Inter-simple sequence repeat markers and flow cytometry for the characterization of closely related Citrus limon germplasms.

abstract：:We proposed a novel combined gene therapy of human vascular endothelial growth factor 165 gene (hVEGF165) and human bone morphogenetic protein 2 gene (hBMP2) for bone regeneration by lentivirus-mediated co-transfection of both genes into rat bone marrow-derived mesenchymal stromal cells (MSCs). Both genes were success...

journal_title：Biotechnology letters

authors： Jiang J,Fan CY,Zeng BF

更新日期：2008-02-01 00:00:00

abstract：:Salinispora arenicola CNS-205 was a first-isolated obligate marine actinomycete. A gene (sare0357), annotated as ''amino acid adenylation domain'' located on the genome of Salinispora arenicola CNS-205, was cloned and characterized. The recombinant target protein Sare0357 was expressed in E. coli. Sare0357 specificall...

journal_title：Biotechnology letters

authors： Shu X,Ma Y,Lu C,Lei M,Liu Y,Liu K,Xiong G,Xia S,Zhao Z,Luo W,Fu Q,Qi C

更新日期：2015-05-01 00:00:00

abstract：:The lipase-catalyzed reaction is useful to obtain sugar esters with chemically defined structures and will contribute to the synthesis of sugar-based compounds by a chemo-enzymatic pathway. The synthesis of sugar esters in nonaqueous media has been attempted for a quarter century. To facilitate the reactions, they hav...

journal_title：Biotechnology letters

authors： Kobayashi T

更新日期：2011-10-01 00:00:00

abstract：:Malic enzyme (ME) catalyses the oxidative decarboxylation of L-malate to pyruvate and provides NADPH for intracellular metabolism, such as fatty acid synthesis. Here, the mitochondrial ME (mME) gene from Mortierella alpina was homologously over-expressed. Compared with controls, fungal arachidonic acid (ARA; 20:4 n-6)...

journal_title：Biotechnology letters

authors： Hao G,Chen H,Du K,Huang X,Song Y,Gu Z,Wang L,Zhang H,Chen W,Chen YQ

更新日期：2014-09-01 00:00:00

abstract：:The recent progress in knowledge on biochemical properties and functions of phospholipases A(2) in plants paved the way for approving the suitability of these enzymes for commercial use now. The secreted phospholipases A(2), representing one type of phospholipases A(2) occurring in plants, show distinct differences in...

journal_title：Biotechnology letters

authors： Mansfeld J

更新日期：2009-09-01 00:00:00

abstract：:The maltose-binding protein (MBP), which possesses a large number of exposed hydrophobic zones, can be used as a link for the immobilization of growth factors. The amount of immobilized MBP-vascular endothelial growth factors (VEGFs) for polystyrene surface was increased with respect to increasing protein, showing 101...

journal_title：Biotechnology letters

authors： Han M,Park IS,Kim SH,Kim BS,Kim SH

更新日期：2009-11-01 00:00:00

abstract：:A novel glucose-sensing molecule was created based on galactose/glucose-binding protein (GGBP). GGBP mutants at Asp14, a residue interacting with the 4th hydroxyl group of the sugar molecule, were constructed by mutagenesis to improve the ligand specificity of GGBP. The autofluorescence-based analysis of the binding a...

journal_title：Biotechnology letters

authors： Sakaguchi-Mikami A,Taneoka A,Yamoto R,Ferri S,Sode K

更新日期：2008-08-01 00:00:00

abstract：:Four Cr(VI)-reducing bacterial strains (Ochrobactrum intermedium, CrT-2, CrT-3 and CrT-4) previously isolated from chromium-contaminated sites were inoculated on to seeds of sunflower (Helianthus annuus var SF-187), which were germinated and grown along with non-inoculated controls with chromate salts (300 microg CrCl...

journal_title：Biotechnology letters

authors： Faisal M,Hasnain S

更新日期：2005-07-01 00:00:00

abstract：:Image analysis was used to measure the effect of Tween 40 on the morphology of Trichoderma harzianum. The percentage of pellets was maximal (93%) with Tween at 0.2 ml l(-1), whereas the maximal proportion of dispersed mycelia (40%) was with Tween at 1.6 ml l(-1). The particle median diameter decreased from 2 to 0.5 mm...

journal_title：Biotechnology letters

authors： Lucatero S,Galindo E,Larralde-Corona CP

更新日期：2004-01-01 00:00:00

abstract：:Although resistance of microorganisms to Hg(II) salts has been widely investigated and resistant strains have been reported from many eubacterial genera, there are few reports of mercuric ion resistance in extremophilic microorganisms. Moderately thermophilic mercury resistant bacteria were selected by growth at 62 de...

journal_title：Biotechnology letters

authors： Glendinning KJ,Macaskie LE,Brown NL

更新日期：2005-11-01 00:00:00

abstract：:Some pest management programs employ PCR to identify cry1 genes from Bacillus thuringiensis to predict bacterial toxicity towards different insect pests. However, due to changes on the mode of action of the Cry proteins, new primers had to be designed to detect the new genes. Therefore, an 'in-silico' study of genetic...

journal_title：Biotechnology letters

authors： Davolos CC,Guidelli-Thuler AM,de Abreu IL,Sena JA,Lemos MV

更新日期：2009-12-01 00:00:00

abstract：:The conventional method of transketolase (TKT) activity assay uses ribose 5-phosphate and xylulose 5-phosphate as substrates. However, a new method of TKT assay is currently required since xylulose 5-phosphate is no longer commercially available and is difficult to synthesize chemically. Although there are effective a...

journal_title：Biotechnology letters

authors： Lee JY,Cheong DE,Kim GJ

更新日期：2008-05-01 00:00:00

abstract：:Aerobic granules for sulphide and ammonium removal were cultivated in a sequencing batch reactor, and the microbial community of the aerobic granules was investigated by denaturing gradient gel electrophoresis. The loading rate increased from 0.15 to 0.9 kg S2− m−3 d−1, and the removal efficiencies of sulphide, chemic...

journal_title：Biotechnology letters

authors： Su C,Zhu L,Zhang C,Qi X,Guo Y,Gao R

更新日期：2012-05-01 00:00:00

abstract：:Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fu...

journal_title：Biotechnology letters

authors： Rahman M,Hasan MR,Shimizu K

更新日期：2008-05-01 00:00:00

abstract：:A gene-specific, metagenomic PCR method has led to the discovery of a novel esterase subfamily consisting of five homologous members. Sequence analysis of this esterase subfamily, named the ArmEst subfamily, revealed a unique conserved pattern with a significant variable interior sequence flanked by two symmetric and ...

journal_title：Biotechnology letters

authors： Zhang A,Zhao R,Jin P,Ma L,Xiong X,Xie T,Pei X,Yu L,Yin X,Wang Q

更新日期：2013-11-01 00:00:00

abstract：:Trichodesmium sp., isolated from the Great Barrier Reef lagoon, was cultured in artificial seawater media containing a range of Fe concentration. Fe additions stimulated growth, N2 fixation, cellular chlorophyll a content, light-saturated chlorophyll a-specific gross photosynthetic capacity (Pmchl a) and the dark resp...

journal_title：Biotechnology letters

authors： Fu FX,Bell PR

更新日期：2003-04-01 00:00:00

abstract：OBJECTIVES:To develop a rapid, dual-parameter, plate-based screening process to improve production and secretion rate of glucose oxidase simultaneously in Aspergillus niger. RESULTS:A morphology engineering based on CaCO3 was implemented, where the yield of GOD by A. niger was increased by up to 50%. Analysis of extra...

journal_title：Biotechnology letters

authors： Zhu X,Sun J,Chu J

更新日期：2018-01-01 00:00:00

abstract：:Arylomycins are type I signal peptidase inhibitors and have a potential as a new type of antibiotics. They were identified from the broth of Streptomyces sp. HCCB10043. The arylomycin biosynthetic gene cluster in this strain was identical to that in S. roseosporus. Within the gene cluster, aryC, encoding a P450 enzyme...

journal_title：Biotechnology letters

authors： Jin X,Rao M,Wei W,Ge M,Liu J,Chen D,Liang Y

更新日期：2012-12-01 00:00:00

abstract：:A microtiter plate-based assay was developed to evaluate the ability of lipases to perform transesterifications when employed in different organic solvents. A 4-nitrophenol assay was carried out employing seven different lipase formulations and two fatty acid methyl esters with different chain lengths in a total of si...

journal_title：Biotechnology letters

authors： Pöhnlein M,Finkbeiner T,Syldatk C,Hausmann R

更新日期：2015-03-01 00:00:00

abstract：:Fluctuating light intensity had a more significant impact on growth of gametophytes of transgenic Laminaria japonica in a 2500 ml bubble-column bioreactor than constant light intensity. A fluctuating light intensity between 10 and 110 microE m(-2) s(-1), with a photoperiod of 14 h:10 h light:dark, was the best regime ...

journal_title：Biotechnology letters

authors： Gao J,Zhang Y,Zhang W,Wu S,Qin S,Zhang W,Yu X

更新日期：2005-09-01 00:00:00

abstract：OBJECTIVE:To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity. RESULTS:A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most ...

journal_title：Biotechnology letters

authors： Zhang D,Jia H,Wang Y,Li WM,Hou YC,Yin SW,Wang TD,He SX,Lu SY

更新日期：2015-11-01 00:00:00

abstract：:Streptomyces is an attractive host for heterologous protein secretion. To further optimize its expression capacity, better expression vectors will be helpful. Here, based on pSGL1, a high copy number plasmid present in Streptomyces globisporus C-1027, we constructed a series of novel E. coli-Streptomyces shuttle expre...

journal_title：Biotechnology letters

authors： Zhu Y,Wang L,Du Y,Wang S,Yu T,Hong B

更新日期：2011-02-01 00:00:00

abstract：:O-Acetylserine (thiol) lyase (OASTL, EC 2.5.1.47) catalyzes the final step in cysteine biosynthesis. The soybean GmOASTL4 gene was overexpressed in tobacco. Transgenic plants showed markedly increased accumulation of transcripts and higher cysteine content compared with the wild-type. Upon exposure to cadmium stress, ...

journal_title：Biotechnology letters

authors： Ning H,Zhang C,Yao Y,Yu D

更新日期：2010-04-01 00:00:00

abstract：:beta-Galactosidase isolated from Aspergillus oryzae was immobilized in lens-shaped polyvinylalcohol capsules (with activity 25 U g(-1)) giving 32% of its original activity. Immobilization did not change the pH optimum (4.5) of lactose hydrolysis. The relative enzyme activity during product inhibition testing was, in a...

journal_title：Biotechnology letters

authors： Grosová Z,Rosenberg M,Rebros M,Sipocz M,Sedlácková B

更新日期：2008-04-01 00:00:00

abstract：:Synthesis of long DNA fragments is often associated with mutations and requires multiple DNA manipulation steps. A novel DNA synthesis method, referred to as patch oligodeoxynucleotide synthesis (POS) to assembly long DNA fragments is presented here. This method involves connection of two types of oligodeoxynucleotide...

journal_title：Biotechnology letters

authors： Yang G,Wang S,Wei H,Ping J,Liu J,Xu L,Zhang W

更新日期：2012-04-01 00:00:00

abstract：:An index [kv: average isolation rate of viable protoplast (number/ml min)] was established to evaluate the optimal conditions for protoplast isolation from cultured plant cells. The optimal conditions for protoplasts isolation from Nicotiana tabacum BY2 cultured cells could be determined on the basis of the kv [31.7 x...

journal_title：Biotechnology letters

authors： Aoyagi H

更新日期：2006-10-01 00:00:00

abstract：:The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product ha...

journal_title：Biotechnology letters

authors： Lee JH,Kim YT

更新日期：2006-08-01 00:00:00

abstract：OBJECTIVES:To establish an automated high-throughput mimic perfusion scale-down model (SDM) in ambr® 15 system. RESULTS:An optimized SDM for mimic perfusion was developed in ambr® 15 system. Cell retention in ambr® 15 was realized by sedimentation and supernatant removal with a retention rate > 95%. Although the SDM c...

journal_title：Biotechnology letters

authors： Jin L,Wang ZS,Cao Y,Sun RQ,Zhou H,Cao RY

更新日期：2021-02-01 00:00:00

abstract：:Most high-affinity phosphate transporter genes (OsPTs) in rice were highly induced in roots when phosphate was depleted. OsPT1, however, was highly expressed in primary roots and leaves regardless of external phosphate concentrations. This finding was confirmed histochemically using transgenic rice plants that express...

journal_title：Biotechnology letters

authors： Seo HM,Jung Y,Song S,Kim Y,Kwon T,Kim DH,Jeung SJ,Yi YB,Yi G,Nam MH,Nam J

更新日期：2008-10-01 00:00:00

abstract：:Laccases play an important role in the biological break down of lignin and have great potential in the deconstruction of lignocellulosic feedstocks. We examined 16 laccases, both commercially prepared and crude extracts, for their ability to oxidize veratryl alcohol in the presence of various solvents and mediators. S...

journal_title：Biotechnology letters

authors： Larson TM,Anderson AM,Rich JO

更新日期：2013-02-01 00:00:00