Increased expression of OsPT1, a high-affinity phosphate transporter, enhances phosphate acquisition in rice.

abstract：OBJECTIVES:To design novel 3D in vitro co-culture models based on the RGD-peptide-induced cell self-assembly technique. RESULTS:Multicellular spheroids from M-3 murine melanoma cells and L-929 murine fibroblasts were obtained directly from monolayer culture by addition of culture medium containing cyclic RGD-peptide. ...

journal_title：Biotechnology letters

authors： Akasov R,Gileva A,Zaytseva-Zotova D,Burov S,Chevalot I,Guedon E,Markvicheva E

更新日期：2017-01-01 00:00:00

abstract：OBJECTIVE:To investigate the aerotolerance of Lactobacillus rhamnosus hsryfm 1301 and its influencing factors. RESULTS:The growth rate of L. rhamnosus hsryfm 1301 weakened noticeably when the concentration of supplemented H2O2 reached 1 mM, and only 2% of all L. rhamnosus hsryfm 1301 cells survived in MRS broth supple...

journal_title：Biotechnology letters

authors： Zhang C,Lu J,Yang D,Chen X,Huang Y,Gu R

更新日期：2018-04-01 00:00:00

abstract：OBJECTIVE:To develop a peptide probe that could be used for gastric cancer detection via binding to CD44 protein with specificity and affinity. RESULTS:A 12-mer phage peptide library was screened against immobilized CD44 protein. Bound phage counts using ELISA were performed to identify phage clones carrying the most ...

journal_title：Biotechnology letters

authors： Zhang D,Jia H,Wang Y,Li WM,Hou YC,Yin SW,Wang TD,He SX,Lu SY

更新日期：2015-11-01 00:00:00

abstract：:Human intestinal maltase (HMA) is an α-glucosidase that hydrolyses α-1,4-linkages from the non-reducing end of malto-oligosaccharides. HMA is an important target to discover of new drugs for the treatment of type 2 diabetes. In this study, 308,307 compounds were virtually screened with HMA using Autodock 3.0.5 in a WI...

journal_title：Biotechnology letters

authors： Nguyen TT,Ryu HJ,Lee SH,Hwang S,Cha J,Breton V,Kim D

更新日期：2011-11-01 00:00:00

abstract：:Amberlite XAD 2 resin enhanced the asymmetric reduction of ethyl 4-chloroacetoacetate (ECA) to S-4-chloro-3-hydroxybutyric acid ethyl ester as catalyzed by Saccharomyces cerevisiae. The absorbed ECA was released slowly to the solution during the reaction so that the substrate inhibition and the spontaneous chemical hy...

journal_title：Biotechnology letters

authors： Houng JY,Liau JS

更新日期：2003-01-01 00:00:00

abstract：OBJECTIVE:To construct effective genetic expression parts controlling transcription and translation initiation for synthetic biology and heterologous expression in Corynebacterium glutamicum. RESULT:Twelve highly expressed genes were identified from the proteomic data of C. glutamicum. Their related sequences were use...

journal_title：Biotechnology letters

authors： Zhao Z,Liu X,Zhang W,Yang Y,Dai X,Bai Z

更新日期：2016-12-01 00:00:00

abstract：:To elucidate the functional significance of heat-shock protein beta 1 (HSPB1) in androgen-mediated myogenesis of bovine cells, we conducted 'loss and gain of function of HSPB1' assays by siRNA inhibition and gene overexpression. siRNA inhibition of HSPB1 expression reduced the expression of desmin (a myogenic marker) ...

journal_title：Biotechnology letters

authors： Zhang Q,Lee HG,Kang SK,Baik M,Choi YJ

更新日期：2014-06-01 00:00:00

abstract：:Seeds play essential roles in plant life cycle and germination is a complex process which is associated with different phases of water imbibition. Upon imbibition, seeds begin utilization of storage substances coupled with metabolic activity and biosynthesis of new proteins. Regeneration of organelles and emergence of...

journal_title：Biotechnology letters

authors： Zaynab M,Kanwal S,Furqan M,Islam W,Noman A,Ali GM,Rehman N,Zafar S,Sughra K,Jahanzab M

更新日期：2017-10-01 00:00:00

abstract：:Microcycle conidiation is a survival mechanism of fungi encountering unfavorable conditions. In this phenomenon, asexual spores germinate secondary spores directly without formation of mycelium. As Penicillium camemberti conidia have the ability to produce conidiophores after germination in liquid culture induced by a...

journal_title：Biotechnology letters

authors： Boualem K,Gervais P,Cavin JF,Waché Y

更新日期：2014-11-01 00:00:00

abstract：OBJECTIVE:In this study, we aimed to maximize glutathione (GSH) production by a metabolically engineered Yarrowia lipolytica strain using a small-scale optimization approach. RESULTS:A three levels four factorial Box-Behnken Design was used to assess the effect of pH, inulin extract, yeast extract and ammonium sulfate...

journal_title：Biotechnology letters

authors： Do DTH,Fickers P,Ben Tahar I

更新日期：2021-02-01 00:00:00

abstract：:Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fu...

journal_title：Biotechnology letters

authors： Rahman M,Hasan MR,Shimizu K

更新日期：2008-05-01 00:00:00

abstract：:We report a novel and convenient method for the determination of glycoproteins, especially antibodies, using galactose oxidase (GAO) on the basis of the contents of galactosyl and N-acetylgalactosaminyl residues in carbohydrate chains of glycoproteins. GAO converts galactose residues to their corresponding aldehyde an...

journal_title：Biotechnology letters

authors： Jeon SI,Hong JW,Yoon HC

更新日期：2006-09-01 00:00:00

abstract：:In vitro synthesized mRNAs are often used as calibrants in gene expression analysis. In the case of an analytical procedure for gene expression containing a process of reverse transcription such as microarray analysis, full-length mRNAs having a 3'-poly(A)+tail are required as calibrants. However, the preparation of f...

journal_title：Biotechnology letters

authors： Matsuo T,Obana E,Yamamoto T,Kakuhata R,Yamazaki N,Kataoka M,Baba Y,Hori T,Shinohara Y

更新日期：2009-02-01 00:00:00

abstract：:Blakeslea trispora is used commercially to produce β-carotene. Isopentenyl pyrophosphate isomerase (IPI) and geranylgeranyl pyrophosphate synthase (GGPS) are key enzymes in the biosynthesis of carotenoids. The cDNAs of genes ipi and carG were cloned from the fungus and expressed in Escherichia coli. Greater GGPS activ...

journal_title：Biotechnology letters

authors： Sun J,Sun XX,Tang PW,Yuan QP

更新日期：2012-11-01 00:00:00

abstract：:An alginate lyase gene, algA, encoding a new poly β-D-mannuronate (polyM)-specific alginate lyase AlgA, was cloned from Pseudomonas sp. E03. The recombinant AlgA with (His)6-tag, consisting of 364 amino acids (40.4 kDa),was purified using Ni-NTA Sepharose. The purified lyase had maximal activity (222 EU/mg) at pH 8 an...

journal_title：Biotechnology letters

authors： Zhu BW,Huang LS,Tan HD,Qin YQ,Du YG,Yin H

更新日期：2015-02-01 00:00:00

abstract：:Biosurfactants could potentially outperform traditional surfactants in many applications whilst being more sustainable to source, manufacture, use and dispose of. However, currently available fermentation production methods are too inefficient to manufacture biosurfactants for these high volume markets. Foaming in an ...

journal_title：Biotechnology letters

authors： Winterburn JB,Martin PJ

更新日期：2012-02-01 00:00:00

abstract：:Biotechnology using molecular biology, biochemistry, biophysics, and computational approaches provides an alternative approach for classical pharmacological screening to look at ligand-receptor interactions and receptor specificity, which should support the design of selective drugs based on detailed structural princi...

journal_title：Biotechnology letters

authors： Ratnala VR

更新日期：2006-06-01 00:00:00

abstract：:A non-characterized gene, previously proposed as the D-tagatose-3-epimerase gene from Rhodobacter sphaeroides, was cloned and expressed in Escherichia coli. Its molecular mass was estimated to be 64 kDa with two identical subunits. The enzyme specificity was highest with D-fructose and decreased for other substrates i...

journal_title：Biotechnology letters

authors： Zhang L,Mu W,Jiang B,Zhang T

更新日期：2009-06-01 00:00:00

abstract：:Rhizopus arrhizus, strain DAR 36017, produced L(+)-lactic acid in a simultaneous saccharification and fermentation process using starch waste effluents. Lactic acid at 19.5-44.3 g l(-1) with a yield of 0.85-0.96 g g(-1) was produced in 40 h using 20-60 g starch l(-1). Supplementation of nitrogen source may be unnecess...

journal_title：Biotechnology letters

authors： Jin B,Huang LP,Lant P

更新日期：2003-12-01 00:00:00

abstract：OBJECTIVE:To identify and characterize a novel bacterial pyranose 2-oxidase (P2Ox) and investigate its potential use in lignin degradation applications. RESULTS:A new bacterial P2Ox (PaP2Ox) enzyme was identified in the lignocellulolytic bacterium Pantoea ananatis Sd-1. The PaP2Ox open reading frame was cloned, and th...

journal_title：Biotechnology letters

authors： Zhang K,Huang M,Ma J,Liu Z,Zeng J,Liu X,Xu T,Wang X,Liu Y,Bu Z,Zhu Y

更新日期：2018-05-01 00:00:00

abstract：:A new cDNA (Os beta fruct2) encoding an acid beta-D-fructofuranosidase from rice has been cloned, sequenced and expressed in Pichia pastoris. The full-length cDNA is 2453 base pairs long and encodes a pre-pro-protein of 682 amino acids. The cDNA fragment coding for mature enzyme was sub-cloned into vector pPICZalphaA ...

journal_title：Biotechnology letters

authors： Fu RH,Wang YL,Sung HY

更新日期：2003-03-01 00:00:00

abstract：OBJECTIVES:To investigate gene expression profiles of the thermotolerant yeast Saccharomyces cerevisiae strain KKU-VN8, a potential high-ethanol producer, in response to various stresses during high-temperature ethanol fermentation using sweet sorghum juice (SSJ) under optimal conditions. RESULTS:The maximal ethanol c...

journal_title：Biotechnology letters

authors： Techaparin A,Thanonkeo P,Klanrit P

更新日期：2017-10-01 00:00:00

abstract：:Human phospholipid scramblase 1 (hPLSCR1) scrambles plasma membrane phospholipids during cell activation, blood coagulation and apoptosis. It was over-expressed in E. coli with a histidine tag and purified from the inclusion bodies (*30 mg/l culture broth) under denaturing conditions using 8 M urea. The denatured hPLS...

journal_title：Biotechnology letters

authors： Sahu SK,Gopala Krishna A,Gummadi SN

更新日期：2008-12-01 00:00:00

abstract：:Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylami...

journal_title：Biotechnology letters

authors： Huh YS,Hong YK,Hong WH,Chang HN

更新日期：2004-10-01 00:00:00

abstract：:There is a great need for the development of biomimetic human tissue models that allow elucidation of the pathophysiological conditions involved in disease initiation and progression. Conventional two-dimensional (2D) in vitro assays and animal models have been unable to fully recapitulate the critical characteristics...

journal_title：Biotechnology letters

authors： Memic A,Navaei A,Mirani B,Cordova JAV,Aldhahri M,Dolatshahi-Pirouz A,Akbari M,Nikkhah M

更新日期：2017-09-01 00:00:00

abstract：:Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 degrees C, and Lac II was at pH 4.2 and 50 degrees C...

journal_title：Biotechnology letters

authors： Garcia TA,Santiago MF,Ulhoa CJ

更新日期：2006-05-01 00:00:00

abstract：OBJECTIVE:To examine the role of a gene encoding flavin-containing monooxygenase (cFMO) from Corynebacterium glutamicum ATCC13032 when cloned and expressed in Escherichia coli for the production of indigo pigments. RESULTS:The blue pigments produced by recombinant E. coli were identified as indigo and indirubin. The c...

journal_title：Biotechnology letters

authors： Ameria SP,Jung HS,Kim HS,Han SS,Kim HS,Lee JH

更新日期：2015-08-01 00:00:00

abstract：:Gene therapy is based on the principle of the genetic manipulation of DNA or RNA for treating and preventing human diseases. The clustered regularly interspaced short palindromic repeats/CRISPR associated nuclease9 (CRISPR/Cas9) system, derived from the acquired immune system in bacteria and archaea, has provided a ne...

journal_title：Biotechnology letters

authors： Mollanoori H,Teimourian S

更新日期：2018-06-01 00:00:00

abstract：:Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However...

journal_title：Biotechnology letters

authors： Minkner R,Park EY

更新日期：2018-04-01 00:00:00

abstract：:Ammonium is a substrate of the anaerobic ammonium oxidation (Anammox) process but it has been suggested as a substrate-inhibitor because of the action of its unionized form, free ammonia. High pH of the medium is also an important limiting factor of the Anammox bacteria. Both effects are difficult to discriminate. In ...

journal_title：Biotechnology letters

authors： Puyol D,Carvajal-Arroyo JM,Li GB,Dougless A,Fuentes-Velasco M,Sierra-Alvarez R,Field JA

更新日期：2014-10-01 00:00:00