Abstract:
:A very high gravity (VHG) repeated-batch fermentation system using an industrial strain of Saccharomyces cerevisiae PE-2 (isolated from sugarcane-to-ethanol distillery in Brazil) and mimicking industrially relevant conditions (high inoculation rates and low O(2) availability) was successfully operated during fifteen consecutive fermentation cycles, attaining ethanol at 17.1 ± 0.2% (v/v) with a batch productivity of 3.5 ± 0.04 g l(-1) h(-1). Moreover, this innovative operational strategy (biomass refreshing step) prevented critical decreases on yeast viability levels and promoted high accumulation of intracellular glycerol and trehalose, which can provide an adaptive advantage to yeast cells under harsh industrial environments. This study contributes to the improvement of VHG fermentation processes by exploring an innovative operational strategy that allows attaining very high ethanol titres without a critical decrease of the viability level thus minimizing the production costs due to energy savings during the distillation process.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Pereira FB,Gomes DG,Guimarães PM,Teixeira JA,Domingues Ldoi
10.1007/s10529-011-0735-0subject
Has Abstractpub_date
2012-01-01 00:00:00pages
45-53issue
1eissn
0141-5492issn
1573-6776journal_volume
34pub_type
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