Abstract:
:Trichodesmium sp., isolated from the Great Barrier Reef lagoon, was cultured in artificial seawater media containing a range of Fe concentration. Fe additions stimulated growth, N2 fixation, cellular chlorophyll a content, light-saturated chlorophyll a-specific gross photosynthetic capacity (Pmchl a) and the dark respiration rate (Rdchl a). Cell yields only doubled for 9 nM Fe relative to zero added Fe, whereas N2 fixation increased 11-fold considerably for 450 nM Fe. The results suggest that N2 fixation of Trichodesmium is more sensitive to Fe limitation than are the cell yields.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Fu FX,Bell PRdoi
10.1023/a:1023068232375keywords:
subject
Has Abstractpub_date
2003-04-01 00:00:00pages
645-9issue
8eissn
0141-5492issn
1573-6776journal_volume
25pub_type
杂志文章abstract::The industrial-scale production of phenylalanine ammonia-lyase (PAL) mainly uses strains of Rhodotorula. However, the PAL gene from Rhodotorula has not been cloned. Here, the full-length gene of PAL from Rhodotorula glutinis was isolated. It was 2,121 bp, encoding a polypeptide with 706 amino acids and a calculated MW...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1140-7
更新日期:2013-05-01 00:00:00
abstract::An acoustic cell settler (ACS) using ultrasound at cells of 3 MHz was used to recycle Saccharomyces cerevisiae in a fermenter. The locations of both the inlet and outlet in the acoustic cell settler, which have a relatively long distance between the transducer and reflector, were optimized. A tilted settler was design...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1022345508979
更新日期:2003-02-01 00:00:00
abstract::Methods for the preparation of carrier-free insoluble enzymes are reviewed. The technology of cross-linked enzyme aggregates has now been applied to a range of synthetically useful activities. Fusion proteins are also gaining momentum because they allow a relatively selective aggregation or even a specific self-assemb...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-009-0173-4
更新日期:2010-03-01 00:00:00
abstract::A lactate producing derivative of Escherichia coli KO11, strain SZ110, was re-engineered for ethanol production by deleting genes encoding all fermentative routes for NADH and randomly inserting a promoterless mini-Tn5 cassette (transpososome) containing the complete Zymomonas mobilis ethanol pathway (pdc, adhA, and a...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9821-3
更新日期:2008-12-01 00:00:00
abstract::The peptide, Ala-Pro-Ala-Arg (APAR), was selected from the screening of a tetrapeptide combinatorial synthetic library as the ligand for affinity purification of an anti-Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) monoclonal antibody (Mab) developed in mouse ascitis. The affinity chromatographic matrix o...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1025464918453
更新日期:2003-09-01 00:00:00
abstract::Microbes employ cell membranes for reducing exogenous stresses. Polyamino acid display on microbial cell surfaces and their effects on microbial chemical stress tolerance were examined. Growth analysis revealed that displays of polyarginine, polyaspartate and polytryptophan substantially enhanced tolerance of Escheric...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1689-9
更新日期:2015-02-01 00:00:00
abstract::A mutant ofS. chrysomallus with a D-glucose isomerase inducible by D-glucose or its catabolites was characterized. In contrast to the wild-type strain it showed a decreased catabolite repression by D-glucose of D-xylose consumption and a constitutive pentose phosphate pathway as well. A hypothesis concerning altered i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/BF01026199
更新日期:1987-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0559-y
更新日期:2011-06-01 00:00:00
abstract:OBJECTIVES:To prepare recombinant tPep-(vascular endothelial growth factor) VEGF-B and assess its biological activity. RESULTS:This new VEGF fusion protein was constructed using a targeting peptide and prepared using E.coli. The tPep-VEGF-B was refolded from inclusion bodies and purified using affinity chromatography....
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2081-8
更新日期:2016-07-01 00:00:00
abstract:OBJECTIVES:This short commentary examines the factors that led to Food and Drug Administration's approval of the first plant-derived biologic. RESULTS:In 2012, the first plant-derived protein pharmaceutical (biologic) was approved for commercial use in humans. The product, a recombinant form of human β-glucocerebrosid...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1908-z
更新日期:2015-11-01 00:00:00
abstract:OBJECTS:To explore the roles of growth factor receptor-bound protein 14 (GRB14) in colorectal cancer (CRC) and its correlation with clinicopathological characteristics and prognosis of CRC patients. RESULTS:GRB14 was localized in the cytoplasm of CRC and benign glandular epithelium cells, showing higher levels in CRC ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2077-4
更新日期:2016-06-01 00:00:00
abstract::Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with pH using tri-n-octylami...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000045656.00138.93
更新日期:2004-10-01 00:00:00
abstract::The gene for beta-agarase of an Agarivorans sp. JA-1 was expressed in Bacillus subtilis strain DB104 for efficient and economical mass-production of the enzyme. We isolated 360 mg protein with a specific activity of 201 U/mg from the culture broth. The efficiency of production was approximately 130-fold higher than th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9634-4
更新日期:2008-05-01 00:00:00
abstract::Genomics, transcriptomics, proteomics and fluxomics are powerful omics-technologies that play a major role in today's research. For each of these techniques good sample quality is crucial. Major factors contributing to the quality of a sample is the actual sampling procedure itself and the way the sample is stored dir...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1163-0
更新日期:2013-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2450-y
更新日期:2018-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-013-1195-5
更新日期:2013-08-01 00:00:00
abstract::Fusion expression is a common practice for recombinant protein production. Some fusion tags confer solubility on the target protein whereas others provide affinity handles that facilitate purification. However, the tag usually needs to be removed from the final product, which involves using expensive proteases or haza...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-011-0533-8
更新日期:2011-05-01 00:00:00
abstract::3,4-Dihydroxybenzoate decarboxylase in Enterobacter cloacae P241 was induced by adding 3,4-dihydroxybenzoic acid, 3-hydroxybenzoic acid, 3,4,5-trihydroxybenzoic acid or 4-acetamidobenzoic acid to the culture medium. After stabilizing the enzyme activity by adding 5 mM dithiothreitol and 20 mM Na(2)S(2)O(3) to a cell-f...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0210-3
更新日期:2010-05-01 00:00:00
abstract:OBJECTIVE:To evaluate the quantity of Spirulina cultured in seawater, salt-tolerant strains were screened out and their growth and antioxidant accumulation were studied in different salt concentrations RESULTS:Salt tolerance of five Spirulina strains were investigated with modified Zarrouk medium (with 200-800 mM NaCl...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2087-2
更新日期:2016-07-01 00:00:00
abstract::Tributyl phosphate (TBP) is widely used in nuclear fuel processing and other waste generating chemical industries. Although TBP is bacteriostatic, some microbes are resistant to it and may degrade it. Under dark aerobiosis, purple non-sulfur photosynthetic bacteria degraded up to 0.6 mM TBP, initially present at 2 mM,...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2882-7
更新日期:2005-04-01 00:00:00
abstract::The inhibition of protein-tyrosine phosphatase 1B (PTP1B) is a potential target for treatment of type 2 diabetes. Vanadium and zinc metal coordinated complexes have insulin-enhancing activities, and while vanadium compounds inhibit PTP1B, little is known on the mode of action of zinc compounds. In this study we develo...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-7855-8
更新日期:2005-02-01 00:00:00
abstract:OBJECTIVES:To develop a rapid, dual-parameter, plate-based screening process to improve production and secretion rate of glucose oxidase simultaneously in Aspergillus niger. RESULTS:A morphology engineering based on CaCO3 was implemented, where the yield of GOD by A. niger was increased by up to 50%. Analysis of extra...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2442-y
更新日期:2018-01-01 00:00:00
abstract::The astaxanthin biosynthesis gene cluster in Paracoccus haeundaensis consists of six genes: crtW, crtZ, crtY, crtI, crtB, and crtE contain 726, 486, 1158, 1503, 912, and 879 base pairs, respectively. Individual carotenoid biosynthesis genes of P. haeundaensis have now been expressed in E. coli and each gene product ha...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9072-0
更新日期:2006-08-01 00:00:00
abstract::The addresses given for the authors of this paper were incorrect. The correct assignments of the authors are given in this erratum. ...
journal_title:Biotechnology letters
pub_type: 杂志文章,已发布勘误
doi:10.1007/s10529-017-2496-x
更新日期:2018-01-01 00:00:00
abstract::The exploded bast, branch and stem of cotton stalk were degraded by alkalophilic Bacillus NT-19, with weight losses of 24%, 20% and 14%, respectively, after 14 d. Compared with a white-rot fungus (Phanerochaete chrysosporium), Bacillus NT- 19 preferentially degraded the non-cellulose components of cotton stem. The rel...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1026083827968
更新日期:2003-10-01 00:00:00
abstract:PURPOSE OF WORK:Thymidine is an important precursor in antiviral drugs. We have enhanced thymidine production in E. coli by eliminating the repressors in the transcription of the gene coding for carbamoyl phosphate synthetase. The operon for carbamoyl phosphate synthetase (CarAB) in the thymidine biosynthesis regulator...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0413-7
更新日期:2011-01-01 00:00:00
abstract::Deer antlers are the only mammalian appendages capable of repeated rounds of regeneration. Every year, deer antlers are shed and regrown from blastema into large branched structures of cartilage and bone. Little is known about the genes involved in antler development particularly during the later stages of ossificatio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0841-z
更新日期:2012-05-01 00:00:00
abstract::Baker's yeast suspensions were incubated at different pressures (from 1 bar to 6 bar) and different gases [air, O(2) and a mixture of 8% (v/v) CO(2), 21% O(2) and N(2)]. Raising the air pressure from 1 bar to 6 bar stimulated cell growth but had no effect on leavening ability or viability of the cells. A 50% reduction...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/B:BILE.0000036604.20963.da
更新日期:2004-08-01 00:00:00
abstract::Salt stress is considered one of the main abiotic factors to limit crop growth and productivity by affecting morpho-physiological and biochemical processes. Genetically, a number of salt tolerant Brassica varieties have been developed and introduced, but breeding of such varieties is time consuming. Therefore, current...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-018-2570-z
更新日期:2018-08-01 00:00:00
abstract::Theabrownins (TB) are water-soluble phenolic compounds associated with the various health benefits of Pu-erh tea, a post-fermented Chinese dark tea. This work reports on the production of theabrownins from infusions of sun-dried green tea leaves using a pure culture of Aspergillus fumigatus isolated from a solid-state...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1632-0
更新日期:2014-12-01 00:00:00