Abstract:
:Three chloroplast genes, chlL, chlN and chlB, encoding the light-independent protochlorophyllide oxidoreductase (LIPOR) in Chlorella protothecoides CS-41 growing either photoautotrophically, mixotrophically or heterotrophically, were all transcribed constitutively independent of illumination and presence of glucose. Steady-state amounts of all three transcripts in the light-grown cells were, however, approximately two- to three-fold greater than those in the dark-grown cells. In addition, Western blotting demonstrated that approximately the same amount of protein was present in cultures grown mixtrophically or heterotrophically both containing glucose. However, much less protein was in photoautotrophic cells. These results suggest that LIPOR activity depends on post-transcriptional and post-translational regulation. Moreover, the fact that LIPOR accumulates in the light-grown cells indicates that LIPOR, which was thought to work only in darkness, may partially account for the protochlorophyllide photoreduction in light. Therefore, LIPOR switches on both in light and in darkness.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Shi C,Shi Xdoi
10.1007/s10529-005-5528-xkeywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
261-5issue
4eissn
0141-5492issn
1573-6776journal_volume
28pub_type
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