Abstract:
:The effects of inducible materials (dexamethasone and ascorbate) on chondrogenic differentiation of rabbit chondrocytes have been examined. A hydrogel construct containing dexamethasone and ascorbate up-regulated gene expression of the cartilage matrix component of collagen to give three times the collagen content per construct at day 56 as compared to controls. Alcian Blue and Safranin-O staining revealed that these constructs also had formed more hyaline cartilage than other hydrogel constructs.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Na K,Choi SJ,Kim S,Sun BK,Woo DG,Chung HM,Park KHdoi
10.1007/s10529-007-9420-8subject
Has Abstractpub_date
2007-10-01 00:00:00pages
1453-7issue
10eissn
0141-5492issn
1573-6776journal_volume
29pub_type
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abstract::Keratinase from Pseudomonas aeruginosa KS-1 was expressed constitutively as an extracellular protein in Escherichia coli with high specific activity of 3.7 kU/mg. It was purified fourfold as a 33 kDa monomeric protein by Q-Sepharose ion exchange chromatography with a recovery of 95%. It is a serine protease with optim...
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journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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abstract::A dicistronic expression vector was constructed for Chinese hamster ovary (CHO) cells that produce both selectable marker-DHFR (dihydrofolate reductase) gene and recombinant antibody cDNA from a single primary transcript via differential splicing. The vector was derived from a pDHL vector and contained the human const...
journal_title:Biotechnology letters
pub_type: 杂志文章
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2007-11-01 00:00:00
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journal_title:Biotechnology letters
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journal_title:Biotechnology letters
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更新日期:2004-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
abstract::Polyhydroxyalkanoate (PHA) beads, recombinantly produced in Escherichia coli, were functionalized to display lipase B from Candida antarctica as translational protein fusion. The respective beads were characterized in respect to protein content, functionality, long term storage capacity and re-usability. The direct fu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-014-1735-7
更新日期:2015-04-01 00:00:00
abstract::The stability (half-life, t(½)) of the large catalase (CAT) isolated from Aspergillus terreus was decreased under acidic conditions (maximum t(½) approximately 8.5 months at pH ≤ 6) versus alkaline conditions (t(½) approximately 15 months at pH 8-12). Acidic conditions induce the dissociation of haem from CAT, as reve...
journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
abstract::Glucosylceramide and galactosylceramide were detected in three Aspergillus species: Aspergillus oryzae, Aspergillus sojae and Aspergillus. awamori, using borate-coated TLC. The cerebrosides from A. oryzae were further purified by ion exchange and iatrobeads column chromatographies with or without borate, and determine...
journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2014-12-01 00:00:00
abstract::Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9898-8
更新日期:2009-04-01 00:00:00
abstract:OBJECTIVE:To produce (S)-3-hydroxy-1-(3-(trifluoromethyl)-5,6-dihydro[1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl]-4-(2,4,5-trifluorophenyl)butan-1-one (S)-1 from 4-oxo-4-[3-(trifluoromethyl)-5,6-dihydro [1,2,4]triazolo[4,3-a]pyrazin-7(8H)-yl)-1-(2,4,5-trifluorophenyl)butan-2-one (2) by microbial bioreduction. RESULTS:A new...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2051-1
更新日期:2016-05-01 00:00:00
abstract::The combined analysis of peptide mass fingerprinting and 2-DE/MS using the induced and selected protein spots following growth of Pseudomonas sp. DU102 on benzoate or p-hydroxybenzoate revealed not only alpha- and beta-subunits of protocatechuate 3,4-dioxygenase but also catechol 1,2-dioxygenase responsible for ortho-...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9443-1
更新日期:2007-10-01 00:00:00
abstract::Metabolic regulation in Escherichia coli was studied in terms of the changes in the expression of the global regulatory genes rpoD, rpoS, soxRS, cra, fadR, iclR and arcA at three different growth phases, in batch culture. The expression of rpoS and several rpoS-dependent metabolic pathway genes, such as tktB, talA, fu...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-007-9621-1
更新日期:2008-05-01 00:00:00
abstract::An efficient synthesis of polymerizable 3'- and 5'-O-acyl-nucleoside derivatives has been developed from inosine and 2'-deoxyuridine by enzyme-catalyzed regioselective acylation with divinyl dicarboxylates. In acetone, Lipozyme (immobilized lipase from Mucor miehei) gave 5'-O-acyl-nucleoside products, and PPL (lipase ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/b:bile.0000030050.20353.a6
更新日期:2004-06-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-012-1058-5
更新日期:2013-02-01 00:00:00
abstract::Previously, we constructed a genetically modified luciferase of Photinus pyralis that generates more than 10-fold higher luminescence intensity than the wild-type enzyme. In this study, we demonstrate that this modified luciferase enables us to detect ATP at 10(-18) mol, which is almost equal to the quantity contained...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-008-9643-3
更新日期:2008-06-01 00:00:00
abstract::Hydrophobin-fused domain III of dengue envelope proteins serotypes 1 and 2 were expressed in Rachiplusia nu larvae and purified by aqueous two-phase system. This biotechnological approach of hydrophobin-fused proteins, which allowed obtaining 97.7 µg/larva of fusion protein DomIII serotype 1 and 61.4 µg/larva of fusio...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02767-2
更新日期:2020-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2583-7
更新日期:2018-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2348-8
更新日期:2017-08-01 00:00:00
abstract::The ion-exchange chromatography behavior of recombinant glucose dehydrogenase harboring pyrroloquinoline quinone (PQQGDH) was modified to greatly simplify its purification. The surface charge of PQQGDH was engineered by either fusing a three-arginine tail to the C-terminus of PQQGDH (PQQGDH+Arg3) or by substituting th...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1023/a:1026038201193
更新日期:2003-10-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-020-03039-0
更新日期:2021-02-01 00:00:00
abstract:OBJECTIVE:To evaluate the role and the molecular mechanism of miR-30d in non-small cell lung cancer (NSCLC). RESULTS:qRT-PCR was used to detect miR-30d expression in NSCLC tissues and cell lines. miR-30d was frequently down-regulated in NSCLC and its expression was associated with clinicopathological features of NSCLC...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-017-2428-9
更新日期:2017-12-01 00:00:00
abstract::Characterization of cry gene contents can help to predict the insecticidal activities of Bacillus thuringiensis isolates and in the searching of new cry genes. PCR-Single-strand conformation polymorphism (SSCP) profiling and sequencing of the highly variable cry gene regions were used to characterize cry gene content ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-006-9224-2
更新日期:2007-02-01 00:00:00
abstract:OBJECTIVES:To design and construct a novel bio-anode electrode based on the oxidation of glutamic acid to produce 2-oxoglutarate, generating two electrons from NADH. RESULTS:Efficient enzyme reaction and electron transfer were observed owing to immobilization of the two enzymes using a mixed self-assembled monolayer. ...
journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-016-2237-6
更新日期:2017-02-01 00:00:00
abstract::Biotechnology using molecular biology, biochemistry, biophysics, and computational approaches provides an alternative approach for classical pharmacological screening to look at ligand-receptor interactions and receptor specificity, which should support the design of selective drugs based on detailed structural princi...
journal_title:Biotechnology letters
pub_type: 杂志文章,评审
doi:10.1007/s10529-006-9005-y
更新日期:2006-06-01 00:00:00