Abstract:
:The shuttle vector pGYC4α (6,157 bp) was constructed based on the sigma-replicon plasmid pYC2 from Lactobacillus sakei BM5 isolated from kimchi. The vector contained inserts of the ColE1 replicon, α-amylase gene from Bacillus licheniformis containing its own signal peptide, and lactococcal promoter P32. Transformation and expression of a selection marker gene (α-amylase) with pGYC4α were demonstrated in Escherichia coli and several lactic acid bacteria (LAB). The highest α-amylase activity in LAB transformants was obtained in M17/0.25% glucose media with 0.5% CaCO(3). The segregational stability of the shuttle vector in LAB was 100% for more than 100 generations in the absence of antibiotic pressure. The developed vector might be useful as a genetic tool for food industries.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Yang EJ,Chang HCdoi
10.1007/s10529-010-0467-6subject
Has Abstractpub_date
2011-03-01 00:00:00pages
599-605issue
3eissn
0141-5492issn
1573-6776journal_volume
33pub_type
杂志文章abstract:OBJECTIVE:Myelin oligodendrocyte glycoprotein (MOG) is one of the major autoantigens in multiple sclerosis (MS), therefore selective depletion of autoreactive lymphocytes exposing MOG-specific B cell receptors (BCRs) would be beneficial in terms of MS treatment. RESULTS:Using E. coli we generated an efficient protocol...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2092-5
更新日期:2016-07-01 00:00:00
abstract::Microbial solar biofuels offer great promise for future sustainable food, fuels and chemicals but are limited by low productivities and a requirement for large land areas to harvest sunlight. A 71 % increase in combined photosynthetic activity was achieved by illuminating both Rhodobacter sphaeroides and Arthrospira (...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1021-5
更新日期:2012-12-01 00:00:00
abstract::Endophytic colonization in rice was induced using rhizobia. Dehusked seeds of rice hybrid, CORH2, were used as explants for induction of calli. MS medium was modified with 2,4-D (2.5 mg l(-1)) and kinetin (0.2 mg l(-1)) for callus induction. Well-developed calli were inoculated with Azorhizobium caulinodans strains OR...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9693-6
更新日期:2008-08-01 00:00:00
abstract::Ma-pyrG was cloned from Monascus aurantiacus AS3.4384 using degenerate PCR with primers designed with an algorithm called CODEHOP, and its complete sequence was obtained by a PCR-based strategy for screening a Monascus fosmid library. Ma-pyrG encodes orotidine-5'-phosphate decarboxylase (OMPdecase), a 283-aminoacid pr...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0336-3
更新日期:2010-11-01 00:00:00
abstract::Pancreatic islet transplantation is used to treat diabetes mellitus that has minimal complications and avoids hypoglycemic shock. Conformal microencapsulation of pancreatic islets improves their function by blocking immunogenic molecules while protecting fragile islets. However, production of empty alginate capsules d...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1300-9
更新日期:2013-12-01 00:00:00
abstract::Human intestinal maltase (HMA) is an α-glucosidase that hydrolyses α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA is an important target to discover of new drugs for the treatment of type 2 diabetes. In this study, 308,307 compounds were virtually screened with HMA using Autodock 3.0.5 in a WI...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0675-8
更新日期:2011-11-01 00:00:00
abstract::The effects of a water-miscible ionic liquid, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF(4)]), on both thermodynamics and kinetic mechanism of the horseradish peroxidase (HRP)-catalyzed oxidation of guaiacol (2-methoxyphenol) by H(2)O(2) were investigated. The ionic liquid stabilized the ground state of ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9570-8
更新日期:2008-03-01 00:00:00
abstract::Deer antlers are the only mammalian appendages capable of repeated rounds of regeneration. Every year, deer antlers are shed and regrown from blastema into large branched structures of cartilage and bone. Little is known about the genes involved in antler development particularly during the later stages of ossificatio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0841-z
更新日期:2012-05-01 00:00:00
abstract::Seventeen independent transgenic rice plants with the maize anthocyanin regulatory gene Lc under control of the CaMV 35S promoter were obtained and verified by molecular identification. Ten plants showed red spikelets during early development of florets, and the degenerate florets were still red after heading. Additio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1046-9
更新日期:2013-01-01 00:00:00
abstract:PURPOSE OF WORK:The purpose of this study is to report a ι-carrageenase which degrades ι-carrageenan yielding neo-ι-carratetraose as the main product in the absence of NaCl. The gene for a new ι-carrageenase, CgiB_Ce, from Cellulophaga sp. QY3 was cloned and sequenced. It comprised an ORF of 1,386 bp encoding for a pro...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1244-0
更新日期:2013-10-01 00:00:00
abstract::A chip was developed to store DNA for medical research. The optional restriction site fixed on the chip can randomly ligate with whole human genomic DNA treated by the corresponding restriction enzyme. PCR can then use the chip as template DNA. Moreover, a chip fixing two restriction sites (e.g. EcoRI and HindIII) sho...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022626627231
更新日期:2003-03-01 00:00:00
abstract::Microbes employ cell membranes for reducing exogenous stresses. Polyamino acid display on microbial cell surfaces and their effects on microbial chemical stress tolerance were examined. Growth analysis revealed that displays of polyarginine, polyaspartate and polytryptophan substantially enhanced tolerance of Escheric...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1689-9
更新日期:2015-02-01 00:00:00
abstract::Promoters belong to the most important regulatory domains in genomic sequences. A novel cloning method is described to produce various DNA fragments with the average size about 100 bp, which differ by 1 bp, for screening of the osmotic pressure-inducible promoters from the genome of Escherichia coli. An osmotic pressu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9583-3
更新日期:2008-04-01 00:00:00
abstract::A carboxylesterase gene from thermophilic bacterium, Alicyclobacillus tengchongensis, was cloned and expressed in Escherichia coli BL21 (DE3). The gene coded for a 513 amino acid protein with a calculated molecular mass of 57.82 kDa. The deduced amino acid sequence had structural features highly conserved among serine...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1195-5
更新日期:2013-08-01 00:00:00
abstract::Neural cortical cells, isolated from prenatal rat cerebra, were grown on surface-modified poly(lactic-co-glycolic acid, 65:35) (PLGA) films coated with poly-D: -lysine (PDL) with either laminin (LN), fibronectin (FN) or collagen (CN). Immunocytochemistry showed that the isolated cells were highly immunopositive for bo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-6585-2
更新日期:2005-01-01 00:00:00
abstract::Sodium butyrate is commonly used in mammalian cell cultures to increase the productivity of recombinant proteins. A Chinese hamster ovary (CHO) cell line producing recombinant human erythropoietin (rhEPO) was cultured in commercial medium. Addition of 0.5 mM butyrate inhibited the over-growth of the cells after the me...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1442-9
更新日期:2014-05-01 00:00:00
abstract::Tandem affinity purification (TAP) is a methodology for the isolation of protein complexes from endogenous sources. It involves incorporation of a dual-affinity tag into the protein of interest and introduction of the construct into desired cell lines or organisms. Using the two affinity handles, the protein complex a...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0592-x
更新日期:2011-08-01 00:00:00
abstract::Two versions of an optimized gene that encodes human papilloma virus type 16 major protein L1 were designed according to the codon usage frequency of Pichia pastoris. Y16 was highly expressed in both P. pastoris and Hansenula polymorpha. M16 expression was as efficient as that of Y16 in P. pastoris, but merely detecta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1455-z
更新日期:2014-05-01 00:00:00
abstract::Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0832-0
更新日期:2012-04-01 00:00:00
abstract::A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of si...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1721-0
更新日期:2015-03-01 00:00:00
abstract::Ramoplanin is a lipopeptide antibiotic active against multi-drug-resistant, Gram-positive pathogens. Structurally, it contains a di-mannose moiety attached to the peptide core at Hpg(11). The biosynthetic gene cluster of ramoplanin has already been reported and the assembly of the depsipeptide has been elucidated but ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1233-3
更新日期:2013-09-01 00:00:00
abstract::The conventional method of transketolase (TKT) activity assay uses ribose 5-phosphate and xylulose 5-phosphate as substrates. However, a new method of TKT assay is currently required since xylulose 5-phosphate is no longer commercially available and is difficult to synthesize chemically. Although there are effective a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9616-y
更新日期:2008-05-01 00:00:00
abstract:OBJECTIVE:To clone monoamine oxidase N, that catalyses the selective oxidative deamination or deracemisation of amines into imines, in Pichia pastoris and prove the importance of choosing the proper expression system for its recombinant production. RESULTS:Monoamine oxidase, originating from Aspergillus niger and subj...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2450-y
更新日期:2018-01-01 00:00:00
abstract::An extracellular chitinase from Streptomyces halstedii AJ-7, a broad spectrum antifungal biocontrol agent, was characterized and purified. The apparent molecular weight of the purified protein was 55 kDa, Km value and Vmax of the protein for colloidal chitin were 3.2 mg ml(-1) and 118 micromol h(-1), respectively. The...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-1315-y
更新日期:2005-10-01 00:00:00
abstract::The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter....
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022498314354
更新日期:2003-03-01 00:00:00
abstract::The effects of the sequential application of methyl jasmonate (MeJa), salicylic acid (SA) and yeast extract (YE) to Argemone mexicana cell cultures were compared to either the sole application of each elicitor, or to the three-partite mixture. The highest sanguinarine accumulation occurred using the sequential treatme...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0770-x
更新日期:2012-02-01 00:00:00
abstract::A recombinant plasmid, pGMF, containing a gamma-glutamylcysteine synthetase gene (GSH-I) from Saccharomyces cerevisiae, was constructed with a copper-resistance gene as the selection marker and was introduced into S. cerevisiae YSF-31. The glutathione content of the recombinant strain was 1.5-fold (13.1 mg g dry cells...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000018261.14427.e8
更新日期:2004-03-01 00:00:00
abstract::Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) c...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-6101-3
更新日期:2005-06-01 00:00:00
abstract::A strain of Serratia sp. showed intracellular electron-transparent inclusion bodies when incubated in the presence of citrate and glycerol 2-phosphate without nitrogen source following pre-growth under carbon-limitation in continuous culture. About 1.3 mmol citrate were consumed per 450 mg biomass, giving a calculated...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9561-9
更新日期:2008-03-01 00:00:00
abstract::Cell division of the wild type strain Corynebacterium (formerly Brevibacterium) ammoniagenes ATCC 6872 which requires 1 microM Mn2+ for balanced growth was inhibited by addition of 20 mM hydroxyurea (HU) or 10 mM p-methoxyphenol (MP) to a Mn2+-supplemented fermentation medium at an appropriate time. Scanning electron ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021919404066
更新日期:2003-01-01 00:00:00