA and lack of a suitable delivery system. RESULTS:Using bioinformatics tools, we designed a DNA construct encoding multiple epit...

journal_title：Biotechnology letters

authors： Davoodi S,Bolhassani A,Sadat SM,Irani S

更新日期：2019-11-01 00:00:00

abstract：:Marinomonas sp. NJ522, isolated from Antarctic sea ice, produces a cold-active iron superoxide dismutase (SOD; EC 1.15.1.1). The purified SOD was dimeric and had an approx. Mr of 48 kDa. Highest activity was detected from pH 8 to 10 and at 40 degrees C (assayed over 10 min). Activity at 0 degree C was nearly 35% of th...

journal_title：Biotechnology letters

authors： Zheng Z,Jiang YH,Miao JL,Wang QF,Zhang BT,Li GY

更新日期：2006-01-01 00:00:00

abstract：:Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...

journal_title：Biotechnology letters

authors： Jin X,Rao M,Wei W,Ge M,Liu J,Chen D,Liang Y

更新日期：2012-12-01 00:00:00

abstract：:Dodecanol (1% v/v) and dodecanoic acid (1% w/v) inhibited growth of Yarrowia lipolytica in complex media supplemented with glucose but dodecanedioic acid (1% w/v) was not toxic. Dodecanol-tolerant strains were prepared from the wild type strain H222 as well as the acyl-CoA oxidase deleted (deltaPOX2, POX3, POX5) strai...

journal_title：Biotechnology letters

authors： Smit MS,Mokgoro MM,Setati E,Nicaud JM

更新日期：2004-05-01 00:00:00

abstract：OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. ...

journal_title：Biotechnology letters

authors： Akasov R,Gileva A,Zaytseva-Zotova D,Burov S,Chevalot I,Guedon E,Markvicheva E

更新日期：2017-01-01 00:00:00

abstract：:The volumetric productivity of the beer fermentation process can be increased by using a higher pitching rate (i.e. higher inoculum size). However, the decreased yeast net growth observed in these high cell density brewery fermentations can adversely affect the physiological stability throughout subsequent yeast gener...

journal_title：Biotechnology letters

authors： Verbelen PJ,Dekoninck TM,Van Mulders SE,Saerens SM,Delvaux F,Delvaux FR

更新日期：2009-11-01 00:00:00

abstract：:A very high gravity (VHG) repeated-batch fermentation system using an industrial strain of Saccharomyces cerevisiae PE-2 (isolated from sugarcane-to-ethanol distillery in Brazil) and mimicking industrially relevant conditions (high inoculation rates and low O(2) availability) was successfully operated during fifteen c...

journal_title：Biotechnology letters

authors： Pereira FB,Gomes DG,Guimarães PM,Teixeira JA,Domingues L

更新日期：2012-01-01 00:00:00

abstract：:Human beta-amyloid (Abeta) is believed to be one of the main components of Alzheimer's disease, so reduction of Abeta is considered a key therapeutic target. Using Agrobacterium-mediated nuclear transformation, we generated transgenic tomatoes for Abeta with tandem repeats. Integration of the human Abeta gene into the...

journal_title：Biotechnology letters

authors： Youm JW,Jeon JH,Kim H,Kim YH,Ko K,Joung H,Kim H

更新日期：2008-10-01 00:00:00

abstract：:ECO-orf27 associated with the cluster of ECO-0501 (LW01) from Amycolatopsis orientalis is deduced to encode a type II thioesterase. Disruption of ECO-orf27 reduced LW01 production by 95 %. Complementation of the disrupted mutant with intact ECO-orf27 restored the production of LW01 suggesting that ECO-orf27 is crucial...

journal_title：Biotechnology letters

authors： Shen Y,Huang H,Zhu L,Luo M,Chen D

更新日期：2012-11-01 00:00:00

abstract：:The inhibition of protein-tyrosine phosphatase 1B (PTP1B) is a potential target for treatment of type 2 diabetes. Vanadium and zinc metal coordinated complexes have insulin-enhancing activities, and while vanadium compounds inhibit PTP1B, little is known on the mode of action of zinc compounds. In this study we develo...

journal_title：Biotechnology letters

authors： Seale AP,de Jesus LA,Kim SY,Choi YH,Lim HB,Hwang CS,Kim YS

更新日期：2005-02-01 00:00:00

abstract：:Recombinant Mycobacterium sp. strain MR65 carrying dszABCD genes was used for desulfurization of 10-methylbenzo[b]naphtho[2,1-d]thiophene (10-methyl BNT) in the hexadecane phase. The specific activity was 25% of that of dibenzothiophene (DBT). One of two major metabolites of 10-methyl BNT produced by strain MR65 was i...

journal_title：Biotechnology letters

authors： Watanabe K,Noda K,Maruhashi K

更新日期：2003-05-01 00:00:00

abstract：:The Rho1 protein is a GTPase that participates in cell wall biogenesis. We analyzed the transcript levels of laccase genes (lccl, lcc2, lcc3, lcc4, lcc5, and lcc9), and a xylanase gene (xyl3) in Fusarium oxysporum f. sp. lycopersici strain 4287 (wild type) and two mutant strains; rhol::hyg that lacks a functional Rho1...

journal_title：Biotechnology letters

authors： Reyes-Medina MA,Macías-Sánchez KL

更新日期：2015-03-01 00:00:00