Abstract:
OBJECTIVES:To prepare recombinant tPep-(vascular endothelial growth factor) VEGF-B and assess its biological activity. RESULTS:This new VEGF fusion protein was constructed using a targeting peptide and prepared using E.coli. The tPep-VEGF-B was refolded from inclusion bodies and purified using affinity chromatography. Its bioactivity was determined in vitro using proliferation assay and wounding healing assay, and in vivo in zebrafish. By using the optimized downstream process, recombinant tPep-VEGF-B can be obtained with a purity of >90 % and a yield of 80 mg protein/l culture medium. The refolded protein is highly effective in promoting cell migration in vitro and in enhancing angiogenesis in vivo. CONCLUSION:We have constructed a new VEGF fusion protein with potential therapeutic application in treating metabolic diseases.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Zhang Y,Tong Y,Gao M,Luo C,Song X,Gao X,Yao Wdoi
10.1007/s10529-016-2081-8subject
Has Abstractpub_date
2016-07-01 00:00:00pages
1115-20issue
7eissn
0141-5492issn
1573-6776pii
10.1007/s10529-016-2081-8journal_volume
38pub_type
杂志文章abstract::The ability of Aspergillus strains to utilize thin stillage to produce malic acid was compared. The highest malic acid was produced by Aspergillus niger ATCC 9142 at 17 g l(-1). Biomass production from thin stillage was similar with all strains but ATCC 10577 was the highest at 19 g l(-1). The highest malic acid yield...
journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
pub_type: 杂志文章
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doi:10.1007/s10529-012-0924-5
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Biotechnology letters
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更新日期:2018-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1007/s10529-005-6181-0
更新日期:2006-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-6721-7
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pub_type: 杂志文章
doi:10.1007/s10529-013-1442-9
更新日期:2014-05-01 00:00:00
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更新日期:2010-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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更新日期:2006-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9215-3
更新日期:2007-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0857-z
更新日期:2012-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1154-1
更新日期:2013-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2006-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2136-x
更新日期:2016-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000036604.20963.da
更新日期:2004-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-010-0347-0
更新日期:2010-11-01 00:00:00