Abstract:
:The combined analysis of peptide mass fingerprinting and 2-DE/MS using the induced and selected protein spots following growth of Pseudomonas sp. DU102 on benzoate or p-hydroxybenzoate revealed not only alpha- and beta-subunits of protocatechuate 3,4-dioxygenase but also catechol 1,2-dioxygenase responsible for ortho-pathway through ring-cleavage of aromatic compounds. Toluate 1,2-dioxygenase and p-hydroxybenzoate hydroxylase were also identified. Purification of intradiol dioxygenases such as catechol 1,2-dioxygenase and protocatechuate 3,4-dioxygenase from the benzoate or p-hydroxybenzoate culture makes it possible to trace the biodegradation pathway of strain DU102 for monocyclic aromatic hydrocarbons. Interestingly, vanillin-induced protocatechuate 3,4-dioxygenase was identical in amino acid sequences with protocatechuate 3,4-dioxygenase from p-hydroxybenzoate.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Kim SI,Park SH,Kim JW,Leem SH,Shin DJ,Kim SH,Lee DH,Kahng HYdoi
10.1007/s10529-007-9443-1subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1475-81issue
10eissn
0141-5492issn
1573-6776journal_volume
29pub_type
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