Abstract:
:The cDNA of the starch-branching enzyme I gene (IbSBEI) in the sweet potato (Ipomoea batatas) has been cloned and sequenced. The IbSBEI amino acid sequence was 81% identical to that of potato StSBEI. DNA gel-blot analyses demonstrated that at least two copies of IbSBEI are present in the sweet potato genome. IbSBEI was strongly expressed in tuberous roots. Transcript levels in the roots of single leaf cuttings were extremely low during the first 15-40 d after planting and continuously increased up to 50 d, by which time the tuberous roots had almost completely developed. This indicates that IbSBEI may work in concert with the AGPase large subunit during the primary phase of starch granule formation.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Hamada T,Kim SH,Shimada Tdoi
10.1007/s10529-006-9083-xsubject
Has Abstractpub_date
2006-08-01 00:00:00pages
1255-61issue
16eissn
0141-5492issn
1573-6776journal_volume
28pub_type
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journal_title:Biotechnology letters
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