Abstract:
:A chip was developed to store DNA for medical research. The optional restriction site fixed on the chip can randomly ligate with whole human genomic DNA treated by the corresponding restriction enzyme. PCR can then use the chip as template DNA. Moreover, a chip fixing two restriction sites (e.g. EcoRI and HindIII) showed the amplification by PCR for any location of genomic DNA. Repetitive PCRs have confirmed that a DNA chip can be stored by at -4 degrees C for 2 years.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Hanaoka T,Takai O,Takahashi K,Tsugane Sdoi
10.1023/a:1022626627231keywords:
subject
Has Abstractpub_date
2003-03-01 00:00:00pages
509-12issue
6eissn
0141-5492issn
1573-6776journal_volume
25pub_type
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doi:10.1007/s10529-017-2338-x
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更新日期:2012-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-016-2115-2
更新日期:2016-08-01 00:00:00
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更新日期:2015-08-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2012-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-020-03040-7
更新日期:2021-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1802-8
更新日期:2015-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0996-2
更新日期:2012-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5794-7
更新日期:2005-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025049825720
更新日期:2003-09-01 00:00:00
abstract::5-Aminolevulinic acid (ALA) is an intermediate in the biosynthesis of tetrapyrroles. Its current production is expensive. We have developed a low-cost medium for Propionibacterium acidipropionici to produce extracellular ALA. When grown at 35 °C on a medium containing 3 % (w/v) food-grade sodium lactate supplemented w...
journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2012-09-01 00:00:00