Abstract:
:The production of a low-temperature alkalophilic lipase from Acinetobacter johnsonii was improved using genome shuffling. The starting populations, obtained by UV irradiation and diethyl sulfate mutagenesis, were subjected to recursive protoplast fusion. The optimal conditions for protoplast formation and regeneration were 0.15 mg lysozyme/ml for 45 min at 37°C. The protoplasts were inactivated under UV for 20 min or heated at 60°C for 60 min and a fusant probability of ~98% was observed. The positive colonies were created by fusing the inactivated protoplasts. After two rounds of genome shuffling, one strain, F22, with a lipase activity of 7 U/ml was obtained.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Wang H,Zhang J,Wang X,Qi W,Dai Ydoi
10.1007/s10529-011-0749-7subject
Has Abstractpub_date
2012-01-01 00:00:00pages
145-51issue
1eissn
0141-5492issn
1573-6776journal_volume
34pub_type
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