Abstract:
:The promoter region of the pur operon, which contains 12 genes for inosine monophosphate biosynthesis from phosphoribosylpyrophosphate, and the purA gene, encoding the adenylosuccinate synthetase, were compared among wild-type and three purine-producing Bacillus subtilis strains. A single nucleotide deletion at position 55 (relative to translation start site) in purA gene was found in a high inosine-producing strain and in a high guanosine-producing strain, which correlates with the absence of adenylosuccinate synthetase activity in these strains. Within the pur operon promoter of high guanosine-producing strain, in addition to a single nucleotide deletion in PurBox1 and a single nucleotide substitution in PurBox2, there were 4 substitutions in the flanking region of the PurBoxes and 32 nucleotide mutations in the 5' untranslated region. These mutations may explain the purine accumulation in purine-producing strains and be helpful to the rational design of high-yield recombinant strains.
journal_name
Biotechnol Lettjournal_title
Biotechnology lettersauthors
Qian J,Cai X,Chu J,Zhuang Y,Zhang Sdoi
10.1007/s10529-006-9020-zsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
937-41issue
12eissn
0141-5492issn
1573-6776journal_volume
28pub_type
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journal_title:Biotechnology letters
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更新日期:2016-01-01 00:00:00
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更新日期:2007-11-01 00:00:00
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更新日期:2016-05-01 00:00:00
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更新日期:2011-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-09-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-018-2507-6
更新日期:2018-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2015-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1007/s10529-014-1616-0
更新日期:2014-12-01 00:00:00
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更新日期:2017-01-01 00:00:00
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doi:10.1007/s10529-010-0227-7
更新日期:2010-06-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2009-10-01 00:00:00
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更新日期:2015-03-01 00:00:00
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更新日期:2003-02-01 00:00:00
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更新日期:2018-01-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2003-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-04-01 00:00:00
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更新日期:2004-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2011-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-08-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
doi:10.1007/s10529-019-02767-2
更新日期:2020-03-01 00:00:00
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journal_title:Biotechnology letters
pub_type: 杂志文章
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更新日期:2003-11-01 00:00:00