Abstract:
:Fusion expression is a common practice for recombinant protein production. Some fusion tags confer solubility on the target protein whereas others provide affinity handles that facilitate purification. However, the tag usually needs to be removed from the final product, which involves using expensive proteases or hazardous chemicals and requires additional chromatography steps. Self-cleaving tags are a special group of fusion tags that possess inducible proteolytic activity. Combined with appropriate affinity tags, they enable fusion purification, cleavage and target separation to be achieved in a single step, which saves time, labor and cost. This paper reviews currently available self-cleaving fusion tags for recombinant protein production. For each system, an introduction of its key characteristics and a brief discussion of its advantages and disadvantages is given.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Li Ydoi
10.1007/s10529-011-0533-8subject
Has Abstractpub_date
2011-05-01 00:00:00pages
869-81issue
5eissn
0141-5492issn
1573-6776journal_volume
33pub_type
杂志文章,评审abstract::A non-characterized gene, previously proposed as the D-tagatose-3-epimerase gene from Rhodobacter sphaeroides, was cloned and expressed in Escherichia coli. Its molecular mass was estimated to be 64 kDa with two identical subunits. The enzyme specificity was highest with D-fructose and decreased for other substrates i...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-009-9942-3
更新日期:2009-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2218-9
更新日期:2017-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-0985-5
更新日期:2012-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1576-4
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-004-7855-8
更新日期:2005-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-3631-7
更新日期:2005-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-2736-3
更新日期:2005-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2009-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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更新日期:2012-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1078-1
更新日期:2013-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9083-x
更新日期:2006-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2394-2
更新日期:2017-12-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-5718-6
更新日期:2006-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-005-1315-y
更新日期:2005-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0770-x
更新日期:2012-02-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
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更新日期:2010-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9757-7
更新日期:2008-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1010-8
更新日期:2012-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1019-z
更新日期:2012-11-01 00:00:00
abstract::We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H(+) transporter) and TF(0)F(1)-ATP synthase (a thermo-stable ATP synthase). These vesicles showed light-dependent ATP synthesis. Furthermore, coupling the ATP photosynthetic v...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0544-5
更新日期:2011-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000025881.95596.23
更新日期:2004-05-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-015-1908-z
更新日期:2015-11-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-011-0832-0
更新日期:2012-04-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-010-0247-3
更新日期:2010-07-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-016-2160-x
更新日期:2016-10-01 00:00:00