Abstract:
:Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 degrees C on Luria agar containing HgCl(2). Sequence analysis of 16S rRNA genes of two isolates showed the closest matches to be with Bacillus pallidus and Ureibacillus thermosphaericus. Minimum inhibitory concentration (MIC) values for HgCl(2) were 80 microg/ml and 30 microg/ml for these isolates, respectively, compared to 10 microg/ml for B. pallidus H12 DSM3670, a mercury-sensitive control. The best-characterised mercury-resistant Bacillus strain, B. cereus RC607, had an MIC of 60 microg/ml. The new isolates had negligible mercuric reductase activity but removed Hg from the medium by the formation of a black precipitate, identified as HgS by X-ray powder diffraction analysis. No volatile H(2)S was detected in the headspace of cultures in the absence or presence of Hg(2+), and it is suggested that a new mechanism of Hg tolerance, based on the production of non-volatile thiol species, may have potential for decontamination of solutions containing Hg(2+) without production of toxic volatile H(2)S.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Glendinning KJ,Macaskie LE,Brown NLdoi
10.1007/s10529-005-2723-8keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
1657-62issue
21eissn
0141-5492issn
1573-6776journal_volume
27pub_type
杂志文章abstract::Classical purification of the glycoprotein equine chorionic gonadotropin (eCG) from serum includes pH fractionation with metaphosphoric acid, two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. A simplified process requiring only 1/3 of the solvent and improving the yield from 53 ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0512-5
更新日期:2011-05-01 00:00:00
abstract::Biosurfactants could potentially outperform traditional surfactants in many applications whilst being more sustainable to source, manufacture, use and dispose of. However, currently available fermentation production methods are too inefficient to manufacture biosurfactants for these high volume markets. Foaming in an ...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0782-6
更新日期:2012-02-01 00:00:00
abstract::The profiles of carotenoids and production of β-carotene by six eustigmatophytes, Eustigmatos magnus, Eustigmatos polyphem, Eustigmatos vischeri, Vischeria helvetica, Vischeria punctata and Vischeria stellata, grown in a bubble column photobioreactor were measured. All eustigmatophytes contained β-carotene, violaxanth...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0996-2
更新日期:2012-11-01 00:00:00
abstract::Seeds play essential roles in plant life cycle and germination is a complex process which is associated with different phases of water imbibition. Upon imbibition, seeds begin utilization of storage substances coupled with metabolic activity and biosynthesis of new proteins. Regeneration of organelles and emergence of...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-017-2393-3
更新日期:2017-10-01 00:00:00
abstract::A propanol-rinsed enzyme preparations (PREP) of papain showed an activity of 59 nmol min(-1) (mg powder)(-1) in tert-butanol at the optimal water activity of 0.2. The immobilized papain was stable in aqueous media for 3 d at 4 degrees C. Solid-state buffers (bases and their HCl salts) suspended in the organic medium d...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000012891.13904.62
更新日期:2004-01-01 00:00:00
abstract::An alginate lyase gene, algA, encoding a new poly β-D-mannuronate (polyM)-specific alginate lyase AlgA, was cloned from Pseudomonas sp. E03. The recombinant AlgA with (His)6-tag, consisting of 364 amino acids (40.4 kDa),was purified using Ni-NTA Sepharose. The purified lyase had maximal activity (222 EU/mg) at pH 8 an...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1685-0
更新日期:2015-02-01 00:00:00
abstract::Chloroperoxidase from Caldariomyces fumago catalyses the oxidation of 1,2-dihydronaphthalene to (1R,2R)-(+)-dihydroxytetrahydronaphthalene in homogenous citrate buffer/ionic liquid mixtures, using t-butyl hydroperoxide as O2 donor. It tolerates up to 30 (v/v) 1,3-dimethylimidazolium methylsulfate or 1-butyl-3-methylim...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-5087-6
更新日期:2004-12-01 00:00:00
abstract::The developmental ability among embryos produced by three different techniques were examined: there were no significant differences in the developmental rate in porcine embryos produced by in vitro fertilization (IVF) and first generation of somatic cell nucleus transfer (SCNT), but the developmental rate dropped shar...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1467-8
更新日期:2014-06-01 00:00:00
abstract::The production of biodiesel by transesterification employing acid or base catalyst has been industrially accepted for its high conversion and reaction rates. Downstream processing costs and environmental problems associated with biodiesel production and byproducts recovery have led to the search for alternative produc...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-010-0275-z
更新日期:2010-08-01 00:00:00
abstract:OBJECTIVE:To improve the production of trans-10,cis-12-conjugated linoleic acid (t10,c12-CLA) from linoleic acid in recombinant Yarrowia lipolytica. RESULTS:Cells of the yeast were permeabilized by freeze/thawing. The optimal conditions for t10,c12-CLA production by the permeabilized cells were at 28 °C, pH 7, 200 rpm...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2175-3
更新日期:2016-11-01 00:00:00
abstract:OBJECTIVE:To compare methods for erythroid differentiation of K562 cells that will be promising in the treatment of beta-thalassemia by inducing γ-globin synthesis. RESULTS:Cells were treated separately with: RPMI 1640 medium without glutamine, RPMI 1640 medium without glutamine supplemented with 1 mM sodium butyrate,...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2101-8
更新日期:2016-08-01 00:00:00
abstract::16,17-Epoxysterol plays an important role in pharmaceutical steroid synthesis. To investigate the potential application of cytochrome P450 for epoxysterol synthesis, an approach to the epoxidation of 16,17-epoxysterol, based on directed evolution of cytochrome P450 BM-3, was developed. This comprised random gene mutag...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1254-y
更新日期:2013-10-01 00:00:00
abstract:OBJECTIVES:To screen the phylogenetically-nearest members of Cellulosimicrobium cellulans for the production of cellulosome-like multienzyme complexes and extracellular β-xylosidase activity against 7-xylosyltaxanes and to get corresponding molecular insights. RESULTS:Cellulosimicrobium (family Promicromonosporaceae) ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2469-0
更新日期:2018-02-01 00:00:00
abstract::The exposure of CHO DG44 cells to an osmotic shock, after DNA uptake, results in a cellular volume decrease of approx. 55%. Repetitive osmotic shocks targeted different sub-populations of cells as was demonstrated using two different fluorescent reporter genes. Also the exposure of a calcium phosphate-DNA coprecipitat...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9167-7
更新日期:2006-11-01 00:00:00
abstract::The genetic relationship among commercial cultivars of Citrus limon (lemon) was analysed by inter-simple sequence repeats (ISSR) and flow cytometry techniques. Two cultivars with a close germplasm were distinguished by screening 10 SSR primers and by measuring DNA content of prestained nuclei. ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000044920.36497.fc
更新日期:2004-08-01 00:00:00
abstract::Motif analysis of PGa4 indicated that eight consecutive guanine residues were most important for pyrroloquinoline quinine glucose dehydrogenase (PQQGDH) recognition. Because the truncated mutants minimized unexpected interactions between PGa4 and the target-binding aptamer, they will now facilitate the construction of...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0292-y
更新日期:2010-09-01 00:00:00
abstract::Dodecanol (1% v/v) and dodecanoic acid (1% w/v) inhibited growth of Yarrowia lipolytica in complex media supplemented with glucose but dodecanedioic acid (1% w/v) was not toxic. Dodecanol-tolerant strains were prepared from the wild type strain H222 as well as the acyl-CoA oxidase deleted (deltaPOX2, POX3, POX5) strai...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000025890.97067.98
更新日期:2004-05-01 00:00:00
abstract::A novel micro-bioreactor based on the oscillatory flow technology was applied to the scale-down of the biotechnological production of gamma-decalactone. A decrease up to 50% of the time required to obtain the maximum concentration of the compound was observed, when compared with other scaled-down platforms (stirred ta...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-0003-x
更新日期:2006-04-01 00:00:00
abstract::Peroxisome proliferator-activated receptors (PPARs) alpha and gamma are key regulators of lipid homeostasis and insulin resistance. In this study we show that a novel compound, 3-{4-[2-(5-methyl-2-phenyl-oxazol-4-yl)-ethoxy]-phenyl}- 2-[2-(2-nitro-phenoxy)-acetyl amino]-propionic acid (O325H), is an agonist with dual ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9013-y
更新日期:2006-06-01 00:00:00
abstract::Amberlite XAD 2 resin enhanced the asymmetric reduction of ethyl 4-chloroacetoacetate (ECA) to S-4-chloro-3-hydroxybutyric acid ethyl ester as catalyzed by Saccharomyces cerevisiae. The absorbed ECA was released slowly to the solution during the reaction so that the substrate inhibition and the spontaneous chemical hy...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1021761627090
更新日期:2003-01-01 00:00:00
abstract:PURPOSE OF WORK:Thymidine is an important precursor in antiviral drugs. We have enhanced thymidine production in E. coli by eliminating the repressors in the transcription of the gene coding for carbamoyl phosphate synthetase. The operon for carbamoyl phosphate synthetase (CarAB) in the thymidine biosynthesis regulator...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0413-7
更新日期:2011-01-01 00:00:00
abstract::Alginate production by Azotobacter vinelandii growing in chemostat cultures was evaluated under different O(2) transfer rates (OTR). As a result of modifying the culture's agitation rate from 300 to 500 rpm, the OTR increased from 9 to 15.1 mmol l(-1) h(-1) and a slight variation in the alginate production (1.7-2.2 g ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9949-9
更新日期:2009-06-01 00:00:00
abstract::To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1489-2
更新日期:2014-06-01 00:00:00
abstract::A recombinant gene coding for an antibody-targeted urokinase-type plasminogen activator was constructed for the purpose of enhancing the thrombolytic specificity of urokinase. The recombinant gene was cloned into prokaryotic expression vector pTrcHisA, and transformed into Escherichia coli strain Rosetta (DE3). Less t...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-3185-0
更新日期:2004-11-01 00:00:00
abstract::The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1140-7
更新日期:2013-05-01 00:00:00
abstract::Many enzymes used as digestive aids exhibit, at best, moderate stability when incubated under gastrointestinal conditions. A supplemental β-galactosidase administered orally to treat lactose intolerance was conjugated to 40 kDa, branched polyethylene glycol (PEG). PEGylation increased the enzyme's relative activity at...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0474-7
更新日期:2011-03-01 00:00:00
abstract:OBJECTIVE:To evaluate the therapeutic potential of human umbilical cord blood mesenchymal stem cells (hUCBMSCs) on promoting erectile function in a rat model of bilateral cavernous nerve (CN) crush injury. RESULTS:Fifty male Sprague-Dawley rats were randomly assigned to sham + PBS group (n = 10), BCNI (bilateral caver...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-015-1816-2
更新日期:2015-07-01 00:00:00
abstract::Long-term cell recycle fermentations of Candida tropicalis were performed over 14 rounds of fermentation. The average xylitol concentrations, fermentation times, volumetric productivities and product yields for 14 rounds were 105 g l(-1), 333 h, 4.4 g l(-1) h(-1) and 78%, respectively, in complex medium; and 110 g l(-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000023019.02411.54
更新日期:2004-04-01 00:00:00
abstract::In Klebsiella pneumoniae, aldehyde dehydrogenases (ALDH) convert 3-hydroxypropionaldehyde (3-HPA) into 3-hydroxypropionic acid (3-HP). Although ALDHs can increase the production of 3-HP in K. pneumoniae, the substrate specificity of ALDH homologues from other microorganisms toward 3-HPA is less documented. Here we rep...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1730-z
更新日期:2015-03-01 00:00:00
abstract::An optimal pooling system, called Accurate and Fast Target Screening, has been developed for high-throughput identifying the rare marker-free transformants. This system can identify targets between 10- and 100-fold more efficiently than analysis of individual samples. By calculating the efficiency for different propor...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9129-0
更新日期:2006-10-01 00:00:00