Abstract:
:miR-21 is highly expressed in a variety of cancers, suggesting that it might play a role in the process of oncogenesis, as supported by it directly causing pre-B cell lymphomas in transgenic mice. Rm21LG transgenic mice for the conditional co-expression of miR-21 and luciferase (Luc) mediated by Cre/lox P system were generated. The homozygous Rm21LG transgenic mice were visually and readily characterized immediately after birth by whole-body fluorescence imaging. More importantly, miR-21 and Luc were successfully activated in the liver of Rm21LG/Alb-Cre double-transgenic mice, demonstrating that Rm21LG conditional transgenic system could work in a Cre-dependent manner. The combined use of this conditional miR-21 transgenic mouse line, various cell/tissue-specific Cre mouse lines and bioluminescence imaging will be a valuable tool in vivo to uncover the functions of miR-21 as oncomiR in initiating tumors.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Du T,Jia J,Lin X,Xie R,Li J,Xiao D,Xu Kdoi
10.1007/s10529-013-1327-ysubject
Has Abstractpub_date
2014-01-01 00:00:00pages
9-20issue
1eissn
0141-5492issn
1573-6776journal_volume
36pub_type
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